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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Molecular Biology and Biotechnology × Author: SHARMA, ANKITA × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON PROTEASE INHIBITOR IN GERMINATED HORSEGRAM (Dolichos biflorus L.) SEEDS
    (2015) SHARMA, ANKITA; NATH, AMARJEET K.
    ABSTRACT Protease inhibitors present in seeds of leguminous plants possess strong inhibitory activity against trypsin and confer resistance against pests and microbes. Trypsin inhibitor from cotyledons of 24 hours soaked seeds of Dolichos biflorus L. cultivar HPK5 showed highest activity in distilled water (pH 7.0) in 3 hours of extraction time. The inhibitor activity was found to decrease gradually with increase in germination period. Decrease in the intensity and number of bands on native PAGE at different stages of seed germination indicated the reduction of soluble protein content with increasing germination period. Partially purified inhibitor protein from Sephadex G-50 was characterized. Linear increase in per cent inhibition with increase in inhibitor concentration was observed till 72 %. The inhibitor protein was found to be thermostable and retained 98 % activity of control at 100 оC. It had pH optima of 7.5. The inhibitor lost its activity on incubation with varying concentrations of DTT thereby indicating the role of disulfide linkages in maintaining its stability and three dimensional structures. Trypsin inhibitor was further partially purified to 3.83 fold with 78.5 per cent recovery by ammonium sulfate precipitation and gel filtration chromatography on Sephadex G-100 column. It exhibited competitive inhibition pattern and showed stability on incubation with urea even at high temperature (97 оC). However, it lost its stability on incubation with 0.6 N HCl at 37 оC and 97 оC. Partially purified inhibitor protein (6.17mg) inhibited the growth of fungus Alternaria spp. to 70 %. It also inhibited the growth of bacterium Ralstonia solanacerum. This study signifies the antimicrobial potential of Dolichos biflorus L. cultivar HPK5 which might be exploited for crop protection programmes.