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Birsa Agricultural University, Ranchi

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20221
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Subject: Forest Product and Utilisation × Author: KUMAR, AMIT ×
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    ThesisItemOpen Access
    Studies on genetic divergence of Ashwagandha [Withania somnifera (L.) Dunal] genotypes through its quantitative traits
    (Birsa Agricultural University, Ranchi, 2022) KUMAR, AMIT; Kumar, Jai
    Keeping in view the importance of genetic diversity in optimum genetic resource management strategies, source of desirable allele and assist plant breeders in developing climate resilient varieties, a systematic research trial was undertaken to quantify extent of divergence of different Ashwagandha genotype through its qualitative and quantitative traits, at AICRP (M&APs) research farm, B.A.U Ranchi with the following objectives; To find out the extent and magnitude of diversity in selected Ashwagandha genotypes, To determine the percentage contribution of quantitative parameters creating maximum diversity in selected Ashwagandha genotypes, To identify potential parents of Ashwagandha for hybridization programme to develop hybrid/variety of high yield potential and To select promising genotypes of Ashwagandha suitable for agro-climatic conditions of Jharkhand. Research trail was laid out in Randomised block design with 17 treatment & 3 replications of Withania somnifera genotype collected from DMAPR, Anand, Gujarat. Different qualitative & quantitative parameters were measured as par NBPGR guideline. The replicated data of all the quantitative traits of 17 genotype of Ashwagandha was subjected to Analysis of variance, Genetic divergence analysis, Principal component analysis, Jaccard’s similarity coefficients, Shannons diversity index and Duncans multiple range test. 9 germplasm of Ashwagandha showed erect plant growth habit, while 8 had semierect. The leaf shape of 10 germplasm was ovate, while 7 germplasm had ovate-rounded. Three type of leaf colour were found namely light green (9), greenish yellow (4) and pale green (4). The root colour of 11 germplasm was cream, while 6 germplasm had whitish cream. Two type of root fracture were found namely fibrous (12) and non-fibrous (5). Internal root colour of 10 germplasm was cream and rest 7 germplasm were white. Two type of inflorescence were found namely umbellate cymes (9) and axillary fascicles (8). Three type of flower colour were found namely dull yellow (9), green (6) and yellow (2). Berries colour of 10 germplasm was orange and 7 had red colour. Significant difference was noticed in the plant height and maximum value was recorded for RAS-45 (96.58 cm) and minimum for RAS-65 (28.29cm). Significant difference was noticed in stem diameter at collar region and maximum value was noticed for JC-310620A (3.02 cm) and minimum for RAS-65 (0.77 cm). In case of number of primary branches/plant, maximum value was noticed for RAS-34 (4.52) and minimum for RAS-65 (2.75). Significant difference was noticed in leaf length and its maximum value was recorded for RAS-45 (8.65 cm) and least for AAU (4.59 cm). Significant difference was noticed in leaf width and its maximum value was recorded for RAS-45 (3.4 cm) and least by AAU (1.7 cm). Maximum berries diameter was recorded for MWS-334 (0.69 cm) which varied significantly with its minimum value of RAS-31 (0.51 cm). Maximum root length was recorded for RAS-45 (28.0 cm) and minimum for RAS-65 (10.5 cm). Significant difference was noticed in root diameter and its maximum value was recorded for RAS-45 (2.53 cm) and minimum for RAS-65 (0.94 cm). Significant difference was observed for number of secondary roots/plant and its maximum value was recorded for MWS-334 (12.30) and minimum for RAS-65 (7.27).