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Birsa Agricultural University, Ranchi

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Subject: Veterinary Public Health × End date: 1997 × Start date: 1993 × Type: Thesis ×
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    ThesisItemOpen Access
    Studies on bacillus Enterotoxins and their Pathogenesis
    (Birsa Agricultural University, Ranchi, 1997) Malik Mehboob Willayat; K.G.Narayan
    1. Six varieties of rice collected from north, south, east and west of Ranchi city were found contaminated to an extent of 50.00, 56.25, 81.25, 87.50, 100.00 and 100.00 percent, respectively, for Sonachur, Rice King, Mansuri, Parmal, Gandevta and Bundu. 2. Percentage of sample bearing heat resistant strains (100°C, 20 minutes) was 7.29 indicating that cooked rice may not be sterile always as is presumed. 3. The biotyping of the isolates revealed that the rice was contaminated with biotypes 7(28.94%), 6( 23,68 %), 5(18,42%) and 2(13.15%). This would help to understand the epidemiology of food poisoning due to Bacillus cereus. 4. Most of the isolates from rice (65.78%) did not ferment xylose and the findings are in accordance with the European reports. 5. The field isolates varied in production of the type of enterotoxins. 45.83 percent isolates produced both emetic as well as diarrhoeal type enterotoxins compared to 33.33 and 20.80 percent producing only emetic and diarrhoeal enterotoxins, respectively. 6. Shake-Cultures incubated at 30-32°C for 5-12 hours was ideal for optimal production of diarrhoeal enterotoxin as tested by VPR and MLT. 7. Bacillus cereus metabolites viz. phospholipase C, hemolysins and the lethal toxins when separated by gel filtration and other Methods did produces VPR or MLT in Vivo but Produced strong in Vitro lecithinovitelline reaction and hemolysis.
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    ThesisItemOpen Access
    Studies on Bacillus Cereus Isolated from Milk and Milk Products
    (Birsa Agricultural University, Ranchi, 1997) Miss Elanbam Motina; Md,Kalimuddin
    Altogether 250 samples of milk and milk products from urban and suburban areas of Ranchi were tested, out of which 81 samples revealed contamination with Bacillus cereus and the percent positivity was 32.40. Higher percent of contamination was found in urban (36.00) than in suburban (28.80) areas. However, statistical analysis revealed that the difference was not significant. Results of studies on milk and milk products also showed similar pattern of contamination with Bacillus cereus. Of the milk products tested, highest percentage (41.37%) of rasogula samples followed by milk powder (40.00%) pera and berfi each (26.31%) were found contaminated. The more samples of raw milk from urban (35.71%) than from suburban (25.00%) areas were found positive for Bacillus cereus. The average presumptive counts of Bacillus cereus in the samples of milk and milk products drawn from urban areas were more than in the samples of suburban areas. but this difference was statistically not significant. Majority of the positive samples of milk and milk products had a presumptive and confirmatory counts of <4.000 cfu/ml or g in suburban and urban areas. Confirmatory counts of Bacillus cereus was 5.000 cfu/g and above in milk products from urban area. Only two samples from suburban area were found contaminated at the level of 8.000 to 9.000 cfu/g of berfi.