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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20201
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Subject: Biotechnology × Author: KAPOOR, BHUVNESH × End date: 2024 × Start date: 2020 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    AGROBACTERIUM MEDIATED DEFENSIN GENE TRANSFER STUDIES IN TOMATO (SOLANUM LYCOPERSICUM L.)
    (NAUNI,UHF, 2020-11) KAPOOR, BHUVNESH; SHARMA, RAJNISH
    ABSTRACT Tomato (Solanum lycopersicum L.) is one of the most economically important horticultural crops consumed globally. The crop known as ‘Red Gold’ in the farming community is suffered every year due to number of pathogenic diseases which are country-wide in occurrence. Considering the limitations of conventional methods of crop improvement, present study was taken to develop the transgenic lines of tomato by expressing defensin gene (TvD1) having antimicrobial activity. As a prerequisite to the genetic transformation procedures, regeneration protocol for tomato cv. Solan Lalima was standardized by using different explants. The cotyledon explants exhibited best shoot regeneration response on MS medium augmented with various growth regulators with maximum average number of shoots per explant, highest mean shoot length, minimum average days taken for the initiation of shoot induction, respectively as compared to hypocotyl and leaf explants. The in vitro regenerated shoots were further successfully rooted and hardened. However, cotyledons and hypocotyl explants were found to be physiologically more responsive, hence, were further considered for carrying out genetic transformation experiments. The kanamycin sensitivity experiments established that kanamycin concentrations 50 mg l-1 for cotyledon and 100 mg l-1 for hypocotyl explants were found to be effective for inducing selection pressure. The bacteriostatic antibiotic timentin revealed maximum shoot regeneration response with maximum average per cent shoot regeneration in cotyledons (97.70±1.33) and hypocotyls (87.66±1.33). Agrobacterium cell density of 0.2 at O.D600 and infection time of 2 min was found optimum for sufficient infection. Timentin at concentration of 500 mg l-1 was found efficient in controlling Agrobacterium growth. The 48 hrs of pre-culturing followed by 48 hrs of co-cultivation showed highest 13.33±0.11% and 26.66±0.33% shoot regeneration in cotyledon and hypocotyl explants, respectively. Out of total 15 putative tomato transformants considered for PCR analysis, four showed amplifications of nptII and TvD1 gene. Further, three transformed tomato lines exhibited expression of TvD1 gene through semi-quantitative RT-PCR. Therefore, it was inferred that present investigations would be helpful in developing resistance in this economically important horticultural crop by targeting its various devastating fungal and bacterial pathogens.