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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Molecular Biology and Biotechnology × Author: SHARMA, POOJA × End date: 2019 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    PLANT REGENERATION, GENETIC TRANSFORMATION AND EXPRESSION OF FOREIGN GENE IN LETTUCE (Lactuca sativa L.)
    (2013) SHARMA, POOJA; SRIVASTAVA, D.K.
    ABSTRACT The investigation was carried out to standardize a protocol for plant regeneration, genetic transformation and expression of foreign gene in lettuce (Lactuca sativa L.). Plant regeneration studies were carried out using two types of explants viz leaf and petiole. The leaf explants shows high percentage of shoot regeneration (82.44%) on MS medium supplemented with 0.25mg/l BAP and 0.10mg/l NAA as compared to petiole explants (64.18%) on MS medium supplemented with 0.50 mg/l kinetin + 0.25mg/l NAA. MS medium supplemented with 0.50mg/l IBA was found to be best for root regeneration (81.43%). For genetic transformation, disarmed Agrobacterium tumefaciens LBA 4404 strain containing a reporter β- glucuronidase [uid A (gus)] gene in binary vector (pBI 121) system along with kanamycin resistance gene (npt-II) for selection in both bacteria and plant was used for co-cultivation experiment to transfer uid A (gus) and npt-II genes in lettuce. After co-cultivation only the transformed cells/ explants were able to grow on selective shoot regeneration medium (50mg/l Kanamycin and 500mg/l cefotaxime) whereas non-transformed cells/ explants turned brown and died on the selective medium. Transformation experiment could be scored as early as 3-4 weeks after selection. The effect of pre-culturing and co-cultivation was studied. Explants pre-cultured for 72 hours and co-cultivated for 48 hours resulted in improved transformation frequency. Putative transformed calli and shoot were obtained from leaf explants, which were able to grow on the selective medium containing 50mg/l Kanamycin. The presence of npt-II and uid A (gus) was confirmed by PCR using designed primers. Out of 8 putative transformed calli 5 have shown the amplification of npt-II and 4 shown the amplification of uid A (gus) genes. Expression of uid A (gus) gene was studied in these transformed calli by GUS assay using biochemical and histochemical methods. Five transformed calli which showed presence of npt-II and four transformed calli which showed presence uid A (gus) genes were also GUS positive. A protocol for plant regeneration and genetic transformation in lettuce tissues has been standardized.