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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20171
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Subject: Basic Sciences × Author: KUMARI, MANORMA × End date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    PRODUCTION AND CHARACTERIZATION OF LYTIC ENZYMES FROM Pseudomonas SPECIES ASSOCIATED WITH APPLE
    (UHF,NAUNI, 2017-12) KUMARI, MANORMA; KAUR, MOHINDER
    ABSTRACT The aim of present study was production and characterization of lytic enzymes i.e. protease and chitinase produced by fluorescent Pseudomonas sp. isolated from the normal and replant sites of apple orchards. Eleven Pseudomonas sp. were isolated from the replant sites of Rauni and Maggota of Shimla district. Four isolated strains of fluorescent Pseudomonas sp. from replant sites of apple were also used for screening of direct and indirect plant growth promoting activities like lytic enzymes (protease & chitinase), siderophores, phosphate solubilization and production of HCN, ammonia, plant growth regulators (auxins, cytokinins and gibberellins), antifungal and antibacterial activity. By 16S rRNA gene characterization, isolates Rn1 and Mgn1 showed 99.0% and 97.0% similarity with Pseudomonas brassicacearum KU139391 and Pseudomonas aeruginosa respectively. The pathogenic fungal isolates from replant sites of orchard at Rauni i.e. RF3 showed 99.0% similarity with Phytopythium litorale and from Maggota i.e. MF5 showed 99.0% similarity with Pythium ultimum by ITS gene sequencing. Optimization of media, time of incubation, temperature, pH, carbon, nitrogen and mineral sources were done for the production and characterization of protease & chitinase from four Pseudomonas strains L (Pseudomonas putida), M (Pseudomonas fluorescens), G and H (Pseudomonas aeruginosa), which were also used for mass multiplication of inoculum for field trials. Purification of protease and chitinase was done through Sephadex G-75 column chromatography. Through SDS-PAGE electrophoresis, the molecular weight of Protease was recorded as 31.0 to 43kDa and 26.0 to 38.0kDa for chitinase. This study also reported significant role of protease & chitinase in antagonistic activity against Dematophora necatrix, Phytopythium litorale and Pythium ultimum. Lytic enzymes (protease and chitinase) are responsible for the lysis and hyperparasitism of antagonists against deleterious fungal and bacterial pathogens. Lytic enzymes along with other plant growth promoting activities may be the reasons for better establishment and growth promotion of replanted apple. Treatments with bioformulations of consortia were found to be more effective in growth and establishment of replanted apple. Therefore these strains can be exploited for the management of replant problem of apple.