Isolation, evaluation and characterization of disease suppressing Plant Growth Promoting Rhizobacteria (PGPR) of lentil rhizosphere

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ANDUAT, kumarganj, Ayodhya
A total number of 16 lentil rhizobacterial isolates were isolated at Nutrient Agar medium. Only twelve isoaltes showed antagonistic activity against Fusarium oxysporum f. sp. lentil. All the antagonistic isolates produced diffusible and volatile antifungal metabolites. The isolates 10 and 14 showed a maximum antagonism 72% and 68.6% respectively. All the antagonistic isolates showed PGPR activity such as phosphate solubilization, IAA production and ammonia production. On the basis of their antagonistic and PGPR traits out of nine only two isolates, 10 and 14 were found to be more effective which were selected for SDS-PAGE and 16S rDNA molecular analysis. Strain 10 and 14 identified as Enterobacter ludwigii and Enterobacter cloacae. The protein bands of variable size are present and both isolates have similar protein banding pattern. The PCR was run with primers namely, 16s Forward 5`GGATGAGCCHAGGCCTA3` and 16s Reverse Primer 5`CGGTGTGTACAAGGCCCGG3` PCR product was run on 1.5% agarose gel for appearance of banding patterns sequence of PCR product of 16S rDNA, the electropherogram and dendogram generated which showed genetic similarity between the isolate 10 (Enterobacter ludwigii) and 14 (Enterobacter cloacae).