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Birsa Agricultural University, Ranchi

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Subject: Animal Biochemistry × End date: 2007 ×
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Now showing 1 - 5 of 5
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    ThesisItemOpen Access
    Biochemical Characterization of Different Breeds of pig with Special Reference to Metabolic Profile
    (Birsa Agricultural University, Ranchi, 2001) Madhu Kumari; R.L. Prasad
    The specific enzyme synthesis and exchange of metabolites differentiate the differential growth and quality of different breeds of pig genetically. The study on these metabolites as well as biochemical polymorphism were undertaken with a view to compare the variation in composition of metabolites as well as breed characteristics of three breeds of pig viz., Desi, T & D (Tamworth X Desi) and Tamworth. The blood samples were collected from experimental animals into three groups: Group I (Desi), Group II ( T & D) and Group III (Tamworth) from saphenous vein. Serum was harvested and stored at 0-4°C until analysed. Among the biochemical constituents total serum proteins were found to have significant (P < 0.01) higher value in Desi followed by Tamworth and T & D. This variation in Desi breed might be due to less water retention in muscle as the amount of water in lean meat is greater than fat meat. The reduced water retention properties were found to lower the yield of meat as in case of Desi as compared to Tamworth and cross bred. Beside this the present finding is suggestive of protein biosynthesis of animal species of individual or species under genetic control. The serum aspartate aminotransferase level did not show significant variations among the three breeds. However, perusal of pattern of serum aspartate aminotransferase (SAST) activity on breed type showed higher activity in Desi followed by T & D and Tamworth, suggesting less flashy muscular structure development in Desi breed of pig which might be due to involvement of gluconeogenesis. This finding of increase aspartate aminotransferase (AST) level also suggested increased AST level in Desi which might be associated with muscular dystrophy which is under genetic control, has also higher level of this enzyme like other species.
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    ThesisItemOpen Access
    Studies on Biochemical And Enzymic Properties of Skeletal Muscle Myosin of Goats ( carpa Hircus)
    (Birsa Agricultural University, Ranchi, 1994) Surendra Kumar; Dr. R.L. prasad
    The isolation and characterization of proteins of goat skeletal muscle were undertaken with a view to their molecular profile in regulation of muscle contraction through ATPase activity and losses due to proteolysis by artificial tenderizer of meat. The goat skeletal muscle myosin was isolated in pure form using different ionic strength, dissociation buffer and ammonium sulphate precipitation. The myosin contained myosin heavy chain (HC) and myosin light chain-1 (LC), myosin light chain-2 (LC) and myosin light chain-3 (LC) with 2 their molecular weights of 192.5, 23, 17.5 and 13.5 kDa respectively. The resolution of sarcoplasmic proteins into subunit components on SDS-PAGE showed the presence of enzymes and metallo proteins. The high molecular weight of unidentified proteins was present in band no.1-4. The molecular weight of 91 kDa appeared in band no.5 which was identified as phosphorylase B. The band no.6 was identified as phosphorylase B kinase with molecular weight of 77 kDa. Band no.7 was represented by AMP deaminase, phosphoglucose isomerase and pyruvate kinase with molecular weight of 62 kDa. Phosphoglycerate kinase and enclose appeared in band no.8 with molecular weight of 43 kDa. Band no.9 consisted of creatine phosphokinase, aldolase and lactic dehydrogenase having molecular weight of 34 kDa. Band no. 10 was identified as triose phosphate isomerase and phosphoglycerate mutase with molecular weight of 26 kDa.
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    ThesisItemOpen Access
    Effect of Endosulfan on Biochemical and Immunological Status of Developing Chick Embryo
    (Birsa Agricultural University, Ranchi, 1994) Pushpanjali; A.K. Pal
    A concurrent study on the effect of endosulfan (K isomer) on biochemical and Immuno chemical status of developing chick embryo has provided the following valuable Informations. 1. The possible LD 50 of endosulfan (<<-isoner) deve loping chick embryo was found to be 5.0 µg at 12 day of incubation through CAM route. 2. Lower concentration (2.0 µg) of endosulfan interferes the normal biochemical functions of the developing chick embryo. 3. The immuno-suppressive action of endosulfan in developing chick embryo warns for failure of future vaccination programme in poultry industry. 4. Use of endosulfan as poultry feed preservative should be restricted. 5. ACH/ATPase activity and SRID-Test may be used as indicator for diagnosis of the endosulfan intoxication of the developing chick embryos in hatcheries.
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    ThesisItemOpen Access
    Biochemical Role of Plant Proteolytic Enzyme ivrin on Poultry Meat
    (Birsa Agricultural University, Ranchi, 1992) Rajni Pushpa Sinku; Dr. R.L. Prasad
    The use of plant proteolytic enzyme Ivrin on poultry meat were undertaken with view to study the alternation of physico chemical characteristics and its proteolytic role on tenderness of poultry meat. The Ivrin enzyme isolated from Cucumis pubescens fruits at 60% ethanol gave more proteolytic activity as compared to 40% ethanol. The better proteolytic activity shown by ethanol 60 % and precipitation was superior used during present investigation. Among the physicochemical properties, Ivrin was found to lower the pH of meat. The pattern of pH on muscle type showed high in thigh muscle as compared to breast muscle suggesting variation in the functions, exercise and amount of glycogen content of muscles. The water holding capacity was found to be highest in broiler, followed by desi and culled bird. The Ivrin treatment increased the water holding capacity in breast muscle as compared to high muscle. The relation between pH and water holding capacity indicated decrease in pH and increase in W.H.C. in Ivrin treated thigh or breast muscle. The muscle fibre diameter was found to be higher in case of desi than broiler or culled birds. Among the muscle types thigh muscle was found to have higher M.F.D. than that of breast muscle. The thigh muscle of the desi bird were found to have exceptionally high muscle fibre diameter. The proteolytic enzyme, Ivrin was found to have no influence on muscle fibre diameter.
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    ThesisItemOpen Access
    Biochemical Markers in Equine Infertility
    (Birsa Agricultural University, Ranchi, 1989) Lt Col Kamal Krishna Prasad; S. K. Mukherjee
    1.The present study was conducted on 70 brood mares maintained at Equine Breeding stud, Babugarh, Ghaziabad, Uttar Pradesh. Prior to selection and inclusion of mares in the research programme, detailed reproductive history was obtained from the history sheets and breading records maintained at the stud farm. 2. In order to assess the present reproductive status, all the selected mares were rectally palpated and categorized under seven groups. Group I and II comprised of normal fertile mares in follicular and luteal phases respectively. Similarly the mares with history of reproductive problems/infertility subsequent to last foaling were kept under group III (follicular phase) and group IV (Luteal phase). The pregnant mares were categorized under three groups, early pregnancy (upto day 90, group IV)., mid pregnancy (day 91 to day 180, group VI), and late pregnancy (day 181 onwards, group VII). 3. Blood samples from all the selected mares were collected by jugular venipuncture at 0800 hours taking all aseptic precautions. Serum was harvested and stored at 04°c before analysis. The sera samples earmarked for progesterone estimation were stored at -20°c alongwith 0.01% sodium azide. 4. Progesterone was estimated by amplified enzyme immunoassay: technique. Estimation of enzyme (ALP and LDH), macronutrients (Ca, P and Mg) and micronutrient (Cu) were carried out by following standard procedures.