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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Author: GUPTA, ANUPAMA × End date: 2017 × Start date: 2012 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    CHARACTERIZATION OF POTENTIALLY ACTIVE NEW PROBIOTIC STRAINSISOLATEDFROM DIFFERENT SOURCES AND TO STUDY THEIR PROSPECTS AS NUTRACEUTICAL AGENTS
    (2015) GUPTA, ANUPAMA; SHARMA, NIVEDITA
    ABSTRACT The present investigation was carried out to isolate most efficient potential probiotic lactic acid bacteria from rare and unexplored food sources, their screening, identification, safety assessment, evaluationof probiotic attributes, metabolic fingerprinting as well the development of novel nutraceutical products. In total 27 bacterial isolates were obtained from fermented/non fermented food sources. Out of all, 6 bacterial isolates were screened by bit/disc as well as well diffusion method on the basis of their wide inhibitory spectrum against tested pathogens. Isolates C-1, LB-CC, LB-WC, Ch-1, Ch-2 and 107 were selected for further studies having widest antagonistic potential, which were isolated fromkodo millet flour, lasoda bari, chuli and salori, respectively that are traditional food products of Himachal Pradesh. Isolates C-1, LB-CC and LB-WC were identifies as Pediococcus pentosaceus, Ch-1 as Enterococcus faecium, Ch-2 as Pediococcus acidilactici while 107 identified as Pediococcus damnosus by 16S rRNA gene technique and registered in NCBI under accession no. KM251461, KM251460, KJ489415, KJ541885, KJ541886 and KJ577588, respectively. Safety assessment of isolates was done by evaluating antibiotic susceptibility, haemolytic, DNase and gelatinase activities. All the isolates exhibited 80-100% antibiotic sensitivity, non-heamolytic, non-DNase and non-gelatinase activities, thereby proving their safe status. These screened isolates were further examined for their probiotic potential viz. acidity tolerance, bile tolerance, simulated gastrointestinal transit, auto-, co-aggregation, hydrophobicity, adhesion to gastric as well as mammalian cell lines, competitive exclusion of pathogens, antimicrobial potential, exopolysaccharides and β-galactosidase production, compatibility and cumulative probiotic potential. All the six screened isolates were found to be highly acidity tolerant strains with 93.65-99.41 % survival rate at pH 3 for 3 h. These six isolates were able to resist high bile salt concentration i.e. 2.0% with 93.12-98.22 % survival rate for 8 h. Resistance to gastrointestinal transit as found to be in a range from 43.09-90.12 %. All the six isolates exhibited good autoaggregation capacity i.e. greater than 40% after 5 h and showed string hydrophobicity towards xylene i.e. > 40%. All the isolates were able to adhere to gastric mucin with 14.7-49.5% adhesion and all showed adhesion potential to mammalian epithelial cell lines. These screened probiotic isolates showed broad and strong inhibitory spectrum against both gram positive as well as gram negative pathogenic bacteria through secretion of antimicrobial metabolites viz. lactic acid (7.18-8.46 mg/l), H2O2 (0.24-0.38g/l) and bacteriocins (77.33-100.0 AU/ml). Metabolic fingerprinting was done to elucidate the total metabolic profile of isolates and P. pentosaceus LB-CC and P. acidilactici Ch-2 were being first lactic acid bacteria to be reported for the production of squalene with anticancerous properties. The entire screened six isolates were highly qualified with cumulative score of 95.83% and are being used to prepare probiotic nutraceutical products with high antioxidant content viz. novel cereal based probiotic acidulant, probiotic enriched mango fortified shrikhand and probiotic enriched pulse fortified yogurtand are successfully accepted in their sensory evaluation. Hence, this study affirms the use of P. pentosaceus C-1, P. pentosaceus LB-CC, P. pentosaceus LB-WC, E. faecium Ch-1, P. acidilactici Ch-2 and P. damnosus 107 in the development of new functional foods and nutraceutical preparations to impart betterment to the public health as these six strains isolated in the present study have been proved safe as well as highly effective probiotic candidates
  • Thumbnail Image
    ThesisItemOpen Access
    CHARACTERIZATION OF POTENTIALLY ACTIVE NEW PROBIOTIC STRAINS ISOLATED FROM DIFFERENT SOURCES AND TO STUDY THEIR PROSPECTS AS NUTRACEUTICAL AGENTS
    (2015) GUPTA, ANUPAMA; SHARMA, NIVEDITA
    ABSTRACT The present investigation was carried out to isolate most efficient potential probiotic lactic acid bacteria from rare and unexplored food sources, their screening, identification, safety assessment, evaluation of probiotic attributes, metabolic fingerprinting as well the development of novel nutraceutical products. In total 27 bacterial isolates were obtained from fermented/non fermented food sources. Out of all, 6 bacterial isolates were screened by bit/disc as well as well diffusion method on the basis of their wide inhibitory spectrum against tested pathogens. Isolates C-1, LB-CC, LB-WC, Ch-1, Ch-2 and 107 were selected for further studies having widest antagonistic potential, which were isolated from kodo millet flour, lasoda bari, chuli and salori, respectively that are traditional food products of Himachal Pradesh. Isolates C-1, LB-CC and LB-WC were identifies as Pediococcus pentosaceus, Ch-1 as Enterococcus faecium, Ch-2 as Pediococcus acidilactici while 107 identified as Pediococcus damnosus by 16S rRNA gene technique and registered in NCBI under accession no. KM251461, KM251460, KJ489415, KJ541885, KJ541886 and KJ577588, respectively. Safety assessment of isolates was done by evaluating antibiotic susceptibility, haemolytic, DNase and gelatinase activities. All the isolates exhibited 80-100% antibiotic sensitivity, non-heamolytic, non-DNase and non-gelatinase activities, thereby proving their safe status. These screened isolates were further examined for their probiotic potential viz. acidity tolerance, bile tolerance, simulated gastrointestinal transit, auto-, co-aggregation, hydrophobicity, adhesion to gastric as well as mammalian cell lines, competitive exclusion of pathogens, antimicrobial potential, exopolysaccharides and β-galactosidase production, compatibility and cumulative probiotic potential. All the six screened isolates were found to be highly acidity tolerant strains with 93.65-99.41 % survival rate at pH 3 for 3 h. These six isolates were able to resist high bile salt concentration i.e. 2.0% with 93.12-98.22 % survival rate for 8 h. Resistance to gastrointestinal transit as found to be in a range from 43.09-90.12 %. All the six isolates exhibited good autoaggregation capacity i.e. greater than 40% after 5 h and showed string hydrophobicity towards xylene i.e. > 40%. All the isolates were able to adhere to gastric mucin with 14.7-49.5% adhesion and all showed adhesion potential to mammalian epithelial cell lines. These screened probiotic isolates showed broad and strong inhibitory spectrum against both gram positive as well as gram negative pathogenic bacteria through secretion of antimicrobial metabolites viz. lactic acid (7.18-8.46 mg/l), H2O2 (0.24-0.38g/l) and bacteriocins (77.33-100.0 AU/ml). Metabolic fingerprinting was done to elucidate the total metabolic profile of isolates and P. pentosaceus LB-CC and P. acidilactici Ch-2 were being first lactic acid bacteria to be reported for the production of squalene with anticancerous properties. The entire screened six isolates were highly qualified with cumulative score of 95.83% and are being used to prepare probiotic nutraceutical products with high antioxidant content viz. novel cereal based probiotic acidulant, probiotic enriched mango fortified shrikhand and probiotic enriched pulse fortified yogurt and are successfully accepted in their sensory evaluation. Hence, this study affirms the use of P. pentosaceus C-1, P. pentosaceus LB-CC, P. pentosaceus LB-WC, E. faecium Ch-1, P. acidilactici Ch-2 and P. damnosus 107 in the development of new functional foods and nutraceutical preparations to impart betterment to the public health as these six strains isolated in the present study have been proved safe as well as highly effective probiotic candidates.