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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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2004 - 200912010 - 20122
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Author: Sanjeev Kumar × Start date: 1994 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Purification of cellulase and xylanase enzymes produced from potential microorganisms isolated from compost for industrial application
    (YSPU, 2012) Sanjeev Kumar; Sharma, Nivedita
    The present investigation was carried out to isolate, screen and identify the most efficient cellulolytic and xylanolytic microorganisms from compost. Enzyme production, optimization, purification, characterization, kinetics, cellulase/xylanase gene encoding and scale up studies were performed with selected strains to recommend their use for industries. In total 102 microorganisms including 93 bacteria and 9 fungi were isolated. Among them, three bacteria SH8, SH0 and BM-1 and two fungal isolates i.e. T2 and W2were screened for cellulase and xylanase enzyme production studies. The bacterial isolates were identified as B. amyloliquefaciens, B. tequilensis and B. subtilisrespectively by 16S rRNA PCR technique and registered in NCBI under accession no. JX129360.1, JX129359.1 and JX129361.1 while fungiwere identified as T. longibracheatum and R. oryzae by ITS 5.8S rRNA technique and being assigned accession no. JX213811.1 and JX213812.1. in NCBI. Cellulase and xylanase enzymes were optimized through classical approach one factor at a time (OFAT) as well as response surface methodology (RSM) under submerged fermentation varying medium, pH, temperature, inoculum size, carbon source, substrate concentration and incubation time and in solid state fermentation using different pretreatments i.e. pH, temperature, moisture ratioand incubation time. The percent increase in enzyme activity obtained after optimization of different process parameters was 204.51% for cellulase of B. subtilis BM-1, 222.91% and 210.52% for xylanase of B. amyloliquefaciensSH8 and B. tequilensisSH0, respectively in SmF. The increase in cellulase and xylanase of T. longibracheatumT2 was recorded 63.65% and 95.96% while in R. oryzaeW2 it was 107.38% and 125.64%, respectively under SSF. The purification of hydrolytic enzymes was proceeded following multistep purification technique using ion exchange chromatography and gel exclusion chromatography and the molecular weight of partially purified xylanase enzymes of B. amyloliquefaciensSH8, B. tequilensisSH0 and T. longibracheatumT2 were found in the range of 14 kDa to 93.4 kDa, respectively. The partially purified xylanase of both bacterial and fungal origin were further characterized by studying, the effect of various parameters viz. the effect of pH, temperature, metal ions, substrate specificity, substrate concentrations and their kinetic parameters were derived. The partially purified thermostable xylanaseof bacterial origin was active at 90 o C, pH 6.0, showed high activity on xylan containingsubstrates and depicted cellulase free nature. The K m and V max of B. amyloliquefaciens SH8 xylanase for birch wood xylan were 166.67 µmol/mg/min and 5.83 mg/ml, while for B. tequilensisSH0 were 166.66 µmol/mg/min and 10.99 mg/ml. Similarly, partially purified enzyme of T. longibracheatumT2 was optimally active at 60 o C, pH 5.0 and showed high activity on xylan containing substrates. The K m and V max of T. longibracheatum T2 xylanase for birch wood xylan were 125.0 µmol/mg/min and 1.55 mg/ml. Cellulase and xylanase abilities of the isolates were targeted by amplification of eglS and Xyn genes. Optimum conditions i.e. inoculum age, inoculum size, aeration rate and agitation rate were explored at pilot scale for xylanase enzyme by B. amyloliquefaciens SH8. The inoculum age of 4h, inoculum size @ 10% concentration, 1.0vvm and agitation rate of 200 rpm were found best for production of xylanase enzyme in 7.5 L bioreactor. Mathematical model based on experimental results for xylanase production was proposed. The production of xylanasewas found growth associated. The model consists ofa set of ordinary differential equations taking into account the bacterial growth, substrate utilization and xylanase production with time.
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    ThesisItemOpen Access
    Studies On Distribution, Growth And Biological Yield Potential Of Bamboos In Himachal Pardesh
    (Dr Yashwant Singh Parmar University of Horticulture and Forestry;Solan, 2010) Sanjeev Kumar; Bhardwaj, D R
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    ThesisItemOpen Access
    Effect Of Drip Irrigation, Fertigation And Rootstocks On The Apple Under High Density Plantation
    (Dr Yashwant Singh Parmar University of Horticulture and Forestry;Solan, 2004) Sanjeev Kumar; Rehalis, A.S.