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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Biotechnology and molecular Biology × Start date: 2020 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    IN VITRO PROPAGATION AND SECONDARY METABOLITE PRODUCTION OF Angelica glauca EDGEW. - AN ENDANGERED MEDICINAL PLANT OF HIMALAYAS
    (UHF Nauni, 2024-03-26) DEEPIKA NEGI; Manisha Thakur
    The present study reports an optimized protocol for high frequency in vitro propagation through seeds and rhizome buds, secondary metabolite production and molecular analysis in Angelica glauca. Seeds were subjected to different pre-treatments prior to surface sterilization for breaking recalcitrance. Maximum percent survival of seeds (85.19%) was achieved on treatment with 0.1% HgCl2 for 3 minutes with maximum in vitro seed germination and culture establishment (87%) during spring, followed by winter season (65%). The proliferated shoots showed highest multiplication (1:12) with rooting on half strength MS medium fortified with 1.0 mg/l BA + 0.2 mg/l NAA. In rhizome buds highest percent survival of 91.67 and 87.50% was achieved in 0.3-0.5 cm and 1.0-2.0 cm sized buds after surface sterilization with 0.1% HgCl2, for 2 and 3 minutes, respectively. Maximum in vitro establishment (94.44%) was achieved on MS medium fortified with 0.3 mg/l BA and 0.1 mg/l GA3 with 90% success during spring, followed by 78% in winter season. Highest multiplication rate (1:15) with rooting was achieved on MS medium fortified with 1.0 mg/l BA +0.2 mg/l NAA. Endophytic bacterial contamination could be observed in some cultures during multiplication stage after seven to eight months which was uncontrollable and led to the mortality of cultures. The bacterium responsible for it was identified as Agrobacterium pusense AG1, through morphological, biochemical and molecular characterization. For callus induction in vivo leaves and roots were used as explants wherein, highest callus induction in in vivo leaves (88.89%) and roots (80.59%) was observed under dark incubation. During secondary metabolite production, GC-MS analysis revealed maximum (8.79%) production of 1-Monolinoleoylglycerol trimethylsilyl ether from root callus and 6.72% from leaf callus. For enhancing the in vitro yield of bioactive compounds, callus induced from in vivo leaves and roots were subjected to elicitation by incorporating different concentrations of sucrose (1,3 and 5%), methyl jasmonate (0.5-1.5 mM), 2,4D (2.0 mg/l) and Kin (0.5 mg/l) in production medium. Elicitation with 1% sucrose showed maximum Stigmasterol (37.33%) from leaf calli whereas, maximum Oleic acid (34.33%) was monitored through root calli. Sucrose (3%) resulted in production of Stigmasterol (55.85%) from leaf and Erucic acid (21.03%) from root callus, and at 5% concentration 17- Pentatriacontene (25.71%) in leaf callus and 2-Pentanone, 4-hydroxy-4-methyl- (15.09%) in root callus were identified. Addition of methyl jasmonate (0.5 mM) resulted in production of Furfural (15.54%) from leaf callus and trans-13-Octadecenoic acid (31.88%) from root callus, and at 1.0 mM concentration 2- Furancarboxaldehyde, 5-methyl- (22.53%) and trans-13-Octadecenoic acid (33.39%) were monitored from leaf and root callus respectively. On increasing the concentration of methyl jasmonate to 1.5 mM, n-Hexadecanoic acid (16.31%) and cis-13-Octadecenoic acid (27.82%) were identified from leaf and root callus. Addition of 2,4D in the medium resulted in production of 2-Furancarboxaldehyde, 5-methyl- (5.74%) and Furfural (10.62%) from leaf and root calli, respectively. Whereas, addition of kinetin led to the accumulation of 1- Monolinoleoylglycerol trimethylsilyl ether (16.93%) from leaf and (33.51%) from root callus in production medium. Molecular analysis of samples from different altitudes through SCoT and CBDP markers revealed 75% and 80.57% polymorphism, respectively suggesting genetic variations in samples.