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Birsa Agricultural University, Ranchi

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19862
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Subject: Veterinary Microbiology × Author: Sinha, OM PRKASH × End date: 2019 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDIES ON CONTAGIOUS ECTHYMA VIRUS WITH SPECIAL REFERENCE TO ITS CYTOPATHIC , PHYSICO- CHEMICAL AND IMMUNOGENIC CHARACTERISTICS
    (Birsa Agricultural University, 1986) Sinha, OM PRKASH; SOMAN, J.P.
    In the present study, using a local isolate of CEV described as "Ranchi" strain, attempts were made to find out the host range and to grow the virus in different cell cultures to study its cytopathogenicity and physico-chemical characteristics. Among other things, an immunological comparison was also made with SPV and GPV to select out a test for the differential diagnosis of pox infections of sheep and goats. The CEV was successfully cultivated in GK, SK and CHF cell cultures. The growth of the virus in cell cultures vas confirmed by inoculating the virus back to susceptible goats. The virus was found adapted by serial cultivation in GK cell cultures. The titre of the virus in this cell culture at 5th passage level was 5X106.5 TOID/ml, whereas it rose to 5x107.5 TOID50/m1 at 10th passage level. The granulation was observed to be the main characteristic sign of the OPE in GK and SK cell cultures, whereas rounding of cells was observed to be the most striking feature of the CPE in CEF cell culture. In stained preparations, eosinophilia (M&E) or metachromatic (KGG) inclusions with clear halos were observed in infected cells at 24 hours PI and onwards. The nuclear vacuolation as reported with GPV and SPV was not observed at any stage of infection. Similarly nuclear inclusions as reported with GPV were not noted with CEV. In some cells several organized small inclusions interspersed in the cytoplasm, in addition to the large mass, seemed to be another characteristic feature of the CEV. Tracts of infected
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON CONTAGIOUS ECTHYMA VIRUS WITH SPECIAL REFERENCE TO ITS CYTOPATHIC , PHYSICO- CHEMICAL AND IMMUNOGENIC CHARACTERISTICS
    (Birsa Agricultural University, 1986) Sinha, OM PRKASH; SOMAN, J.P.
    In the present study, using a local isolate of CEV described as "Ranchi" strain, attempts were made to find out the host range and to grow the virus in different cell cultures to study its cytopathogenicity and physico-chemical characteristics. Among other things, an immunological comparison was also made with SPV and GPV to select out a test for the differential diagnosis of pox infections of sheep and goats. The CEV was successfully cultivated in GK, SK and CEP cell cultures. The growth of the virus in cell cultures vas confirmed by inoculating the virus back to susceptible goats. The virus was found adapted by serial cultivation in GK cell cultures. The titre of the virus in this cell culture at 5th passage level was 5x106.5 TOID/ml, whereas it rose to 5x107.5 TOID50/ml at 10th passage level. The granulation was observed to be the main characteristic sign of the CPE in GK and SK cell cultures, whereas rounding of cells was observed to be the most striking feature of the CPE in CEF cell culture. In stained preparations, eosinophilic (B) or metachromatic (MGG) inclusions with clear halos were observed in infected cells at 24 hours PI and onwards. The nuclear vacuolation as reported with GPV and SPV was not observed at any stage of infection. Similarly nuclear inclusions as reported with GPV were not noted with CEV. In some cells several organized small inclusions interspersed in the cytoplasm, in addition to the large mass, seemed to be another characteristic feature of the CEV. Tracts of infected cell in sheet as recorded after staining, indicate cell-to cell spread of the virus through cytoplasmic bridges.