Thumbnail Image

Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

Browse

20241
Reset filters
Subject: Biotechnology and molecular Biology × Author: Akash × Type: Thesis × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Purification and characterisation of Trypsin inhibitor from germinating seeds of Leucaena leucocephala L.
    (College of Horticulture and Forestry Dr YSP UHF, Neri, Hamirpur(H.P.), 2024-06-05) Akash; Reena Kumari
    The present investigation "Purification and Characterization of Trypsin Inhibitor from germinating seeds of Leucaena leucocephala L." was done using ammonium sulphate precipitation and ion exchange chromatography on DEAE-Cellulose 52. Seven samples viz., Sl to S7 of Leucaena leucocephala were screened for the presence of trypsin inhibitor. Among all samples, S1 showed maximum trypsin inhibitor activity and S7 showed minimum trypsin inhibitor activity. The results confirmed 21.4% decline in trypsin inhibitor activity on the 5th day of seed germination. Further protein was purified to 5.03, 5.0 and 4.78 fold with a percent yield of 14.52, 14.0 and 13.98 from Sl, S2 and S3, respectively. The molecular weight of purified protein was found to be ~20 kDa. The purified trypsin inhibitor found to be heat stable up to 80℃ and showed resilience in the pH range of 4.0-10. The inhibitor was found to be susceptible to varying concentrations of reducing agents like DTT and β-mercaptoethanol, thereby indicating the role of disulphide bridges in maintaining its three dimensional structure and stability. The purified protein was found to be effective to decrease midgut protease of Pieris rapae. This stability of inhibitor across a broad pHrange (4-10), demonstrates its potential as an insecticidal protein againstLepidopteran and Coleopteran insects.