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Birsa Agricultural University, Ranchi

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20071
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Subject: Animal Reproduction and Gynecology × Author: Bharti, Manoj Kumar × End date: 2022 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDY ON SEMEN CHARACTERISTICS OF CHOTTANAGPURI RAM
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2007) Bharti, Manoj Kumar; Sinha, M.P.
    Semen was collected twice weekly by Artificial vagina method and evaluated for different spermiogram, concentrations of certain enzymes and cations in neat semen. The results obtained have been summarized below: 1. The colour of Chottanagapuri ram semen was found to be creamy or creamy white. 2. The overall mean ejaculate volume, mass-motility, sperm concentration, live sperm percentage and abnormal sperm percentage were found to be 0.648±0.027 ml, 4.222±0.144, 3793±0.134 millions/ml, 83.333±1.318 and 7.034±0.135, respectively. 3. The differences between the ejaculate volume, mass motility, sperm concentration, live sperm percentage and abnormal sperm percentage were found to be non significant among the rams. 4. The overall mean value of sodium and potassium in seminal plasma were found to be 104.183 ± 0.849 and 24.618±0.590 mEq/L. 5. The variation in the levels of sodium and potassium in the seminal plasma differed significantly (P<0.01) between the rams. 6. The overall mean AST and ALT activities in seminal plasma were revealed as 39.600±3.011 and 40.254±2.736 IU/L, which did not differ significantly among the rams. 7. The overall mean of alkaline phosphatase (AKP) in seminal plasma was 735.972±6.792 IU/L. The values did not differ significantly among the rams. 8. The overall mean value of lactic dehydrogenase (LDH) in seminal plasma was 577.766±38.502 IU/L. Analysis of variance revealed that the variations in lactic dehydrogenase levels in the seminal plasma of rams were non significant. 9. The ejaculate volume was positively correlated with live sperm percentage, abnormal sperm percentage, potassium and alkaline phosphatase (AKP) whereas it was negatively correlated with mass motility, sperm concentration, sodium, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactic dehydrogenase (LDH). All the correlations were non significant except with sodium concentration. 10. The mass motility was significantly and positively correlated with sperm concentration and live sperm percentage. Its correlation with other attributes were negative and non significant. 11. Sperm concentration was positively and significantly correlated with live sperm percentage. Its correlation with sodium was positive and negative with potassium. 12. Abnormal sperm percentage was positively but non significantly correlated with sodium, potassium, lactic dehydrogenase (LDH) and alkaline phosphatase (AKP) enzyme. It was positively and significantly correlated with aspartate aminotransferase (AST) and alanine aminotransferase (ALT). On the basis of the findings obtained during the present study it is concluded that all the physical seminal attributes of Chottanagpuri rams are within the normal range as reported earlier in the case of other Indian breeds of ram. The seminal enzyme levels and cation levels are also within the normal range. The average values recorded for various seminal attributes and enzyme level in Chottanagpuri rams under study may be used as physiological norms for this breed in the absence of other published reports.