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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20151
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Subject: Molecular Biology and Biotechnology × Author: Nisha × End date: 2015 × Start date: 2015 × Type: Thesis ×
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    ThesisItemOpen Access
    Studies on production and characterization of tannase (Tannin acyl hydrolase)
    (YSPU, 2015) Nisha; Nath, A.K.
    Penicillium crustosum AN3 isolated from apple orchard soil is the first report from Penicillium genera to produce maximum tannase which is an important industrial enzyme used in biodegradation and food industry. A total of eleven fungal isolates were obtained from apple orchard soil, pine forest soil and botanical garden soil, respectively. Out of these eleven isolates five isolates viz., three isolates from apple orchard soil, one isolate from botanical garden soil and one isolate from pine forest soil were showed zone of degradation on Czapdox minimal medium supplemented with 0.5% tannic acid confirming tannase activity. These isolates were rescreened qualitatively for maximum tannase production in submerged fermentation isolate A3 exhibited maximum tannase activity of 2.48Uml-1. Molecular characterization of these isolates was carried out using 18S rrna gene technology and in silico analysis of 18S rrna gene sequences lead to identification of these fungal isolates as Fusarium redolens AN1, Aspergillus fumigatus AN2, Penicillium crustosum AN3, P.restrictum AN4 and P. commune AN5. Tannase producing bacterial isolates were also obtained on nutrient agar (NA) medium supplemented with 0.5% tannic acid. A total of four bacterial isolates from tea garden soil, pine forest soil, ruminial fluid and sheep excreta were screened on the basis of their ability to utilize tannic acid and were characterized morphologically, biochemically and for tannase activity leads to identification as Klebsiella sp, Enterobacter sp, Staphylococcus sp and E.coli respectively. Molecular characterization two bacterial isolates was carried out using 16S rrna gene technology and in silico analysis of 16S rrna gene sequences lead to confirmation of these isolates as Klebsiella variicola AN1 and Enterobacter hormaechei AN2. Penicillium crustosum AN3 produced maximum tannase (2.45Uml-1) in submerged fermentation was selected for tannase production in SSF. The enzyme was partially purified from culture extract of enzyme. Cultural conditions and process parameters i.e. type of substrate, incubation time, temperature, initial pH, moisture level, substrate concentration, tannic acid concentration, carbon sources, peptone, yeast extract, urea, NH4NO3 were optimized using one factor at a time approach and activity obtained was 39.5Ug-1. Further optimization was carried out using central composite design following response surface methodology with three independent variables which resulted in increased in tannase production to 55.55 Ug-1. The enzyme was partially purified by acetone precipitation and gel filteration chromatography which showed 46.48 yield with 3.94 fold purification and had a molecular mass of 205kDa. Gallic acid the hydrolytic product in crude enzyme was detected by TLC, FTIR and HPLC using gallic acid as standard. Crude enzyme obtained was studied for its ability in pine needle degradation, tea colour decolourization, dye decolourization and removal of apple browning.