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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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2017 - 20192
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Subject: Biotechnology × Author: SHARMA, ANKITA × End date: 2019 × Start date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDIES ON ANNEXIN GENE FAMILY FROM SOLANACEOUS VEGETABLES
    (UHF,NAUNI, 2019-12) SHARMA, ANKITA; NATH, AMARJIT K.
    ABSTRACT Heat stress leads to adverse effect on Potato cultivation affecting the tuberization and the bulking process. Being a signaling molecule, it is hypothesized that annexins might play important role in tuberization under heat stress condition. Ten annexins from potato and 13 annexins from tomato were identified using bioinformatics tool. Annexin gene proteins from potato and tomato were found to have an average molecular weight of 36kDa. Both potato and tomato annexins showed differential location of the annexin genes on 12 chromosomes. Expression profiling of Potato annexin genes under heat stress conditions revealed strong up-regulation of annexins in heat tolerant potato cultivar Kufri Surya as compared to susceptible one i.e Kufri Chandramukhi indicating their role as key signaling molecule under heat stress conditions. The annexin gene StAnn1 showed a uniform pattern of elevated gene expression in both leaves and stolon under heat stress throughout the developmental stage of potato cultivar KS as compared to potato cultivar KCM. Significant increase in peroxidase, anthocyanin and proline content was observed in heat tolerant cultivar Kufri Surya on different intervals of exposure to heat stress. The content of MDA was significantly less in Kufri Surya as compared to Kufri Chandramukhi indicating much less membrane damage in the tolerant potato cultivar KS. The annexin gene StAnn1 was isolated and cloned from Kufri Surya and over expressed in heat susceptible cultivar Kufri Chandramukhi for exploiting its potential for providing heat stress tolerance in other susceptible potato cultivars. Agrobacterium mediated genetic transformation protocol was standardized for potato cultivar Kufri Chandramukhi where selective MS medium with composition containing IAA 0.042mg/L, GA33.0 mg/L, Zeatin 3.0 mg/L, NAA 0.008 mg/L, Kan 50 mg/L, Carb 250 mg/L, Cefo 100 mg/L showed maximum percentage of callus formation i.e 76 % and shoot regeneration. The best rooting was observed on selective root regeneration MS medium containing 50mg/L Kan and 0.3mg/L IAA. On exposure to in vitro salt stress, transgenic KCM plants expressing StAnn1 annexin gene, phenotypically showed better tolerance as compared to the control plants depicting its potential in providing tolerance to other abiotic stress also. A decline in number of roots, nodes/plant and plant height was observed in control shoots as compared to putative transgenic shoots.
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    ThesisItemOpen Access
    BIOPROSPECTING OF SILVER NANOPARTICLES SYNTHESIZING BACTERIA AND POTENTIAL APPLICATIONS
    (UHF,NAUNI, 2017) SHARMA, ANKITA; SHIRKOT, POONAM
    ABSTRACT The synthesis of nanostructured materials, especially metallic nanoparticles, has accrued utmost interest over the past decade owing to their unique properties that make them applicable in different fields of science and technology. The biological method of nanoparticles synthesis is a relatively simple, cheap and environmentally friendly method than the conventional chemical and physical methods of synthesis. Therefore, isolation and identification of bacteria with ability of silver nanoparticles synthesis from natural sources is very important in terms of discovering new industrial products. Keeping in view, a silver mine located at Uchich village of Pulga valley, apple orchards located in Chong village and two hot water springs located in Manikaran and Vashisth in Kullu district of Himachal Pradesh were selected as a source for new silver nanoparticles synthesizing bacteria. Therefore, aim of present study was the isolation and characterization of silver nanoparticles synthesizing bacteria from these sites for biosynthesis of silver nanoparticles followed by applications of these biosynthesized silver nanoparticles to control various plant pathogens, textile dye degradation, phytotoxicity study and pesticide degradation. A total of 106 putative silver nanoparticles synthesizing bacterial isolates were obtained from 45 samples collected from different selected sites. Thirty bacterial isolates were selected on the basis of their ability to show maximum silver nanoparticles synthesizing activity which were characterized morphologically and biochemically. Four bacterial isolates exhibiting maximum silver nanoparticles synthesizing activity viz., UMAS1, UMBS1.1, UMBP1 and UMBP2 were selected for further, molecular characterization using 16S rrna gene technology. In silico analysis of 16S rrna gene sequences led to identification of these bacterial isolates and all the four were found to belong to genus Bacillus and were identified as Bacillus siralis strain UMAS1, Bacillus siralis strain UMBS1.1, Bacillus algicola strain UMBP1 and Bacillus tianmuensis strain UMBP2. On the basis of maximum silver nanoparticles synthesizing activity Bacillus siralis strain UMBS1.1 was selected for in vitro biosynthesis of silver nanoparticles. Maximum silver nanoparticles synthesis was achieved at 60°C, pH: 8.0 and after 24 hrs of incubation with 3.0mM silver nitrate, 3.0 % tryptone, 3.0 % yeast extract and 2.0% inoculum size. Central Composite Design was used to determine the optimal values of incubation time (A), incubation temperature (B), pH (C), tryptone concentration (D) and yeast extract concentration (E). The highest activity was obtained from Run number-22, which consisted of incubation time of 46.0 hrs; incubation temperature of 36.5°C; pH 8.5, 3g/l tryptone and 3g/l yeast extract leading to 16.06 fold increase in silver nanoparticles activity. In vitro biosynthesis of silver nanoparticles was carried out using optimum conditions which were characterized using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-rays diffractions (XRD), Scanning Electron Microscopy (SEM) and Dynamic Light Scattering (DLS). In the present study, ability of silver nanoparticles and bacterial culture preparation of Bacillus siralis strain UMBS1.1 was assessed for degradation of eight textile dyes produced significant results. Both Bacillus siralis strain UMBS1.1 culture preparation as well as silver nanoparticles has significant potential for use in the detoxification of eight textile dyes and for treating textile waste waters including water recycling. Silver nanoparticles and bacterial culture preparation were also found to inhibit various fungal and bacterial pathogens under in vitro conditions appreciably. Silver nanoparticles and Bacillus siralis strain UMBS1.1 culture preparation were also found to degrade chloropyrifos significantly