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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20151
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Subject: Biotechnology and Molecular Biology × Author: SHARMA, SHIKHA × End date: 2015 ×
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    ThesisItemOpen Access
    STUDIES ON GENETIC STABILITY OF IN VITRO PROPAGATED CHRYSANTHEMUM CULTIVAR “PURNIMA” USING MOLECULAR MARKERS
    (2015) SHARMA, SHIKHA; NATH, AMARJEET K.
    ABSTRACT The present research entitled “Studies on genetic stability of in vitro propagated chrysanthemum cultivar “Purnima” using molecular markers.” was carried out during 20132014 & 2014-2015 in the Department of Biotechnology Nauni, Solan (H.P.) Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.). Axillary buds and leaf segments were used as explants for regeneration of plants. Chrysanthemum plants of the cultivar “Purnima”were regenerated from axillary bud by shoot bud proliferation and by an intermediate callus stage from leaves. The concentration of 2mg/l BAP + 0.5mg/l NAA and 1.5mg/l BAP + 0.25 mg/l NAA were found best for establishment of axillary buds and leaf explant. MS medium supplemented with 0.2mg/l BAP + 0.2mg/l NAA+1mg/l GA3 and 1 mg/l BAP + 0.5 mg/l NAA + 1mg/l GA3 concentration were found best for axillary bud proliferation and callus induction from leaf segments, respectively. MS medium supplemented with 1.0mg/l 2, 4-D +1.0 mg/l kinetin and 2mg/l BAP + 0.25mg/l NAA produced maximum number of shoots formed from axillary buds and from callus cultures, respectively. The rooting ½ strength MS medium containing 0.5mg/l IBA, produced maximum number of roots from both explants. Plant regenerated from axillary buds showed early flowering. The genetic stability between mother plant, plant regenerated from axillary bud and callus culture were carried out by using RAPD markers. Genomic DNA was isolated from young leaves of plants using CTAB method and amplified using 20 random decamer primers out of these only 15 produced polymorphism. Similarity coefficient was calculated by using Dices coefficient ranged from 0.30 to 0.50. Dendrogram was constructed by using UPGMA method for the clustering of mother plant, plants regenerated from axillary bud and callus. Mother plant formed separate cluster while plant regenerated from axillary bud and callus together formed one cluster that further subdivided. From the data obtained during present study it can be concluded that plants produced in vitro either by micropropagation or from callus culture were not genetically similar to mother plant.