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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Biotechnology and Molecular Biology × Author: SAMRITI × Start date: 1994 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    DEVELOPMENT AND CHARACTERIZATION OF ESTSSR MARKERS IN Rubus ellipticus SMITH AND THEIR TRANSFERABILITY IN RELATED SPECIES/GENERA
    (UHF,NAUNI, 2018-03) SAMRITI; KAUR, RRAJINDER
    ABSTRACT Rubus ellipticus Smith. commonly known as ‘Yellow Himalayan raspberry’ is an important member of Rosaceae family with antioxidant and antibacterial properties. In the present study EST-SSR markers were developed for R. ellipticus and were used for polymorphism/transferability studies. EST sequences of R. ellipticus were generated from cDNA clones using Paired-End sequencing method on NextSeq 500. The raw data were assembled using DNASTAR (https://www.dnastar.com/ ) software. A total of 7777 EST sequences for R. ellipticus were obtained. ESTs containing SSR motifs were extracted out using an online tool, MISA (http://pgrc.ipk-gatersleben.de/misa). 704 ESTs were used for SSR extraction. SSR primers were designed from the SSR containing EST sequences using Websat software (http:/www.wsmartins.net/websat) and 68 randomly selected primer pairs were custom synthesized. Polymorphism study was carried out using 68 SSR primer pairs among 21 collections of Rubus ellipticus and 31 members of Rosaceae family. For polymorphism studies, DNA was isolated from young leaves using CTAB method (Doyle and Doyle, 1987). The EST-SSR primers revealed 95.3% and 93.6% polymorphism in diversity and cross transferability analysis. Jaccard’s similarity matrix was developed and dendrograms were generated using NTSYSpc ver.2.02h to establish the percent similarity among the 21 collections of Rubus ellipticus and 31 members of Rosaceae family. With EST-SSR primers, two clusters were obtained. Maximum similarity i.e 69% was found between ‘Kharkog-1’ and ‘Majhgaon’ of Rubus ellipticus and 87 % betweenn‘High and magic’ and ‘Chaubattia ambrose’ of Rosaceae family. Thus EST-SSRs used in the present study revealed a high level of polymorphism in the 21 colllections of Rubus ellipticus and 31 members of Rosaceae family, revealing their efficiency in fingerprinting analysis.