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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20181
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Subject: Biotechnology × Author: SHARMA, POOJA × End date: 2019 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON CHARACTERIZATION OF CARNATION (Dianthus caryophyllus L.)GENOTYPES USING MOLECULAR MARKERS
    (UHF,NAUNI, 2018-08) SHARMA, POOJA; NATH, AMARJIT K
    ABSTRACT Carnation (Dianthus caryophyllus L.) is one of the most remunerative cut flower of the world and belongs to the family caryophyllaceae. In the present study, characterization of carnation genotypes viz., Tempo, Bizet, Gwen, Tasman, White Wedding, Diana Yellow, Jurano, Pirandello, Dona, Hermis, Master, Gaudiana and mutants viz., UHFSCar-1, UHFSCar-2, UHFSCar-3, UHFSCar-4, UHFSCar-5, UHFSCar-6, UHFSCar-7 and UHFSCar-10 were carried out by using Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Simple Sequence Repeats (SSR) markers. Genomic DNA was isolated from tender leaves of carnation using CTAB method (Doyle and Doyle, 1987). Eight RAPD, seven ISSR and seven SSR primers were able to amplify the genomic DNA. Total number of bands amplified in carnation genotypes and mutants using RAPD markers were 71, out of which 67 were polymorphic and 4 were monomorphic. In ISSR studies, total numbers of bands amplified were 99, out of which 96 were polymorphic and 3 were monomorphic. Total number of bands obtained on separation of PCR reaction carried out using SSR markers resolved in agarose gel was 35, out of which 33 were polymorphic and 2 were monomorphic. However, in polyacrylamide gel, total numbers of bands amplified were 41, out of which 39 were polymorphic and 2 were monomorphic. Total numbers of unique bands obtained were 20, 25, 7 and 8 using RAPD, ISSR, SSR markers in agarose and polyacrylamide gel, respectively. Percentage of polymorphism ranged from 70.00 to 94.74 in RAPD studies, 83.33 to 94.44 in ISSR studies, 66.67 to 87.50 in SSR studies with agarose gel and 83.33 to 92.85 in SSR studies with polyacrylamide gel. Polymorphic information content (PIC) value for RAPD primers ranged from 0.39-0.49 and resolving power (Rp) ranged from 24-62. PIC value for ISSR primers ranged from 0.27-0.49 and Rp ranged from 32-70. For SSR markers resolved in agarose gel, PIC value ranged from 0.00-0.63 and Rp ranged from 10-38. However, for SSR markers resolved in polyacrylamide gel, PIC value ranged from 0.000.79 and Rp ranged from 10-66. Jaccard’s similarity matrix was developed and dendrograms were generated using NTSYSpc version 2.20. Similarity index was computed based on Jaccard’s coefficient and used for cluster analysis based on UPGMA. The findings of the present investigations indicated high genetic diversity in carnation genotypes and mutants which could be used in carnation breeding programmes. Unique bands obtained for carnation genotypes and mutants could be used for varietal identification. RAPD, ISSR and SSR markers were found to be effective in assessing genetic diversity studies and relatedness between carnation genotypes and mutants. The unique DNA markers would help the Department of Floriculture and Landscape Architecture in release of carnation mutants as new varieties.