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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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2013 - 20192
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Author: RANA, SABINA × Type: Thesis ×
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    ThesisItemOpen Access
    IN VITRO SELECTION AND REGENERATION OF SALIX SP. AGAINST CANKER DISEASE
    (UHF,NAUNI, 2019-05) RANA, SABINA; THAKUR, SANJEEV
    ABSTRACT In the present investigations,in vitro regeneration of commercially importantSalix sp. was carried out and an attempt was made for in vitro selection of this species against Cytospora along with molecular studies. Indirect organogenesis form leaf and inter nodal segment was achieved. Treatment of 12.5% NaOCl for 10 minutes to both explants along with 0.2% bavistin was found to be the best as it gave maximum number of uncontaminated cultures and per cent survival. Callus was induced from leaf and inter nodal explant on MS medium supplemented with 0.5 mg/l NAA with 1.5 mg/l BA and 0.5 mg/l NAA with 0.5 mg/l BA respectively. Highest percentage of callus was obtained from leaf (96.67%) explants followed by inter nodal segment (93.34%). Percent shoot induction from leaf and inter nodal segment derived calli on 0.5 mg/l BA, 0.50 mg/l Kinetin with 0.25 mg/l GA3 and 0.5 mg/l BA with 0.25 mg/l GA3 was 83.33% and 75.00% respectively. Leaf explants was found to be most responsive explants for indirect regeneration. Highest multiplication rate (1:4) was obtained on MS medium fortified with 1.50 mg/l BA with 0.25 mg/l GA3. Shoot number was elevated with the increase in subculturing passages upto third passage thereafter there had been a decline in it. Activated charcoal brings about elongation of shoots. The regenerated shoots were rooted in half strength MS medium containing 500 mg/l activated charcoal. During hardening it was observed that plantlets showed highest survival of 30% in a potting mixture containing sand, soil and cocopeat. To carry out in vitro selection for resistance development, the isolation of canker causing pathogen was done. On the basis of morphological features and BLASTn analysis of ITS region of pathogen, it was identified as Cytospora chrysosperma. The optimum concentration of fungal culture filtrate for selection against canker disease was 30.00 per cent resulting in 12.96 per cent survival of calli. However no shoots were regenerated after selection of calli. For molecular characterization dendrograms were generated to assess the variations among mother plant, control calli and 30 per cent FCF treated calli, using RAPD and ISSR markers which separated them in two clusters where mother plant and control plant always fall in one cluster showing 70 and 79 percent similarity with each other while the selected variants clustered randomly suggesting genetic variation with mother plant, control plant and within the variants.
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    ThesisItemOpen Access
    ASSESSMENT OF GENETIC DIVERSITY IN Aloe vera L. AMONG DIFFERENT PROVINCES OF H.P.
    (2013) RANA, SABINA; KANWAR, KAMLESH
    ABSTRACT The present investigation “Assessment of genetic diversity in Aloe vera L. among different provinces of H.P.” was undertaken using morphological, biochemical, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. On the basis of morphological and biochemical characters very low variation was found among selected genotypes. A total of 30 RAPD primers and 6 ISSR primers were screened and out of 30 only 24 RAPD and all the 6 ISSR primers gave amplification. In RAPD analyses, a total of 91 bands were amplified, out of which 82 were polymorphic in nature however in case of ISSR markers, 21 polymorphic bands were observed from a total of 24 bands. The amplified products from both the analysis ranged from 100 to 3000 bp and 250 to 1500 bp respectively. Maximum number of bands 107 was scored with primer OPE-10 during RAPD analysis and 114 bands with primer hb-13 during ISSR studies. The similarity coefficient value ranged from 0.62 to 0.91 on the basis of dendrogram. Cluster analysis, using UPGMA, SAHN clustering (NTSYS-pc ver. 2.0) grouped the genotypes into 4 main clusters. C1 genotype showed its distinct nature by sidelining at 0.62 similarity index value leaving behind 65-91% genetic similarity among the remaining genotypes. On the basis of dendrogram formed by ISSR markers 57-100% of genetic similarity was obtained forming two clusters representing 3 and 21 genotypes in each, thereby depicting 80% similarity in minor and 65% similarity in major cluster. Hence the study showed existence of genetic relatedness within the species.