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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Plant Pathology × Author: BRAKTA, AJAY × Start date: 2010 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF APPLE STEM PITTING VIRUS
    (2015) BRAKTA, AJAY; THAKUR, P.D.
    ABSTRACT Surveys conducted during 2011 and 2012 in different apple growing areas of Himachal Pradesh, Uttarakhand and Jammu and Kashmir revealed viral disease incidence ranging from 3 to 60 per cent. Chlorotic spots coupled with necrotic lesions on apple leaves were the predominant symptoms. Orchards located at Regional Horticulture Research Station, Mashobra and Dhangvi village of Kotkhai area were selected for conducting biological and serological detection of apple stem pitting virus (ASPV). Serological detection through DAC and DAS-ELISA resulted in the detection of apple stem pitting virus (ASPV) either alone or in mixed infection with apple chlorotic leaf spot virus (ACLSV), apple mosaic virus (ApMV) and apple stem grooving virus (ASGV). Biological detection of one of the ASPV seropositive isolate on herbaceous hosts resulted in the production of symptoms on Chenopodium quinoa, C. amaranticolor, Nicotiana tabacum var. White Burley and Phaseolus vulgaris. Detection on woody indicators (Malus pumila Spy 227 and Jay Darling) under field conditions through double grafting of inoculators and indicator budwood resulted in the production of typical viral symptoms on leaves in Jay Darling and Spy 227indicators. Graft incompatibility and necrotic symptoms were produced at the graft union of Spy 227 indicator followed by decline and dieback. Leaf samples drawn during March to May months were found suitable for the ELISA of ASPV whereas petals were the best source in addition to seropositive detection of ASPV in anthers and sepals. Association of ASPV with viral symptoms in apple was also confirmed by RT-PCR assays. Internal control primers along with coat protein gene specific primers were used to overcome the problem of false negative results. Molecular characterization of full coat protein gene of 3 ASPV isolates, 5 ASGV isolates and partial characterization of coat protein gene of 10 ACLSV isolates was carried out and the resultant sequences were then submitted to NCBI. Phylogenetic analysis of sequences showed the presence of variability among isolates and confirmed that there is no correlation between the geographic origin and genetic diversity of these isolates, which does not allow drawing conclusion on their origin and dispersion. Serological indexing resulted in the selection of 13 trees of 7 cultivars free from infection of ASGV, ACLSV, ASPV and ApMV in ELISA test of 36 symptomless trees of 12 cultivars