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Birsa Agricultural University, Ranchi

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Subject: Forest Product and Utilisation × End date: 2023 × Start date: 2023 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    GEO-REFERENCING AND ASSESSMENT OF MORPHO-BIOCHEMICAL DIVERSITY OF KALMEGH UNDER IN VIVO CONDITIONS IN JHARKHAND
    (Birsa Agricultural University, Ranchi, 2023) MADHURA M S; Jai Kumar
    In light of the global utilization of wild medicinal plants for pharmaceutical purposes, it has become imperative to search for medicinal plants that yield high biochemical contents. The secondary metabolites produced in Kalmegh are the result of environmental and edaphic conditions in which it grows and it becomes essential to identify the reasons that contribute towards maximum biochemical production in Kalmegh. The agro climatic sub-zone V of Jharkhand is considered as relatively superior for higher yield and biochemical contents production of Kalmegh, hence the present research was undertaken to corroborate the hypothesis and assist the region in securing a Geographical Indication (GI) tag with the following objectives (i) To assess morpho-biochemical diversity of Kalmegh in all three agroclimatic zones of Jharkhand under in-vivo conditions. (ii) To conduct geo-referencing of morpho-biochemical traits of Kalmegh in all three agroclimatic zones of Jharkhand under in-vivo conditions. (iii) To analyze the traits association studies with local climatic and edaphic conditions to screen out striking factors contributing towards the uniqueness of Kalmegh. The experiment comprised a total of 50 treatments which consisted of three replications and 30 plant samples in each treatment. The number of treatments from agro climatic sub-zone V, IV and VI were forty, five and five respectively. For each location, the coordinates and altitude were recorded, plant growth and yield parameters were measured and soil samples were collected for further analysis. HPLC analysis of plant samples was carried out at CIMAP, Lucknow. Meteorological data was collected for the plant growth period until harvest. Geo-referencing and grid mapping of diversity was carried out using DIVA-GIS (Version 7.5). Statistical analysis was carried out using OPSTAT and INDOSTAT. Highly significant difference was observed in mean leaf length, mean leaf width, mean length of internodes, mean number of internodes, andrographolide percentage and neo-andrographolide percentage in Kalmegh germplasm collected from agro climatic sub-zone IV & VI while, significant difference was observed in mean plant height, mean stem diameter, mean number of primary branches, mean fresh weight/plant, mean dry weight/plant, mean leaf weight/plant, mean stem weight/plant, and mean leaf stem ratio between the germplasm collected from agro climatic sub-zone IV and VI. Highly significant difference was observed in mean number of primary branches, mean leaf length, mean leaf width and mean length of internodes in Kalmegh germplasm collected from agro climatic sub-zone V & VI while, significant difference was observed in mean number of internodes and neo-andrographolide content of Kalmegh between the germplasm collected from agro climatic sub-zone V and VI. Highly significant difference was observed in mean leaf lamina width between the germplasm collected at agro climatic sub-zone IV & V. Maximum plant height was recorded at T2-Kujram (71.14 cm) followed by T4-Kujram (66.36 cm), while maximum stem diameter was recorded at T2-Kujram (5.35 mm) followed by T1-Tarub (4.79 mm). Maximum number of primary branches/plant was recorded at T5-Dangiadagh (4.57) followed by T4-Kujram (4.40) and maximum leaf lamina length was recorded at T4-Kujram (4.72 cm) followed by T5-Dangiadagh (4.56 cm). Maximum leaf lamina width was recorded at T4- Kujram (2.04 cm) followed by T10-Remta (2.00 cm) and maximum length of internodes was recorded at T2-Kujram (4.43 cm) followed by T4-Kujram (4.35 cm). Maximum number of internodes was recorded at T5-Dangiadagh (17.40) followed by T4-Kujram (16.73) while maximum fresh weight/plant was recorded at T2-Kujram (61.17 g) followed by T4-Kujram (59.90 g). Maximum dry weight/plant was recorded at T2-Kujram (13.68 g) followed by T1-Tarub (13.29 g) and maximum leaf weight/plant was recorded at T1-Tarub (6.85 g) followed by T2-Kujram (6.35 g). Maximum leaf stem ratio was recorded at T36-Dulli (1.46) followed by T1-Tarub (1.43). Maximum andrographolide content was recorded at T1-Tarub (1.98%) followed by T3-Khunti-Taimara road (1.87%) and maximum neo-andrographolide content was recorded at T1-Tarub (0.41%) followed by T18-Chikor (0.30%). Correlation analysis of andrographolide content of Kalmegh with growth and yield parameters, climatic parameters, soil physical and chemical parameters revealed andrographolide content showed highly positive significant correlation with leaf stem ratio (0.762), leaf weight per plant (0.640), leaf width (0.578), dry weight per plant (0.567), stem weight per plant (0.550), fresh weight per plant (0.540), plant height (0.524), leaf length (0.512), and number of internodes (0.508). Highly significant positive correlation of andrographolide content was seen with the altitude of the collection area (0.485). Significant positive correlation of andrographolide content was observed with sand percentage of soil (0.399) and bulk density of soil (0.415), but significant negative correlation with water holding capacity of soil (-0.457). Further andrographolide content showed highly significant positive correlation with organic carbon (0.559) and available phosphorus of soil (0.631) and significant positive correlation with available nitrogen (0.427) and available potassium of soil (0.420). Regression analysis of growth parameters showed that the plant height, number of internodes, dry weight/plant and leaf weight/plant of Kalmegh had significant positive impact on its andrographolide content while, regression analysis of climatic factors with growth, yield and biochemical parameters showed significant impact of leaf weight/plant, mean temperature and altitude on its andrographolide content. Regression analysis of soil physical parameters showed significant impact of leaf width and leaf weight/plant on andrographolide content while, regression analysis of soil chemical parameters showed significant impact of plant height, leaf weight/plant, leaf stem ratio, organic carbon of soil and available potassium of soil on its andrographolide content. Path value analysis of biochemical factors with growth and yield factors of Kalmegh revealed the highest positive direct impact of dry weight/plant (5.178) followed by leaf weight/plant (4.161) on its andrographolide content. Again, path value analysis of climatic factors revealed maximum positive direct impact of leaf weight/plant (1.915) followed by altitude (0.514) on its andrographolide content. Path value analysis of soil physical parameters revealed maximum positive direct impact of leaf weight/plant (2.918) followed by plant height (0.436) on andrographolide content, while path value analysis of soil chemical parameters revealed maximum positive direct impact of leaf weight/plant (2.713) followed by plant height (0.559) on its andrographolide content. As regards to the first objective namely, morpho-biochemical diversity of Kalmegh in all three agro climatic sub-zones of Jharkhand under in-vivo conditions, highly significant difference was observed between ACZ IV & VI in leaf lamina length, leaf lamina width, length of internodes, number of internodes, andrographolide content and neo-andrographolide content of Kalmegh, whereas, highly significant difference was observed between ACZ V & VI in number of internodes and neo-andrographolide content of Kalmegh. Significant difference was observed between ACZ IV & VI in plant height, stem diameter, number of primary branches/plant, fresh weight/plant, dry weight/plant, leaf weight/plant, stem weight/plant, and leaf stem ratio of Kalmegh. Significant difference was observed between ACZ V & VI in number of primary branches/plant, leaf lamina length, leaf lamina width and length of internodes of Kalmegh, whereas, significant difference between ACZ IV & V was observed only in leaf lamina width of Kalmegh. Superior germplasm of Kalmegh identified with high andrographolide content were T1-Tarub (1.98%), T20 -Bhanrra (1.96%), T3-Khunti-Taimara road (1.87%), T41-Patratu valley (1.87%). Superior germplasm of Kalmegh identified with high neo-andrographolide content were T1-Tarub (0.41%), T18- Chikor (0.30%), T19-Bhanrra (0.30%), T20-Bhanrra (0.30%). As regards to the second objective namely, geo-referencing of morpho-biochemical traits of Kalmegh in all three agro climatic sub-zones of Jharkhand under in-vivo conditions, it may be concluded through grid mapping of 50 Kalmegh germplasm that the agro climatic sub-zone V exhibited a higher level of superiority with respect to growth, yield and biochemical contents when compared to the other two sub-zones of Jharkhand. As regards to the third objective namely, traits association studies with local climatic and edaphic conditions, following conclusion can be drawn, andrographolide content of Kalmegh showed highly significant positive correlation with altitude (0.485), which shows that the biochemical content may be found in higher amount at higher altitudes. Andrographolide contents of Kalmegh showed positive significant correlation with bulk density (0.415) and sand percentage (0.399). The andrographolide content of Kalmegh showed highly significant positive correlation with organic carbon (0.559) and available phosphorus (0.631), and significant positive correlation with available nitrogen (0.427) and available potassium (0.420), whereas andrographolide content showed a negative non-significant correlation with soil pH, silt percentage and clay percentage of soil. Analysis of 50 Kalmegh germplasm revealed that the most significant factors contributing to the diversity of Kalmegh were fresh weight/plant (36.73%), dry weight/plant (11.76%), and leaf weight/plant (10.04%) respectively. The current study suggests that the agro climatic sub-zone V in Jharkhand exhibits superior growth & yield as well as biochemical production of Kalmegh. To strengthen these findings, further investigations, such as expanding the sample size in experiments and conducting molecular analyses on Kalmegh can be conducted. These analyses will contribute to the assertion that sub-zone V is the most suitable candidate for obtaining a Geographical Indication (GI) tag in relation to biochemical, growth and yield production of Kalmegh.