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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20201
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Subject: Biotechnology × Author: ABHISHEK, KUMAR × Start date: 2004 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    STUDIES ON RNAi-INDUCED GENE SILENCING BASED RESISTANCE TO MARSSONINA CORONARIA IN APPLE
    (UHF,NAUNI, 2020-08) ABHISHEK, KUMAR; MODGIL, MANJU
    ABSTRACT Marssonina blotch is one of the most devastating diseases caused by Marssonina coronaria (Ellis & J.J. Davis). All the available commercial apple cultivars are found susceptible to this disease. In the present study, we developed an RNAi construct to target HSP90 gene of this fungus for functional analysis. For this, the full stretch of HSP90 cloned in pTZ57R/T was obtained from a recent study in our laboratory. In silco analysed 124 bp off-targeted sequence was cloned into the pGEMT and then cloned in sense antisense orientation into the pMVR-hp vector to develop pMVR-HSP90-RNAi construct. For using the same RNAi construct for fungal transformation, HSP-90 RNAi casstte from pMVR-HSP 90-RNAi vector was transferred to the pCAMBIA1300 vector, which harbours hygromycin B phosphotransferase (hpt) gene as a selection marker. In fungal transformation experiment, no fresh growth was observed in case of mycelial plugs transformed with RNAi construct in comparison to positive control. Agrobacterial overgrowth was found to be a problem after cocuiltivation. Another construct pRI 101-AN-HSP-90RNAi developed in our laboratory was used for transformation of apple cv. Red Chief. MS medium supplemented with 5 mg/l BA and 0.2 mg/l NAA resulted in best shoot regeneration frequency (96.35 percent). Regenerated shoots were multiplied, rooted and hardened successfully. Kanamycin was found highly toxic to leaf explants and affected the regeneration rate of shoots even at lower concentrations. Infection time of 7 min was found the best for transformation of apple cultivar, which resulted 9.35 percent shoot regeneration frequency with 1.65 shoots per explant. 5 & 6 mg/l kanamycin along with 500 mg/l cefotaxime was used for the selection of the transformed shoots and resulted in 2-6 percent putative shoot regeneration. In PCR analysis of putative transformed shoots, 11 lines were found to be positive with the gene specific primers and nptII gene specific primers. Eleven transgenic lines were maintained in culture room which needs further molecular analysis.