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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Horticulture × Author: SHARMA, ANKITA × End date: 2019 × Start date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    BIOPROSPECTING OF THERMOTOLERANT BACTERIA FROM HOT WATER SPRINGS OF HIMACHAL PRADESH FOR PRODUCTION OF THERMOSTABLE LIPASE ENZYME
    (2013) SHARMA, ANKITA; SHIRKOT, POONAM
    ABSTRACT Thermophiles are the organisms which are adapted to live at high temperatures. The survival of these organisms at high temperature is possible due to the thermostability of their enzymes. Many thermostable enzymes such as Taq DNA polymerase, aldolase, amylase, lipase, protease, cellulase, RNA polymerase etc. find a number of commercial applications because of their thermostability. Therefore, the isolation of thermophilic bacteria from natural sources and their identification is very important in terms of discovering new industrial enzymes. Keeping in view, hot water springs of Himachal Pradesh could serve as a good source for new thermophilic microorganisms with novel industrially important properties. Therefore aim of the present study was the isolation and characterization of thermotolerant bacteria from hot water springs of Himachal Pradesh for production of thermostable lipase enzyme. Four hot water springs viz., Manikaran, Vashisht, Khirganga and Tattapani were purposely selected for the present studies. Samples consisting of soil, water, rock matting and pebbles were collected from each hot water spring. The pH and temperature of the four thermal springs were ranged from 4.1-6.0 and 51-105oC respectively. The chloride, sulphate, total hardness, calcium hardness, CaCO3 hardness and magnesium content ranged from 490-2673, 12-50, 147-483, 30.0-144.2, 65-264 and 7.53-26.73 mg/l respectively. Forty four bacterial isolates were isolated from the samples using tributyrin medium. All the bacterial isolates were studied for various morphological characters and on the basis of ability of formation of zone of clearance on tributyrin medium, forty two bacterial isolates were selected, which were further investigated for biochemical characters. Quantitative screening was done to select one bacterial isolate showing maximum thermostable lipase activity and MBW2 bacterial isolate was found to show maximum thermostable lipase activity of 4.83 U/ml and was thus selected further for molecular characterization. Genomic DNA was isolated from the selected bacterial isolate MBW2. PCR amplification of the isolated DNA was carried out using universal primers for 16S rDNA gene and an amplicon of 1250 bp was obtained. Sequencing of the PCR product was done using same primers. Sequence of the MBW2 bacterial isolate so obtained was found to be 769 bp. In silico analysis using BLASTn showed 99% homology of the query sequence with Aneurinibacillus thermoaerophilus strain L420-91. A total of 25 sequences were mined and these sequences were used to compare the 16S rDNA sequence of the test isolate MBW2. Alignment score was highest for Aneurinibacillus thermoaerophilus strain L420-91 16S ribosomal RNA, partial sequence (NR_029303.1). Phylogenetic analysis based on nucleotide sequences using NJ method was achieved via phylip 3.68 and EXOMETM HORIZON. Aneurinibacillus thermoaerophilus strain MBW2 was studied further for enzymatic studies. Optimization of culture conditions for growth and thermostable lipase activity of isolate Aneurinibacillus thermoaerophilus strain MBW2 was carried out. The crude extracellular extract was produced using optimized conditions and further partially purified using ammonium sulphate precipitation technique. The procedure yielded 32.5 g protein with 1.17 fold purification with a percent yield 53.02%. Molecular weight of the partially purified enzyme was found to be 42.5 kDa using SDS-PAGE gel.