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Birsa Agricultural University, Ranchi

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20151
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Subject: Animal Reproduction and Gynecology × Author: Mehta, Arjun Kumar × End date: 2019 × Start date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    EFFECT OF CAFFEINE CITRATE AND PROSTAGLANDINF2α AS ADDITIVES ON RAM SEMEN PRESERVATION
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2015) Mehta, Arjun Kumar; Sinha, M. P.
    The present study was conducted to evaluate the seminal attributes of six Chottanagpuri rams and to see the effect of different dilutors on preservation at refrigerator temperature. A schedule of twice weekly collection of semen per ram was followed. Ejaculates having three plus initial motility were finally diluted with TCFY extender in 1:1 ratio containing additives viz. caffeine citrate and ProstaglandinF2α at the rate of 2mM/ml, or 4 µg/ml. separately. Extended semen samples were preserved in refrigerator at 4-50C and evaluated for the various sperm characteristics viz., progressive motility, pH, live sperm percentage and sperm abnormality at 24, 48, 72 and 96 hours of preservation. Data were statistically analyzed for assessing their preservability. The results obtained have been summarized below: 1. The colour of the Chottanagpuri ram semen was found to be creamy or creamy white. 2. The mean value for different seminal attributes viz., volume (ml.), pH, mass motility, sperm concentration (millions/ml.), live sperm percentage, head, mid piece and tail abnormality percentage in neat semen was 0.69±0.01 ml, 6.66±0.02, 3.81±0.07 (0-5 score), 4043.06±106.76 millions/ml, 81.61±0.23 percent, 0.49±0.05 percent, 0.60±0.05 percent and 5.94±0.13 percent, respectively. 3. Ejaculate volume, pH, mass motility, sperm concentration and live sperm percentage ranged between 0.67±0.03 to 0.72±0.03 ml, 6.62±0.03 to 6.72±0.05, 3.66±0.24 to 4.00±0.46, 3916.67±261.62 and 4291.67±345.31 millions/ml and 81.00±0.37 to 82.17±0.75 percent respectively. 4. The difference in ejaculate volume, pH, mass motility, abnormal sperm percentage, sperm concentration and live sperm percentage were found to be non significant among the rams. 5. Addition of 2mM caffeine and 4µg PGF2α had beneficial effect in respect to progressive motility at all hours of preservation. Amongst the two additives, addition of caffeine showed significantly higher progressive motility at all hours of preservation. 6. Live sperm percentages were also higher in Caffeine and ProstaglandinF2α added diluters than control at all hours of preservation. However the difference was not significant at 72 and 96 hours of preservation. 7. Percentage of head and mid piece abnormality did not differ significantly among the control, Caffeine and ProstaglandinF2α added diluters at any hours of preservation. However the effect of additives on tail abnormality were significant at all hours of preservation which was significantly lowest in caffeine added diluters followed by PGF2α added and control dilutor.