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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Molecular Biology and Biotechnology × Author: ASHU, RANI × Start date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDIES ON CHARACTERIZATION AND PURIFICATION OF α-AMYLASE INHIBITOR FROM BEAN (Phaseolus vulgaris L .) CULTIVAR
    (2013) ASHU, RANI; NATH, AMARJEET K.
    ABSTRACT Seven bean (Phaseolus vulgaris L.) cultivars of Himalayan region were analyzed for α-amylase inhibitor activity. α-Amylase inhibitor from seed flour of Phaseolus vulgaris L. showed highest activity in 0.1 M phosphate buffer (pH 7.6) contaning 0.15 NaCl in 1 hours of extraction time. Among the seven cultivars of Phaseolus vulgaris L. Triloki cultivar was found to have maximum total and specific inhibitor activity. The α–amylase inhibitor from seeds of Triloki cultivar was partially purified to 15.25 fold. The specific inhibitor activity increased from 0.19 in crude extract to 3.01 in Gel filtration (Sephadex G-100) chromatography. A single band of the purified inhibitor was obtained by Native–PAGE. SDS-PAGE revealed the purified inhibitor to be a monomer with molecular weight of 25,000 daltons. The nature of inhibition was found to be of non-competitive type as determined Dixon’s plot. The inhibitor was found to be heat labile was stable up to 30°C and retained 55.51 percent activity at 70°C temperature. Inhibitor was found to have two pH optima of 5 and 7.6. The purified inhibitor was found to have inhibitory activity against α–amylase extracted from larvae of Corcyra cephalonica. 100 percent larval mortality of Corcyra cephalonica was observed after 11 days when they were fed on wheat flour mixed with 252 µg of partially purified α- amylase inhibitor. Partially purified α–amylase inhibitor was also found to inhibit the activity of α-amylases of Corcyra cephalonica larvae, Aspergillus oryzae, Human salivary and Bacillus spp. Molecular characterization of bean cultivar Triloki was done by amplification of its genomic DNA using primer OPS-02. The number of bands amplified was 2.