Thumbnail Image

Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

Browse

2019 - 201912020 - 20241
Reset filters
Subject: Basic Sciences × Author: DIMPLE × Start date: 1986 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    DEVELOPMENT OF MICROBIAL CONSORTIA FOR CONVERSION OF ORGANIC WASTE TO COMPOST AND TO EVALUATE ITS EFFICACY IN PLANT GROWTH PROMOTION IN Solanum lycopersicum L.
    (UHF Nauni, 2024-04-18) DIMPLE; Nivedita Sharma
    The present study evaluated seven potential hydrolytic enzyme-producing in-house bacterial isolates from the Microbiology laboratory of Basic Sciences. These potential bacterial isolates were morphologically and genotypically characterized and, identified as Bacillus subtilis (BM1), Bacillus amyloliquefaciens (SH8), Paenibacillus polymyxa (G7), Bacillus aerius (GC6), Brevibacillus parabrevis (C1), Bacillus axarequensis (P6), and Stenotrophomonas maltophilia (P9). These isolates produced various hydrolytic enzymes such as amylase, lipase, protease, cellulase, xylanase, pectinase, and laccase. These isolates showed maximum inhibition zones against various plant pathogens and also checked for its antibiogram activity. These were tested positive for various PGP attributes including ammonia, HCN, N2 fixation, protease, cellulase, chitinase, P-solubilization, siderophore, IAA, gibberellins, and cytokinin production in primary and secondary screening. Salt tolerance capability, inter-biocompatibility was also tested, and a liquid bioformulation was prepared for composting of different organic waste. Three solid based carriers i.e. charcoal, talcum, and cocopeat were tested for the formation of soild bioformulation. Charcoal was found to be the most effective solid carrier with the highest shelf life, outperforming talcum and cocopeat for the degradation of organic waste. These charcoal based compost was tested for its efficacy in Solanum lycopersicum L. under glass house conditions in a vegetable farm of UHF Nauni, Solan. The T5(biocompost) treatment showed the highest number of primary branches, fruits, average fruit weight, yield/plant, and plant height as compared to other treatments. A mature biocompost was produced under a pit experiment in Sultanpur village with a darker brown color, higher nitrogen, phosphorus, potassium, and Cu/Zn content as compared to the control. The compost was characterized using FTIR, SEM, and TEM, revealing its successful formation as amorphous, fibrous, and more granular than the control. This biocompost was then used in an open field conditions of Sultanpur where the highest yield of tomato and physiochemical properties of soil was observed. The T5 biocompost treatment demonstrated the highest number of primary branches/plant, fruits/plant, average fruit weight, yield/plant, fruit yield/plot, yield/ha, and plant height in field conditions. A solid bioformulation was developed using hydrolytic enzyme-producing isolates with PGP attributes to reduce chemical fertilizer usage in tomato cultivation which enhances crop production, and reduces pollution.
  • Thumbnail Image
    ThesisItemOpen Access
    SCREENING OF POTENTIAL LIGNOCELLULOLYTIC FUNGI ISOLATED FROM HIMALAYAN FORESTS AND TO ASSESS THEIR ROLE IN PINE NEEDLE DEGRADATION
    (UHF,NAUNI, 2019-11) DIMPLE; SHARMA, NIVEDITA
    ABSTRACT The present study was carried out to isolate lignocellulolytic fungi from the rotten wood of Himachal Pradesh (i.e. Solan, Shimla, Sirmour, Kangra and Chamba), their screening and optimization of laccase, cellulase and xylanase production. Among all the fungal isolates, R4, S5, SH2 and SH5 fungal strain were selected for enzyme production under submerged fermentation. The phenotypic characterization was done for their tentative identification i.e. Trichoderma sp. R4, Trichoderma sp. S5, Trichoderma sp. SH2 and Rhizopus sp. SH5. The molecular identification was done by using ITS 5.8S rRNA technique and S5 was identified as Trichoderma guizhouense |MN17050|. The extracellular hydrolytic enzyme production from these potential identified fungal strain was then subjected to solid state fermentation by optimizing the different environmental parameters i.e. temperature, substrate: moisture ratio, incubation time and pH using pine needles biomass as substrate under classical one factor approach. An enhanced production with laccase (6.45U/g), cellulase (37.20U/g) and xylanase (380 U/g) activity was obtained by Trichoderma sp. R4. Optimization process was then switched over to response surface methodology (RSM) and maximum enzyme production of Trichoderma sp. R4 in RSM with four responses i.e. laccase (6.90U/g), cellulase (37.86U/g), xylanase (398 U/g) and reducing sugar (47.98mg/g) was obtained. Highest production of enzymes activity was observed in pine needles biomass and Trichoderma sp. R4. had been used for further purification process. The culture filterate was subsequently partially purified by ammonium sulphate precipitation at 40% saturation level of laccase, 40% CMCase, 60% FPase, 50% β-glucosidase and 70% xylanase with purification fold of 3.06 (laccase), 2.20 (cellulase) and 1.59 (xylanase) with 82.94, 62.43 and 63.24 % recovery yield respectively. Gel exclusion chromatography was done for purification of hydrolytic enzymes with 5.36, 5.51 and 6.33 purification fold and 45.77, 61.33 and 60.23 % recovery yield for laccase, cellulase and xylanase respectively. The molecular mass of purified laccase (40.0kDa), cellulase – CMCase (45.0kDa), FPase (31.0kDa), β-glucosidase (29.0kDa) and xylanase (65.0kDa) was obtained by using SDS-PAGE. The maximum enzymatic degradation of pine needles was obtained in purified fractions of enzymes of Trichoderma sp. R4 with a release of 76.75 mg/g reducing sugars. The present study strongly proves the success of optimization of different parameters for enhancement in level of enzyme i.e. laccase, cellulase, xylanase and degradation of pine needles with a cost-effective approach of enzyme production using a cheap and abundant pine needle waste as a carbon source.