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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    STUDY ON NUTRITIONAL AND ANTIOXIDANTS PROFILE OF SOME SPECIALTY RICE CULTIVARS OF ODISHA
    (2021) Bal, Rajesh; Kandali, Ranjan
    Odisha has been considered as the center of genetic diversity for cultivated rice and is endowed with numerous cultivars of specialty rice which have great market potentiality. These traditionally cultivated cultivars were rich in nutritional and healthpromoting components. However, these cultivars were on the verge of disappearing because farmers were shifting to high-yielding types, which had great market potential and provide them with financial security. There is dearth of information on the nutritional aspect of these traditional unexplored rice cultivars. The present investigation entitled 'Study on the nutritional and antioxidant profiles of several specialty rice cultivars from Odisha' was carried out in the laboratory of Department of Biochemistry & Agricultural Chemistry, Assam Agricultural University, Jorhat. The objective of this study was to assess the nutritional value and antioxidant activity of these specialty rice cultivars from Odisha. A total of eleven cultivars was used in the study, out of which eight were aromatic (Rajendra Bhagabati, Nua-Dhusara, Kalajeera, Nua-Kalajeera, Nua-Chinikamini, Lall Basana, Nua- Purnabhog and, Nilabati) two were pigmented rice (black rice Kalabati and red rice Jaghabalia), and one as a check variety (Kalachampa). The moisture content, reducing sugar, total soluble sugar, starch, total carbohydrate, crude protein, soluble protein, crude fat, crude fibre, ash, total phenolics, total anthocyanin, and total antioxidant activity of the cultivars were determined. The cultivars were collected from the farmer‟s field at different places of Odisha as well as from National Rice Research Institute, Cuttack, Odisha. The results showed that the moisture content of all the cultivars were lower than the check variety Kalachampa which recorded 11.914± 0.08 % on a fresh weight basis. The highest per cent starch content was recorded in Nua-Kalajeera (82.791± 0.96) followed by Kalajeera (80.605± 1.173) and Kalabati (79.896± 0.176) while lowest in Nua-Purnabhog (59.612± 0.328). Cultivars varied significantly with respect to starch content (CD0.05=3.407). The highest starch content was observed in aromatic rice cultivar Nua-Kalajeera (82.7g% DW) followed by Kalajeera (80.6 g% DW) and Kalabati (79.89 g% DW) and the least recorded in Nua-Purnabhog (59.6 g% DW). Per cent crude protein content varied significantly (CD0.05=0.494) and was recorded highest 7 in Nua-Dhusara (10.384± 0.294) followed by Nua-Purnabhog (9.384± 0.147) and Kalabati (8.552± 0.147). Per cent crude fibre content was found highest in Kalabati (0.984± 0.073). Cultivars differ significantly (CD0.05=0.239) with respect to their ash content with the highest recorded in Nua-Kalajeera (2.423± 0.041). The highest total phenol content (g GAE/100gm) was recorded in Jaghabalia (0.833± 0.014) followed by Kalabati (0.701± 0.001). On the other hand, the variation seen in total anthocyanin content among the cultivars was significant. The highest anthocyanin content was in black pigmented rice cultivar Kalabati (5.016 mg Cya-3-gluE /100g FW) followed by aromatic rice Nua Kalajeera (1.204 mg Cya-3-gluE /100gm) and Kalajeera (1.132 mg Cya-3-gluE /100gm FW). The least was recorded in Lall Basana (0.310 mg Cya-3- gluE/100gm FW). The antioxidant activity measured as per cent DPPH free radical scavenging activity was highest in Jaghabalia (55.324± 0.352) followed by Kalabati (48.576± 2.876). The high antioxidant activity of the cultivar Kalabati might be due to high anthocyanin and total phenol content. The selected pigmented and scented specialty rice cultivars were found to be superior to high-yielding variety Kalachampa in different nutritional parameters in the grain such as carbohydrates, Crude protein, crude fiber, Crude fat, total phenolic content, total anthocyanin, and antioxidant activity. Nua-Purnabhog, with a low starch content of 59.61 g percent DW, might be a lowstarch dietary option. It will make it easier for persons with hyperglycemia or diabetes to eat low-starch cereals. From the results of the present investigation, it could be concluded that the black rice cultivar Kalabati appeared to be a promising cultivar with a superior nutritional composition and antioxidant activity and thus has a great market potentiality.
  • ThesisItemOpen Access
    STUDY ON NUTRITIONAL AND ANTIOXIDANTS PROFILE OF SOME SPECIALTY RICE CULTIVARS OF ODISHA
    (2021) Bal, Rajesh; Kandali, Ranjan
    Odisha has been considered as the center of genetic diversity for cultivated rice and is endowed with numerous cultivars of specialty rice which have great market potentiality. These traditionally cultivated cultivars were rich in nutritional and healthpromoting components. However, these cultivars were on the verge of disappearing because farmers were shifting to high-yielding types, which had great market potential and provide them with financial security. There is dearth of information on the nutritional aspect of these traditional unexplored rice cultivars. The present investigation entitled 'Study on the nutritional and antioxidant profiles of several specialty rice cultivars from Odisha' was carried out in the laboratory of Department of Biochemistry & Agricultural Chemistry, Assam Agricultural University, Jorhat. The objective of this study was to assess the nutritional value and antioxidant activity of these specialty rice cultivars from Odisha. A total of eleven cultivars was used in the study, out of which eight were aromatic (Rajendra Bhagabati, Nua-Dhusara, Kalajeera, Nua-Kalajeera, Nua-Chinikamini, Lall Basana, Nua- Purnabhog and, Nilabati) two were pigmented rice (black rice Kalabati and red rice Jaghabalia), and one as a check variety (Kalachampa). The moisture content, reducing sugar, total soluble sugar, starch, total carbohydrate, crude protein, soluble protein, crude fat, crude fibre, ash, total phenolics, total anthocyanin, and total antioxidant activity of the cultivars were determined. The cultivars were collected from the farmer‟s field at different places of Odisha as well as from National Rice Research Institute, Cuttack, Odisha. The results showed that the moisture content of all the cultivars were lower than the check variety Kalachampa which recorded 11.914± 0.08 % on a fresh weight basis. The highest per cent starch content was recorded in Nua-Kalajeera (82.791± 0.96) followed by Kalajeera (80.605± 1.173) and Kalabati (79.896± 0.176) while lowest in Nua-Purnabhog (59.612± 0.328). Cultivars varied significantly with respect to starch content (CD0.05=3.407). The highest starch content was observed in aromatic rice cultivar Nua-Kalajeera (82.7g% DW) followed by Kalajeera (80.6 g% DW) and Kalabati (79.89 g% DW) and the least recorded in Nua-Purnabhog (59.6 g% DW). Per cent crude protein content varied significantly (CD0.05=0.494) and was recorded highest 7 in Nua-Dhusara (10.384± 0.294) followed by Nua-Purnabhog (9.384± 0.147) and Kalabati (8.552± 0.147). Per cent crude fibre content was found highest in Kalabati (0.984± 0.073). Cultivars differ significantly (CD0.05=0.239) with respect to their ash content with the highest recorded in Nua-Kalajeera (2.423± 0.041). The highest total phenol content (g GAE/100gm) was recorded in Jaghabalia (0.833± 0.014) followed by Kalabati (0.701± 0.001). On the other hand, the variation seen in total anthocyanin content among the cultivars was significant. The highest anthocyanin content was in black pigmented rice cultivar Kalabati (5.016 mg Cya-3-gluE /100g FW) followed by aromatic rice Nua Kalajeera (1.204 mg Cya-3-gluE /100gm) and Kalajeera (1.132 mg Cya-3-gluE /100gm FW). The least was recorded in Lall Basana (0.310 mg Cya-3- gluE/100gm FW). The antioxidant activity measured as per cent DPPH free radical scavenging activity was highest in Jaghabalia (55.324± 0.352) followed by Kalabati (48.576± 2.876). The high antioxidant activity of the cultivar Kalabati might be due to high anthocyanin and total phenol content. The selected pigmented and scented specialty rice cultivars were found to be superior to high-yielding variety Kalachampa in different nutritional parameters in the grain such as carbohydrates, Crude protein, crude fiber, Crude fat, total phenolic content, total anthocyanin, and antioxidant activity. Nua-Purnabhog, with a low starch content of 59.61 g percent DW, might be a lowstarch dietary option. It will make it easier for persons with hyperglycemia or diabetes to eat low-starch cereals. From the results of the present investigation, it could be concluded that the black rice cultivar Kalabati appeared to be a promising cultivar with a superior nutritional composition and antioxidant activity and thus has a great market potentiality.
  • ThesisItemOpen Access
    STUDIES ON PROFILE OF PHENOLIC COMPOUNDS IN A FEW PIGMENTED RICE VARIETIES OF ASSAM
    (2021) Sahewalla, Surbhi; Das, Priyanka
    Considering the growing economic importance together with health benefits of pigmented rice, the present research work ―Studies on profile of phenolic compounds in a few pigmented rice varieties of Assam‖ was carried out. In the present study, fourteen rice varieties were considered which included eleven traditional varieties and two recent varieties developed at AAU. The results were compared with the well known traditional pigmented rice variety from Manipur, Poreiton Chakhao. There was significant difference among the varieties regarding content of different phenolic compounds. The total phenol content (TPC) ranged from 493.611 mg GAE/100g (TTB Black Rice, AAU 1347-2, line 11) to 37.101 mg GAE/100g (Amona Bao). The total flavonoid content (TFC) ranged from 151.667 mg QE/100g (TTB Black Rice, AAU 1347-2, line 11) to 53.316 mg QE/ 100g (Amona Bao). The total anthocyanin content (TAC) ranged from 13.904 mg cyanidin-3- chloride equivalent (TTB Black Rice, AAU 1347-2, line 11) to 1.006 mg cyanidin-3- chloride equivalent (Dol Bao). The amount of rice showing 50% DPPH free radical scavenging activity (IC 50 value) ranged from 6.610 mg (TTB Black Rice, AAU 1347-2, line 11) to 29.376 mg (Amona Bao). The traditional rice varieties of Assam, specially Betu, showed antioxidant activity equivalent to Chakhao-1. The HPLC analysis revealed presence of both cyanidin-3-glucoside and peonidin-3-glucoside in the black pigmented rice varieties viz. TTB Black Rice (AAU 1491-4, line 7), Chakhao-1, Chakhao-2 and TTB Black Rice (AAU 1347-2, line 11). However in the red rice varieties analysed (Balam and Nepali Chakuwa), these two anthocyanins were not detected. The present study revealed both the varieties developed at TTB Black Rice (AAU 1491-4, line 7) and TTB Black Rice (AAU 1347-2, line 11) showed promising result regarding content of phenolic compounds including anthocyanin and antioxidant activity.
  • ThesisItemOpen Access
    STARCH DIGESTIBILITY AND ESTIMATED GLYCEMIC INDEX OF RICE DIFFERING IN AMYLOSE CONTENTS
    (2021) Aranha, Deena Lyrisha; Baishya, Samindra
    Rice (Oryza sativa L.) is the most important cereal crop worldwide and serves as the major energy source in human nutrition. Starch digestion rate is an important consideration for rice consumers, particularly for diabetics. In the present study, eleven rice varieties differing in amylose contents were assessed for their variation in starch digestibility. An in vitro enzymatic starch digestion method was applied to estimate the glycemic index of rice varieties based on the kinetics of starch hydrolysis. Moisture content in the studied varieties ranged from 11.2% to 13.56%, reducing sugars from 0.33 to 0.65g/100g and starch content from 62.33 to 88.17%, with amylose content from 1.6% to 24.34% and amylopectin content from 75.65 to 98.39% on a dry weight basis. In vitro starch digestion resulted in readily available glucose (RAG) content from 44.96 to 95.4% and free glucose (FG) from 0.102 to 0.211%. The digestible and available starch fractions were calculated. Rapidly digestible starch (RDS) ranged from 40.33 to 85.72% and slowly digestible starch (SDS) from 2.59 to 41.99%. The resistant starch (RS) varied between 6.09% and 20.01%. The kinetics of in vitro starch digestion was determined and used for the calculation of hydrolysis index (HI) with which the glycemic index (eGI) was finally estimated. Starch digestibility and eGI varied widely among the rice varieties. The hydrolysis index ranged from 35.88 to 90.25 and with the lowest HI value of 35.88, Gitesh had the lowest eGI of 59.41, followed by Bahadur and Ranjit with eGI of 62.79 and 63.27 respectively. All these three varieties along with Prafulla, Numali, and TTB Black rice were found to be medium glycemic index rice. Sam Chakuwa, Lahi Chakuwa, Betguti, Joha Bora, and Ronga Bora were the high glycemic index rice.
  • ThesisItemOpen Access
    ANALYSIS OF RICE IN ARSENIC CONTAMINATED AREAS OF JORHAT DISTRICT
    (AAU, Jorhat, 2021) Dutta, Amrit; Rathi, Sunayana
    Rice (Oryza sativa L.) provides food for more than three billion people. Approximately 90% of rice production and consumption is reported in Asia. However, rice consumption may pose a greater threat because of the arsenic (As) accumulation in rice grains and thus serves a vital source of arsenic (As) exposure in humans. Arsenic (As) is the 20th abundant component on the Earth’s crust and considered one of the most toxic metalloids. Based on the Agency for Toxic Substances and Disease Registry list 2017, Arsenic (As) is amongst the most hazardous materials that could be poisonous to humans. In the present investigation, total arsenic accumulation in different part of rice plant (var. Ranjit) of two different locations (Nagajanka and Titabar) and different biochemical parameters associated with accumulation in rice plant was analysed. It was found that the arsenic (As) accumulation in roots ranged from 0.851 to 1.655 ppm, straw from 0.495 to 0.645 ppm, leaf from 0.426 to 0.640 ppm, husk from 0.162 to 0.213 ppm and grains from 0.094 to 0.147 ppm. With decreasing arsenic accumulation, the catalase activity increases significantly and ranges in the roots from 53.470 to 60.586 units/min/g fw and in the grains from 86.253 to 92.186 units/min/g fw. A significant decrease in the activity of ascorbic acid oxidase (AOX) was observed which ranges in roots from 4.482 to 5.333 μmoles ascorbic acid disappeared /min/g fw and in grains ranges from 1.493 to 1.753 μmoles ascorbic acid disappeared /min/g fw. It was also observed that hydrogen peroxide (H2O2) content in roots ranges from 0.122 to 0.151 μM/g fw and in grains ranges from 0.055 to 0.069 µμM/g fw; proline content in roots ranges from 82.309 to 95.592 μg/g fw and in grains ranges from 7.666 to 9.172 μg/g fw; malondialdehyde (MDA) content in roots ranges from 3.066 to 3.311 μM/g fw and in grains ranges from 0.127 to 0.255 μM/g fw decreases significantly with decrease in the arsenic accumulation in different parts of rice plant. In the present study it was concluded that the arsenic accumulation the rice grain (0.094 to 0.147 ppm) is below the permissible limit whereas in straw (0.495 to 0.645 ppm) it is above the permissible limit of 0.20-0.25 ppm (FAO, 2016). However, with decreasing arsenic (As) accumulation it was observed that catalase activity increases whereas ascorbic acid oxidase activity (AOX), hydrogen peroxide (H2O2) content; proline content and malondialdehyde (MDA) content decreases significantly.
  • ThesisItemOpen Access
    ALTERNATIVE USE OF WASTE OF TEA GARDENS AS POTENTIAL SOURCE OF BIOMOLECULES AND BIOCHAR
    (AAU, Jorhat, 2021) Goswami, Naina; Das, Priyanka
    In the present study, factory tea waste and garden tea waste (pruned leaf) were collected from Cinnamara Tea Estate, Jorhat and tea garden of Assam Agricultural University, Jorhat for production of biochar and to extract crude TRIA and to know their properties and effects on plant growth, respectively. Garden tea waste (pruned leaf) was used for extraction ofcrude n-triacontanol (TRIA) using conventional Soxhlet extraction method. Yield of crude TRIA was 4.45%, on dry weight basis. The potential of crude n -triacontanol as a plant growth regulator for brinjal plant was explored and compared with the commercial TRIA (Miraculan). In terms of plant height, flower count, leaf count, chlorophyll content and mineral content (N%, P%, K%) of leaves, crude ntriacontanol extract (petroleum ether extract) showed better results than the control (distilled water). Factory tea waste was used for the production of biochar using pyrolysis. Biochar was produced by two method using charring device (Method 1) and muffle furnace (Method 2). The yield of biochar was from 71.07% to 77.21%. There were significant differences among the biochar prepared by the two methods regarding the physical and chemical properties. It was observed that the percent moisture content, bulk density, apparent water holding capacity of biochar prepared from charring device (Method 1) was 6.1%, 0.19 g/cm3, 80.17%, respectively and from the muffle furnace (Method 2) was 3.34%, 0.21g/cm3, 84.67%, respectively. Additionally, chemical properties like percent total carbon content, nitrogen content, phosphorus content, potassium content, cation exchange capacity, crude fibre and pH for method 1 was 50.9%, 3.94%, 0.0377%, 0.071%, 11.87 cmole/kg, 1.63% and 7.88 respectively and for the method 2 was 60.24%, 4.54%, 0.0441%, 0.073%, 15.77 cmole/kg, 1.13% and 7.9, respectively. The present study revealed that the waste leaves from the pruned branches possess plant growth promoting properties, which may be commercially exploited in the form of crude extract. Production of biochar using factory tea waste may also be a good option for carbon sequestration and also for soil application for crop growth.
  • ThesisItemOpen Access
    BIOCHEMICAL STUDY ON TRADITIONAL RICE PRODUCTS OF ASSAM WITH SPECIAL REFERENCE TO RESISTANT STARCH
    (AAU, Jorhat, 2017-07) Banik, Rajesh; Das, Priyanka
    Rice is the most important food crop of the world after wheat. South East Asian countries are heavily reliant upon rice for their dietary energy supply. 70% of total arable agricultural land of India lies under the rice cultivation. In North Eastern region, rice is the main staple food and in Assam there are several traditional rice based processed products which are also largely used as food. These are bhoja chaul, sandah guri, korai, popped rice, flaked rice, puffed rice, komal chaul (soft rice) and hurum. The moisture content, on wet basis varied from 5.83-11.61%. on dry weight basis, the crude protein content of different rice products ranged from 7.74-9.40%, the lowest in puffed rice and the highest in popped rice. The total ash content was determined on dry weight basis and it ranged in between 0.61-4.33%. The crude fiber percentage was the highest (1.04%) in bhoja chaul and sandah guri and the lowest (0.63%) in popped rice. The crude fat content is the lowest in hurum (0.11%) and the highest in korai (0.31%). The total carbohydrate percentage of these traditional rice products ranged between 49.33-67.55% and the highest in sandah and the lowest in flaked rice. The total starch content (on dry weight basis) was found to be the highest (58.08%) in popped rice and the lowest (39.37%) in korai, whereas, the resistant starch content was found to be highest (5.42%) in sandah and the lowest (3.24%) in puffed rice. The present study indicated that the rice based traditional processed products of Assam are good sources of carbohydrate, proteins and crude fiber. The said products are having good amount of resistant starch, which is very much useful for the better health. In future, further studies are required for the determination of other nutrients such as different micronutrients, and physical properties of the rice based traditional products of Assam, that may reveal useful information for the human health.
  • ThesisItemOpen Access
    Phytochemical Characterization of Some Ginger Cultivars from Tripura
    (AAU, Jorhat, 2017-07) Nandy, Pratul Kumar; Baruah, A.M.
    Ginger (Zingiber officinale Rosc.), under the family Zingiberaceae, is one of the most famous spices all over the world. It is commonly known as Ada (Assamese and Bengali name), originated from South East Asia. Ginger plants are used for thousands of years in Indian health care systems for their biological activities. Ginger is also used as home remedy and is of immense value in treating various gastric ailments like constipation, belching, bloating, gastritis, epigastric discomfort, gastric ulcerations, indigestion, and vomiting. Ginger with spicy, penetrating, pungent, and slightly biting flavor finds extensive use in foods. The present study was carried out to investigate the some major secondary phytochemicals of four different ginger cultivars from Tripura. The moisture content was highest in Gandacherra cultivar (86.167g/100g) and lowest in Satpara cultivar (82.580g/100g). Total phenol content was highest in Belonia cultivar (0.733g/100g) and lowest in Gandacherra cultivar (0.395g/100g).The total flavonoid content was highest in Satpara cultivar (0.379g/100g) and lowest in Gandacherra cultivar (0.103g/100g). The total tannin content was highest in Belonia cultivar (4.237g/100g) and lowest in Satpara cultivar (2.793g/100g).The total soluble sugar content was highest in Gandacherra cultivar (2.760g/100g) and lowest in Dharmanagar cultivar (1.243g/100g). The total crude fat content was highest in Satpara cultivar (8.267g/100g) and lowest in Dharmanagar cultivar (6.967g/100g). The total crude fiber content was highest in Satpara cultivar (6.040g/100g) and lowest in Gandacherra cultivar (4.685g/100g). The antioxidant activity (by DPPH, 2, 2-diphenyl-picrylhydrazide) reveals that the IC50 was highest in Gandacherra cultivar (0.853μg/ml) and lowest in Belonia cultivar (0.809 μg/ml). The total oil content was highest in Dharmanagar cultivar (4.180ml/100g) and lowest in Gandacherra cultivar (3.275ml/100g). The highest acid value was recorded in Belonia cultivar (19.635μg KOH/mg fat) and the lowest acid value was recorded in Gandacherra cultivar (11.220μg KOH/mg fat). The highest iodine value was observed in Belonia cultivar (71.063g I2/100g fat) and the lowest iodine value was observed in Dharmanagar cultivar (64.719g I2/100g fat). The highest saponification value is observed in the Gandacherra and Satpara cultivar (196.350 mg KOH/g fat) and the lowest saponification value was observed in Belonia cultivar (168.300 mg KOH/g fat).
  • ThesisItemOpen Access
    Studies on chemical composition and medicinal property of Amomum aromaticum Roxb.- a rare species of cardamom found in North East India
    (AAU, Jorhat, 2017-07) Das, Poulami; Kandali, R.
    Wild cardamom (Ammomum aromaticum Roxb.) has been recently discovered in large patches of forests of Tripura by forest department during 2014-15. It is popularly known by the local tribals as „Beering‟ in their vernacular language, whose stump is used in the local culinaries to induce aroma to the dishes. Botanically it belongs to Zingiberaceae family. Processed dry fruit is the economic produce which can be used largely as spice because of its sweet aroma and in the ayurvedic medicine because of its medicinal value. As per the available literature it is mentioned to be found in the eastern Himalayan track and Chittagong hill track. It is typically found in patches along the banks of streams and streamlets. It is a notified forest product and can be collected by the forest dwellers and after being processed (drying) on desi bhatta can be sold out to the authorized traders who are dealing with aromatic oils and medicinal products. The present investigation was intended to study the chemical composition and medicinal property of A. aromaticum Roxb.- a rare species of cardamom found in North East India. The plant materials were collected from forest of Tripura- Kunjaban village, Kalyanpur block, Khowai district and authenticated. The morphological data were taken from the mature plant to narrate the botanical information. Leaf and seed samples of this species were analysed for total alkaloids and total phenolics. The essential oil was extracted from seed and the volatile components were identified. The antimicrobial and antioxidant activity of the plant extracts were determined. From the results of the present investigation it was observed that a significant variation in the total phenolic content in the leaf and seed was obtained which were 12.7 mg/g and 10.1 mg/g, respectively. The alkaloid content of the leaf was found to be 1.27 g/100g and in case of seed it was 4.2 g/100g on dry weight basis. The essential oil was extracted from both dried & raw capsules of the matured plant by hydro-distillation method. The fresh moisture content in capsules at harvest was 75 per cent and the moisture content reduced to 14 per cent in case of seeds which were sundried for 10 days. The essential oil content in seeds of A. aromaticum was found to be 2.0ml/100g in raw fresh capsules and 1.0 ml/100g in sun dried capsules. GC-MS analysis of essential oil revealed the presence of components such as. Myrcenol, D-limonene, P-mentha-1- en-9-ol, Linalool, Isopulegol, P-menth-8-en-1-ol, Linalyl acetate, Sabinene hydrate, α- terpineol, Eucalyptol, Terpinyl acetate, Menthol, Nanocosane and 2,3- pinanediol. In addition, a few new components have also been detected such as 4,6 di-tertbutylresorcinol, 5-iodo-2,7-dioxa-tricyclo{4,3,1,0(3,8)}decane, triacontane, 1 monolinoleoyl glycerol, trimethyl silyl ether, eicosanoic acid, di-N-decylsulfone and Pentatriacontane. The antimicrobial activity of different solvent extracts of leaf and seeds of A. aromaticum Roxb was evaluated against. Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The essential oil extracted from the seeds did not show any antimicrobial activity against E. coli, S. aureus and B. subtilis. The hydrodistilled volatile oil from seeds and ethanol extracts of the seeds and leaves did not show antimicrobial activity. On the other hand, the methanol extract of seeds showed potential antimicrobial activity against these human pathogens. The antibiotic streptomycin was used as positive control and 80 % methanol was used as negative control in this experiment. The zone of inhibition for E. coli, S. aureus and B. subtilis. was found to be 15.2 mm, 17.4 mm and 14.5 mm, respectively. The antioxidant activity was determined in the methanol extract obtained from both leaf and seed of this species. Both the extracts showed antioxidant activity. The percent inhibition of DPPH was observed to be 74.1 for seed extract and 60.8 for leaf extract. The IC50 value for leaf and seed extract were 0.815μg/ml, 0.641μg/ml respectively. Moreover, the genomic DNA was extracted from the mature fresh leaf tissues of A. aromaticum Roxb and the extraction procedure was standardized. The quantity of the extracted DNA as determined by “Nanodrop-1000” (make: thermo-scientific) was 1282.09 ng/μl. The purity of the extracted DNA was determined from the ratio of optical density at 260 and 280 nm respectively which was found to be 1.79 (A260:280). Vast medicinal plant resources of North East India have not been fully identified, inventoried and characterized. It is of utmost importance that these should be characterized and evaluated in the light of modern scientific approaches, which may lead to the development of some new drug molecules that can combat various side effects of the commercially available synthetic drugs, and thereby reducing the cost of medication. So a detail study about this traditionally underutilized herbal spice species- Amomum aromaticum Roxb. will help to develop new drugs and a number of herbal tonic or feed additives. More studies will be required to find out the favourable conditions to achieve the full potential of the plant in order to establish this plant as one of the important spice species.