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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Forest Products × Author: SHARMA, SEEMA × End date: 2017 × Start date: 2012 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    STUDIES ON THE COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS AND STANDARDIZATION OF HPLC CONDITIONS FOR ESTIMATION OF ACTIVE CONTENTS IN SOME COMMERCIALLY IMPORTANT MEDICINAL PLANTS
    (UHF,NAUNI, 2017) SHARMA, SEEMA; SHARMA, YASH PAL
    ABSTRACT The present investigation, “Studies on the comparative evaluation of different extraction methods and standardization of HPLC conditions for estimation of active contents in some commercially important medicinal plants” was carried out in the department of Forest Products, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, (H.P.) during 2013-2016. The study was conducted on five important medicinal crops viz., Swertia chirayita, Podophyllum hexandrum, Picrorhiza kurroa, Gloriosa superba and Andrographis paniculata with the objectives as (i.) Standardization of extraction technique, (ii.) Development and validation of HPLC method for quantification of active phytoconstituent(s). For standardization of extraction technique in each crop five different extraction methods viz., soxhlet extraction, reflux extraction, cold extraction, sonication assisted extraction and microwave assisted extraction were used with two different solvents. The extracting solvents and extraction durations vary with conditions. In Swertia chirayita maximum amaroswerin (0.242%) and amarogentin (0.294%) content with higher total extract (17.756%) were obtained under soxhlet extraction for eight hours with methanol solvent. In Podophyllum hexandrum, reflux extraction for four hours with methanol solvent is the best extraction method for extraction of maximum (5.794%) podophyllotoxin content with higher total extract (38.826%). Cold extraction with continous shaking at room temperature with methanol for twelve hours is the best extraction method for extraction of maximum picroside-I (8.600%) and picroside-II (3.834%) with total extract (39.430%) in Picrorhiza kurroa. In Gloriosa superba, maximum colchicine content and total extract was obtained under soxhlet extraction for one hour with methanol solvent or reflux extraction with ethyl alcohol for one hour. For Andrographis paniculata, reflux extraction for one hour with methanol solvent has been found best extraction method for obtaining maximum (2.040%) andrographolide content with total extract (15.111%). Simple and easy to handle HPLC methods were developed and validated for quantification of major phytoconstituents in medicinal crops under study. Each method was validated as per ICH guidelines for seven parameters viz., linearity, range, accuracy, precision, LOD, LOQ and robustness.