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Birsa Agricultural University, Ranchi

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Subject: Veterinary Epidemiology and Preventive Medicine × End date: 1997 × Start date: 1993 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Biotyping and Serotyping of Bacillus Cereus Isolated from fried Rice and Chawmin
    (Birsa Agricultural University, Ranchi, 1993) Rajendra Yadav; K.G.Narayan
    Bacillus cercus has recently emerged as one of the most important food borne pathogen in India. It is most commonly associated with cereal foods causing diarrhea and vomition in human being. As compared to other food borne pathogens relatively little work has been done on . cercus particularly with fast foods. Therefore, the present investigation was undertaken to see the incidence of this organism in chawmin and fried rice. Skimmed milk powder was also included in this study. Altogether 226 samples consisting of chowmin (100), fried rice (100) and skimmed milk powder (26) were examined. The result has been presented in log10 scale. Altogether 23.0, 24.0 and 69.23 per cent of the samples of chaumin, fried rice and skimmed milk powder were positive for cereus and the counts ranged between zero to 3.9193/0, zero to 3.6182/g and zero to 3.6812/g with an average of 0.6453 0.1225, 0.7394 0.1400 and 1.9386 0.2736/s, respectively. Mannitol egg yolk polymixin agar appears to be a satisfactory selective and differential medium for cereus which is inhibitory to many bacteria and only a limited group of organisms grew on it. Viz. S. aureus, Serratia marcescens and P. vulgaris. The number of such bacteria was also estimated and designated as bacteria other than cercus. Altogether 74.0, 64.0 and zero per cent of the samples of choumin, fried rice and skimmed milk powder were positive for bacteria other than cercus and the count ranged from zero to 5.4771, zero