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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20211
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Subject: Biotechnology × Author: SHARMA, NEHA × End date: 2021 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    IN VITRO PROPAGATION AND SECONDARY METABOLITE PRODUCTION IN Podophyllum hexandrum ROYLE (INDIAN MAYAPPLE)
    (UHF,NAUNI, 2021-12) SHARMA, NEHA; THAKUR, MANISHA
    ABSTRACT The present study reports an optimized protocol for high frequency in vitro propagation through seeds and rhizome buds, molecular analysis and podophyllotoxin production in Podophyllum hexandrum. Maximum percent uncontamination of seeds (89%) was achieved on treatment with 0.1% HgCl2 for 3 minutes and highest percent surviving rhizome buds (82.62%) were obtained after surface sterilization with 0.1% HgCl2+1.0% NaOCl for 4 minutes with maximum in vitro establishment (95.97%) on MS medium fortified with 0.2mg/l BA and 0.1mg/l GA3. Maximum in vitro establishment through seeds (89.00%) and buds (90%) was achieved during spring, followed by 68 and 79% in winter season. The proliferated shoots from seeds showed highest multiplication (1:11) on MS medium fortified with 2.0mg/l BA + 0.2mg/l NAA+ 0.7mg/l CaCl2. Addition of CaCl2 (0.7mg/l) into the medium was done to prevent shoot chlorosis. For shoot regenerating from rhizome buds highest multiplication rate of 1:5 was achieved on MS medium fortified with 2.0mg/l BA +0.2mg/l NAA. Molecular analysis was done using SCoT and CBDP markers. SCoT markers showed 71% polymorphism in samples from different altitudes in comparison to 50.90% polymorphism depicted by CBDP markers. However, both the makers showed 100% monomorphism among mother plants and their tissue culture raised progeny. In vivo leaves and petioles as well as in vitro roots and leaves were used as explants for callus induction. Highest callus induction was observed under dark incubation in in vivo leaves (81.07%), petioles (87.19%), in vitro roots (90.67%) and leaves (89.20%).HPLC analysis revealed maximum (0.267%) production of podophyllotoxin from callus initiated from in vitro roots procured from experimental material of district Kinnaur followed by 0.258% from in vitro root callus of Lahaul – Spiti. For enhancing podophyllotoxin yield callus induced from in vitro roots of P. hexandrum plants of district Kullu were subjected to elicitation by incorporating different concentrations (0.5-1.5 mM) of elicitors (methyl jasmonate and chitin) in production medium. Highest podophyllotoxin content (0.33%) was observed after elicitation with 1mM methyl jasmonate followed by its higher concentration of 1.5 mM (0.31%).