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Birsa Agricultural University, Ranchi

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Subject: Veterinary Microbiology × End date: 1997 × Start date: 1993 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Studies on Local Isolate of Salmonella Gallinarum With Special Reference to the Pathogenicity and Differential Diagnosis
    (Birsa Agricultural University, Ranchi, 1993) Arun Prasad; J.P. Soman
    Fowl typhoid is an important contagious disease of poultry inflicting heavy economic loss by way of heavy morbidity and mortality. In recent past its occurrence has also been reported from Chotanagpur region of Bihar. The present study was undertaken to identify the causative agent with respect to its biochemical characteristics and antigenic nature and to assess the role of immune system in protection against experimental infection. The causative agent of the disease S. gallinarum was successfully isolated from dead birds received for postmortem examination in the Department of Pathology from different poultry farms located nearby the Institute. In addition, the isolation was also made from the birds sent from the poultry farms of Jamshedpur and Ramgarh. The organism was biochemically confirmed as S. gallinarum and its serological confirmation was made by National Salmonella Centre, Hygiene Institute, Hamburg, West Germany. It had 9,12 somatic antigens without flagellar antigenicity. When subjected to biochemical test the isolate was found similar to reference strain of S. gallinarum except that it had delayed positivity to HS gas production and citrate utilization. The whole cell lysate of S. gallinarum when subjected to SDS-PAGE technique revealed a total of 11 protein bands of which six bands were common to S. pullorun. The local isolate was found identical to reference S. gallinarum strain. Since both serotypes were found to have dis-similarity in cellular pins, SDS-PAGE technique can be employed for differential diagnosis. This technique has been used for the first time in the present study.