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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Molecular Biology and Biotechnology × Author: SHARMA, DIVYA × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON CHARACTERIZATION OF TRYPSIN INHIBITOR FROM Dolichos biflorus
    (2014) SHARMA, DIVYA; NATH, AMARJIT K.
    ABSTRACT Protease inhibitors present in seeds of leguminous plants possess strong inhibitory activity against trypsin and show resistance against pests. Trypsin inhibitor from seed flour of Dolichos biflorus L. showed highest activity in distilled water (pH 7.0) in 4 hours of extraction time. Among the three cultivars of Dolichos biflorus L. HPK-4 cultivar was found to have maximum total and specific inhibitor activity. In developing seeds (7 to 70 DAF) inhibitor activity increased with seed maturation. After six months of storage at room temperature there was significant decline in trypsin inhibitor activity and seeds were infested with beetles but seeds stored at 4oC showed no decline in inhibitor activity. The inhibitor activity in HPK-4 cultivar was found to decrease during germination period (1-11 days). Trypsin inhibitor was partially purified from seed flour extract of HPK-4 cultivar to 4.78 fold with 72.89 per cent recovery by ammonium sulfate precipitation and gel filtration chromatography on Sephadex G-10 and G-100 column. Linear increase in percent inhibition with increase in inhibitor concentration was observed till 71%.The partially purified inhibitor was found to be heat stable and retained 98.98% activity of control at 100oC. It was found to have pH optimum of 7.0 and it exhibited non-competitive inhibition pattern. The inhibitor lost its activity on incubation with varying concentrations of DTT there by indicating the role of disulfide linkages in maintaining its stability and three dimensional structures. Pieris brassicae eggs treated with partially purified inhibitor showed 93.4 percent reduction in hatching as compared to control. Feeding bioassay of Pieris brassicae larvae on leaf discs of cabbage coated with partially purified inhibitor showed decline in per cent survival of larvae with increase in inhibitor concentration. Blood clotting time was found to increase by 33 minutes in human blood treated with partially purified inhibitor (200µg) as compared to control. Partially purified protein (120 µg) showed inhibition of fungus Aspergillus versicolor growth in PDA (potato dextrose agar) medium.