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Acharya Narendra Deva University of Agriculture & Technology, Ayodhya
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ThesisItem Open Access Isolation, evaluation and characterization of disease suppressing Plant Growth Promoting Rhizobacteria (PGPR) of lentil rhizosphere(ANDUAT, kumarganj, Ayodhya, 2021-07-31) Ahmad, Niyaj; Kumar, Dr. AdeshA total number of 16 lentil rhizobacterial isolates were isolated at Nutrient Agar medium. Only twelve isoaltes showed antagonistic activity against Fusarium oxysporum f. sp. lentil. All the antagonistic isolates produced diffusible and volatile antifungal metabolites. The isolates 10 and 14 showed a maximum antagonism 72% and 68.6% respectively. All the antagonistic isolates showed PGPR activity such as phosphate solubilization, IAA production and ammonia production. On the basis of their antagonistic and PGPR traits out of nine only two isolates, 10 and 14 were found to be more effective which were selected for SDS-PAGE and 16S rDNA molecular analysis. Strain 10 and 14 identified as Enterobacter ludwigii and Enterobacter cloacae. The protein bands of variable size are present and both isolates have similar protein banding pattern. The PCR was run with primers namely, 16s Forward 5`GGATGAGCCHAGGCCTA3` and 16s Reverse Primer 5`CGGTGTGTACAAGGCCCGG3` PCR product was run on 1.5% agarose gel for appearance of banding patterns sequence of PCR product of 16S rDNA, the electropherogram and dendogram generated which showed genetic similarity between the isolate 10 (Enterobacter ludwigii) and 14 (Enterobacter cloacae).ThesisItem Open Access Tissue culture of Grand Naine banana (Musa paradisiacal L.) And its evaluation in the field(ANDUAT, Kumarganj, Ayodhya, 2016-07-11) Singh, Avinash kumar; Singh, K.N.Banana is the most popular fresh fruit all over the world and its name comes from the Arabic word 'banan', which means finger. The scientific name of banana is Musa acuminate and Musa balbisiana. But the old scientific names of banana are Musa sapientum and Musa paradisiacal L. Bananas are rich source of carbohydrates and potassium. The present study reports a very simple one step protocol using MS with BAP for initiation, multiplication and elongation of shoot and MS with IAA for rooting of banana. The protocol raised in the present attempt could be used for the massive in vitro production of the plantlets of the banana. In the beginning, Sucker was taken as explant and inoculated in sterilized MS media.Theblackening of rhizomes during sterilization and culture were observed. The pretreatment of explants with bavistin 1gm carbendazim systemic fungicides 1gm and almox 10 mg used for 5 suckers treatment for a period of one hour before sterilization with thorough washing in running tap water and surface sterilization. Rhizome were cut into small size and treated with different reagents.Shoot initiations were observed after 3-5 days of inoculation. Single leaf development in shoot was observed in media containing 2mg/l BAP. In media containing 3.0 and 4.0mg/l BAP sprouting were observed better. Increase in concentration of BAP to 5.0mg/l along with 0.1mg/l of kinetin and IAA were growth inhibitory to number of shoot, number of leaves and length of leaves.The root initiation was activated on basal medium without any growth regulators.Rooting was stimulated when shoots were transferred to basal medium of 0.1mg/l IAA, Kinetin and activated charcoal.Rooting and shooting elongation was initiated after 20-30 days. Hardening was successfully carried out in in-vitro raised plantlets by transferring the plants in hormone free medium for period of 20 days under controlled environment condition. The tissue culture raised plantlets were transferred to soil in pre autoclaved soil mixture.Flowering started 9 to 10 months after planting. 130 fingers\bunches are found in banana cv. Grand Naine.ThesisItem Open Access Comparative studies of susceptible and tolerance varieties of pigeon pea (Cajanus cajan) using protein profiling and RAPD markers with response to Pod borer (Helicoverpa armigera) resistance(ANDUAT, Kumarganj, Ayodhya, 2016-07-11) Maurya, Rajesh Kumar; Khan, N.A.Pigeonpea seeds are important source of proteins, energy and other nutrients in the diets of large population groups around the world, forming and excellent source of lysine, methionine and tryptophan and other water soluble vitamins (riboflavin, niacin and folacin) and of the minerals phosphorus, iron and magnesium (Ramcharan and Walker, 1985). The present study involved evaluation of ten genotypes of pigeonpea including five susceptible and five resistant varieties viz., NDA-1, NDA-2, Bahar, NDA-14-6, NDA-13-6, NDA-3, UPAS-120, MAL-6, MAL-13, Cajanus scarabaeoides (wild). The work was done with 10 %, 12 %, 15 % protein profiling, 12 % native gel analysis, DNA isolation and RAPD analysis. The result obtained on above characters was analyzed for protein profiling and RAPD analysis. Gel electrophoresis of protein of pigeonpea varieties show novel band obtain at the upper portion of the 10 % acrylamide gel , these band only seen 0.455 (RM value) is pod borer tolerant and regarded as pod borer resistant responsible protein. RAPD primer was initially tested for the PCR amplification of the genomic DNA for 10varieties, but they did not amplify genomic DNA. Genomic DNA was amplified with RAPD specific primer i.e OPA-15, OPU-11 and OPA-16, but no result was found. DNA and RAPD analysis was the best tools for identification of resistant and susceptible gene for podborer (Helicoverpa armigera).ThesisItem Open Access Screening Rice Varieties For Reproductive Stage Salinity Tolerance(ANDUAT,KUMARGANJ,AYODHYA, 2015-07-06) Tiwari, Devarshi; Dwivedi, D.K.Rice is a sensitive crop to salinity stress. Salinity has been the single largest factor limiting the rice yield. Developing rice cultivars with salinity tolerance is the most efficient way to solve this problem, because salinity tolerance in rice varies with the growth stage, with vegetative and reproductive stages being most sensitive. Due to its genetic complexity and physiology of salinity tolerance in rice it is probably the most difficult trait to improve through conventional breeding. Application of molecular techniques along with conventional approach is the only option for improvement of salinity stress. The present study was under taken with the objectives (i) to screen rice varieties in field conditions for salinity tolerance, (ii) to evaluate rice varieties in controlled condition (iii) to analyze the rice varieties with molecular markers and (iv) to identify novel protein band with SDS-PAGE in rice varieties for salinity tolerance. The experiment was conducted in RBD with three replications in two sets one for salinity stress and other under control condition. Observation were recorded on days to 50% flowering, plant height, panicles bearing tillers per plant, flag leaf area(cm), number of spikelets per panicle, number of grains per panicle, spikelet fertility (%), test weight (%) biological yield per plant (g), harvest index (%), Na content, K content, Na/K ratio and grain yield (g). Varietal screening under salinity and conditions at vegetative stage revealed that NDRK 5012 was recorded as tolerant, other eight varieties were moderately tolerant.ThesisItem Open Access DEVELOPMENT OF MAPPING POPULATION FOR HEAT TOLERANCE IN WHEAT (Triticum aestivum L.) BY PHYSIO-MLECULAR APPROACHES(ANDUAT,KUMARGANJ,AYODHYA, 2017-06-30) DWIVEDI, RAKESH; PRASAD, SHAMBHOOHeat stress due to the global warming is one of major constraints in the way of higher wheat productivity. With an objective “Development of mapping population for heat tolerance in wheat (Triticum aestivum L.) by physio- molecular approach.” an experiment was conducted with seventeen wheat genotypes namely Halna, PBW-343, HD-2733, K- 8962, NW-1067, RAJ-3765, K-9006, RAJ-4134, DBW-22, PBW-373, NW-1012, NW-2045, HD-2808, LOK-54, RAJ-4101, HI-1544 and NW- 4004 was conducted at instructional farm and in the laboratory of P.M.B. and G.E., N.D.U.A.&T, Kumarganj, Faizabad during rabi season of 2016- 2017. Heat stress was induced by delayed sowing of 34 days from normal date of sowing (29 December) so that grain filling stage of wheat could experience severe heat stress.ThesisItem Open Access TISSUE CULTURE OF RICE VARIETIES IR 64 AND SWARNA(ANDUAT,KUMARGANJ,AYODHYA, 2007-01-06) PRIYA, SHIVAM; SINGH, K.N.To develop an efficient tissue culture protocol for callus induction and regeneration in two popular indica cultivars i.e. IR 64 and Swarna was undertaken. The explants used for initiation of culture were mature seeds. For callus induction in Swarna and IR 64, MS (Murashige and Skoog, 1962) media was used with different concentration of 2, 4-D, Kinetin, L-proline and Casine hydrolysate in 10 combinations. Best callus induction in terms of friability and growth in Swarna were obtained in the medium having combinations of MS media supplemented with 2, 4-D (2 mg/ l) + Kinetin (0.4 mg/ l) + L-proline (500 mg/ l) + Casein hydrolysate (300 mg/ l) + maltose (30 g/ l) + agar-agar (0.8 % w/v) with 87 % callus induction percentage, whereas in IR-64, it was found best with MS media supplemented with 2, 4-D (2 mg/ l) + L-proline (500 mg/ l) + Casein hydrolysate (300 mg/l) + maltose (30 g/ l) + agar-agar (0.8 % w/v) with 78 % callus induction percentage.ThesisItem Open Access Isolation and characterization of Rhizoctonia solani causal agent of sheath blight of paddy(ANDUAT, Kumarganj, Ayodhya, 2020) Minakshi; Adesh KumarAddThesisItem Open Access Analysing seed protein and DNA of hulled vs. hull-less barley varieties using 2-D and RAPD techniques(ANDUAT, Kumarganj, Ayodhya, 2015) Navneet Kumar; Singh, K.N.AddThesisItem Open Access Screening of rice (Oryza sativa L.) mapping population for submergence and drought tolerant traits(ANDUAT, Kumarganj, Ayodhya, 2020) Mishra, Vishwash Kumar; Prasad, Shambhoo