STUDIES ON CHARACTERIZATION OF CARNATION (Dianthus caryophyllus L.)GENOTYPES USING MOLECULAR MARKERS
Loading...
Date
2018-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI
Abstract
ABSTRACT
Carnation (Dianthus caryophyllus L.) is one of the most remunerative cut flower of the world and belongs to the family caryophyllaceae. In the present study, characterization of carnation genotypes viz., Tempo, Bizet, Gwen, Tasman, White Wedding, Diana Yellow, Jurano, Pirandello, Dona, Hermis, Master, Gaudiana and mutants viz., UHFSCar-1, UHFSCar-2, UHFSCar-3, UHFSCar-4, UHFSCar-5, UHFSCar-6, UHFSCar-7 and UHFSCar-10 were carried out by using Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Simple Sequence Repeats (SSR) markers. Genomic DNA was isolated from tender leaves of carnation using CTAB method (Doyle and Doyle, 1987). Eight RAPD, seven ISSR and seven SSR primers were able to amplify the genomic DNA. Total number of bands amplified in carnation genotypes and mutants using RAPD markers were 71, out of which 67 were polymorphic and 4 were monomorphic. In ISSR studies, total numbers of bands amplified were 99, out of which 96 were polymorphic and 3 were monomorphic. Total number of bands obtained on separation of PCR reaction carried out using SSR markers resolved in agarose gel was 35, out of which 33 were polymorphic and 2 were monomorphic. However, in polyacrylamide gel, total numbers of bands amplified were 41, out of which 39 were polymorphic and 2 were monomorphic. Total numbers of unique bands obtained were 20, 25, 7 and 8 using RAPD, ISSR, SSR markers in agarose and polyacrylamide gel, respectively. Percentage of polymorphism ranged from 70.00 to 94.74 in RAPD studies, 83.33 to 94.44 in ISSR studies, 66.67 to 87.50 in SSR studies with agarose gel and 83.33 to 92.85 in SSR studies with polyacrylamide gel. Polymorphic information content (PIC) value for RAPD primers ranged from 0.39-0.49 and resolving power (Rp) ranged from 24-62. PIC value for ISSR primers ranged from 0.27-0.49 and Rp ranged from 32-70. For SSR markers resolved in agarose gel, PIC value ranged from 0.00-0.63 and Rp ranged from 10-38. However, for SSR markers resolved in polyacrylamide gel, PIC value ranged from 0.000.79 and Rp ranged from 10-66. Jaccard’s similarity matrix was developed and dendrograms were generated using NTSYSpc version 2.20. Similarity index was computed based on Jaccard’s coefficient and used for cluster analysis based on UPGMA. The findings of the present investigations indicated high genetic diversity in carnation genotypes and mutants which could be used in carnation breeding programmes. Unique bands obtained for carnation genotypes and mutants could be used for varietal identification. RAPD, ISSR and SSR markers were found to be effective in assessing genetic diversity studies and relatedness between carnation genotypes and mutants. The unique DNA markers would help the Department of Floriculture and Landscape Architecture in release of carnation mutants as new varieties.
Description
Keywords
null