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Birsa Agricultural University, Ranchi

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Subject: Veterinary Physiology × End date: 1997 × Start date: 1993 × Type: Thesis ×
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    ThesisItemOpen Access
    In Vitro Maturation and In Vitro Fertilization of Caprine oocytes and its transfer in Black Bengal Goats
    (Birsa Agricultural University, Ranchi, 1997) Debashis Chakraborty; J.N.Pandey
    A large number of embryos can be produced by IVM-IVF technique using abattoir ovaries for research and commercial purpose at a very low cost. The technique involves collection, maturation, fertilization and early embryonic development in suitable media. Oocytes were retrieved from the follicles of goat ovaries collected from slaughter house. The maximum retrieval of cocytes (72.70%) was obtained by dissection technique followed by 61.89% through aspiration and 53.31% through puncture technique. Highest retrieval (38.53%) of good quality pocytes was found through puncture technique followed by dissection (32.42%) and aspiration (25.74%) technique. The fair quality of oocytes were retrieved maximum through aspiration technique (52.23%) followed by dissection (46.93%) and puncture (41.40%) technique. The number of retrieved oocytes/ovary were 5.41 +0.33, 4.54 + 0.14 and 4.09 + 0.80 by dissection, aspiration and puncture technique respectively. Oocytes were cultured in TCM-199 + 10% EDS (medium 1) and TCM - 199 + 10% EDS + 10 IU/ml each of PMSG and HCG (medium 2). Maturation upto metaphase - II indicated a significantly higher proportion of oocytes 65.56% reaching metaphase II in medium 2 compared to 53.79% at the same stage in medium 1. Oocytes from larger follicles have better chance of embryonic development than their smaller counterpart. The incubation time
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    ThesisItemOpen Access
    Superovulation and Embrya Collection from Prepubertal Goats and its transfer in Adult Block Bengal Goats
    (Birsa Agricultural University, Ranchi, 1997) Nighat wafai; S.K. Sinha
    Thirty Black Bongal Goats (12 propubortal about 710 months old and 18 adult 112 to 3 years old) were taken for the present study. Out of 12 prepubertal goats, six were subjected to a super ovulatory treatment comprising of PMSG and HCG (Gr.l) and remaining six received no treatment and served as controls (Gr.1l) Eighteen adult goats were also divided into Group-III, Group-IV and Group-V with each having six animals. The goats under Group III also received a super ovulatory treatment (PMSG and HCG) for comparative studies whereas animals under Group IV and Group V served as recipients for Group I and Group III synchronization. Studies receiving PGF, alpha alone for estrus on synchronization, superovulation, embryo collection and its evaluation were carried out in prepubertal and adult goats. Normal transferable embryos were transferred in the adult recipient goats with a synchronous stage of estrus comparable to donor goats. The percentage of success of embryo transfer was evaluated on the basis of kids born. The observations thus recorded are summarized below. The average time interval between PMSG administration and onset of estrus in prepubertal and adult goats was 24.70+3.60 and 27.00+1.29 hours respectively. The variation in the time interval was, however, non-significant statistically. The control group didn't show signs of estrus during the comparative studies.