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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme.
The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered.
The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers.
In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University
A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.
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ThesisItem Open Access EFFECT OF DESICCATION ON CALLUS CULTURES IN COTTON(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2009-06-12) ZAMBRE, SUPRIYA MANOHAR; VAIDYA, E.R.ThesisItem Open Access MOLECULAR AND BIOCHEMICAL CHARACTERIZATION FOR WATER STRESS TOLERANCE IN WHEAT GENOTYPES(Dr.Panjabrao Deshmukh Krishi Vidyapeeth, Akola., 2017-01-18) TANDALE, RAM MHATARDEO; Gahukar, Dr. S. J.Drought affects crop growth and yield by altering metabolic and physiological processes. Plants tolerate drought through osmotic adjustment, stomatal resistance, increased water uptake and accumulation of waxy layer. Cultivars possessing drought tolerant traits may be promising candidates for drought prone regions. The present study was planned for molecular and biochemical characterization for water stress tolerance in wheat genotypes. In this study various morphological, physiological, biochemical and molecular observations were recorded. Drought stress was created with different concentrations of PEG-6000 (-2, -4, -6, and -8 bars) and by withholding of water at moderate and sever level. On the morphological basis, all cultivars exhibited decline in germination percentage, speed of germination, shoot-root length, coleoptile length, seedling fresh and dry weight under water stress environments. While on biochemical basis proline has direct relationship with osmotic stress and water stress while total chlorophyll content has inverse association with osmotic stress and water stress. On the physiological basis, with increase in water stress photosynthesis rate, relative water content decrease while epicuticular wax content increased with an increase in osmotic stress and water stress conditions. All these parameters finally extremely affect the yield of wheat genotype. At the end for drought response, wheat cultivars were evaluated on molecular basis. A set of trait specific 18 SSR primers were used for identification of drought tolerant genotypes. From which 16 primers gave polymorphic result and amplified unique band as result of drought tolerance. AKW-381 followed by AKAW-4627, AKAW-4210-6, AKAW-4498 and AKAW-4842 performed best at various stress levels for germination percentage, speed of germination, root-shoot fresh and dry weight, total chlorophyll content, proline, Rate of photosynthesis, wax content, and relative water content. The performance of HD-2189, GW-322, NIAW-917 and AKAW-3722 was poor. On molecular basis expected size unique band was amplified in the tolerant genotypes AKW-381, AKAW-4627, AKAW-4210-6, AKAW-4498 and AKAW-4842. It can be concluded that water stress levels had substantial effect on germination and seedling growth. Morphological, biochemical, physiological and molecular analysis revealed that adequate genetic difference for drought tolerance existed in the tested genotypes. AKW-381 and AKAW-4627 may prove a promising parent material for breeding drought tolerant wheat cultivars. Further molecular investigations are suggested to assess the genetic basis of drought tolerance. AKAW-4627, AKAW-4210-6, AKAW-4498 and AKW-381 may be considered better genotypes for low rainfall or drought prone areas.ThesisItem Open Access MOLECULAR AND GENETIC ANALYSIS OF PUTATIVE TRANSFORMANTS IN TRANSGENIC PIGEONPEA.(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-12-05) KADAM, KARTIKI DADASAHEB; Akhare, Dr. A. A.The gram pod borer (Helicoverpa armigera Hubner) is the most serious insect pest of pigeonpea. It is highly susceptible to the insecticidal proteins of Bacillus thuringiensis (Bt). A codon-optimized chimeric Cry1Aabcgene of Bt driven by a constitutive promoter was introduced pigeonpea (PKV Tara). Molecular analyses were carried out by most of worker in T1 and T2 generation. From 7 plants of T3 generation total 57 progenies were tested for PCR from which 47 plants were seen to be positive through PCR analysis. 38 plants of T1 generation from DK+ material were tested for PCR analysis through gene specific primers from which 22 plants were seen to be positive. Kamble in year 2014-15 attempted 130 embryo infections with Agrobacterium strain EHA105 harboring Cry1Aabc and NptII gene out of which 73 were survived but only two plants were confirmed positive through PCR. T1 and T2 generation of these two plants namely A and B were sown. Three plants from eachwere selected for PCR confirmation. From two positive plants total 6 progenies from T1 generation and 3 plants from T2 generation were tested for PCR analysis all the 6 plants from T1 generation were seen to be positive while from T2 generation out 3 only two plants were positive. Sample population size of T1 and T2 plants was too less to study inheritance pattern. In order to study Mendalian inheritance pattern in T3 progeny PCR analysis for the amplification of the cry1Aabc gene and NptII gene was carried out. The plants from same parents plant was consider together for the inheritance study. For undertaking the Chi-square test for segregation the minimum population size of 6 was taken. Calculated x2 value is less than table value in all the plant progenies. Thus plants for the amplification of the Cry1Aabc gene and NptII gene and progeny of each clone shows 3:1 (presence: absence) segregation pattern. For detached leaf bioassay 19 positive plants and one negative plant which were confirmed by DNA extraction and PCR amplification with NptII and Cry1Aabc gene specific primers were tested using leaf feeding assay with neonate larvae of Helicoverpa armigera. 20 putative transformants of pigeonpea were subjected to insect (Helicoverpa armigera) bioassay using neonates of Helicoverpa armigera and young trifoliate leaves and WT (non-transformed) PKV Tara was used as control. Thus the result revealed that the level of Cry protein expressed in positive plants were able to reduce the leaf damage. Thus, it was concluded from above results that like positive plants selected for bioassay study having potential for providing tolerance against Helicoverpa armigera. The further confirmation in subsequent generation is required to come to more robust conclusion.ThesisItem Open Access INTROGRESSION AND CHARACTERIZATION OF BACKCROSS POPULATION OF SORGHUM.(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2018-02-06) RAUT, DIMPAL SHESHRAO.; Gahukar, Dr. S. J.The present investigation entitled “Introgression and characterization of backcross population of sorghum” was carried out at Molecular Breeding Laboratory, Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola in academic year 2015-2016. The study was aimed at introgression the glossiness and trichome density resistant QTLs using specific SSR markers. A total 80 BC4 plant progenies were obtained by crossing AKSV-13R X IS18551, all these progenies were evaluated for phenotyping and genotyping using specific foreground and background primers. Wide morphological variation was observed among the genotypes for the shoot fly resistance component traits viz., pigmentation, leaf glossiness, seedling with eggs, dead hearts, trichome density and yield. Phenotypic correlation between the component traits of shoot fly resistance were estimated based on individual progeny from each of BC4 population under study. Yield showed highly significant positive association with trichome density in BC4 population under study. These traits could be utilized as simple criteria for phenotypic selection for resistance to shoot fly in sorghum. Foreground selection of BC4 progenies using markers associated with genomic region for glossiness and trichrome density revealed specific donor parent type alleles in plant no. BC4-2, BC4-10, BC4-14, BC4-17, BC4-19, BC4-25, BC4-54, BC4-60, BC4-76, BC4-79 from cross AKSV-13 X IS18551. Background selection of BC4 revealed that recurrent parent type of in plant no. BC4-1, BC4-6, BC4-13, BC4-17, BC4-35, BC4-41, BC4-43, BC4-55, BC4-68, BC4-73 population of genome recovery of recurrent parent. Amplification with foreground markers revealed specific allele recovery from donor parent (IS18551) in BC4 population. Population plant no. BC4-3, BC4-50 in foreground markers and plant no. BC4-5, BC4-70 in foreground markers showed maximum alleles with expected size.ThesisItem Open Access IN-PLANTA TRANSFORMATION OF SOYBEAN (Glycine max L.) TO ENHANCE SALINITY AND DROUGHT TOLERANCE(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-10-10) LUNGE, AKSHAY RAJENDRA; Jadhav, Dr. P. V.In the present investigation entitled “In-Planta transformation of soybean (Glycine max L.) to enhance salinity and drought tolerance” was under taken with the view to develop In-planta regeneration system for elite soybean genotype. The study was carried out at Biotechnology Centre, Dr. PDKV, Akola during year 2015-16. In vitro culture and genetic transformation of soybean is difficult due to its recalcitrant nature. Establishment of gene transfer procedure is prerequisite to devolved transgenic plant of soybean in a shorter period. Therefore, the transformation was perform using the most popular soybean cv., JS-335 to optimize the factor influencing transformation efficiency through Agrobacterium tumefaciens based in-planta transformation using LBA4404 strain harboring gene, PDH45. In the present study four strategies were employed for in-planta transformation i.e. infection to seed, infection to seed and then incubation for 24 h in Agrobacterium, infection to every developing stage and infection to flowering and pod filling stage. Several parameters, such as optical density, incubation time, acetosyringone concentration influencing In-planta transformation, have been evaluated in this study. In seed radicle treatment, number of flower and pod formation was highest among the treatments. All the plant samples (T1) tested were found to be negative after PCR confirmation with gene specific primer. Similarly, in treatment injected to seed radicle and incubated for 30 min. out of 50 sample two samples were found to be positive after PCR analysis with gene specific primer. Putative transgenics were screened by PCR confirmation. It helped to eliminate non-transgenic. The method also decreased the time and effort involved in the screening process The procured plasmid containing PDH 45 gene was transformed into the Agrobacterium using In-planta method without using helper strain and confirmed by restriction digestion and the gene specific set of primers.ThesisItem Open Access OPTIMIZATION OF MICROPROPAGATION PROTOCOL OF SANDAL (Santalum album L.).(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-10-10) WASHIMKAR, HARIDAS BALIRAM; Gahukar, Dr. S. J.Present investigation aimed, to produce disease and virus free plantlets, to standardize explant type and media combinations amenable for tissue culture, to in vitro micro propagation of Santalum album was conducted in Completely Randomized Design at Centre of excellence in Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist. Akola (M.S.) during the academic year 2015-2017. Survival percentage of nodal explants after two and three week’s treatment involving MS + BAP 1mg/l + kinetin 1mg/l recorded highest survival percentage of 70.00% and 68.33percent respectively. For initiation of shoot bud after two weeks the nodal explants were inoculated on MS + BAP + Kin @ 1mg/l The highest percent of survival was recorded i.e.63.33 percent. After three weeks of inoculation on MS + BAP + Kin @ 1mg/l each the highest percent of shoot bud induction was i.e. 61.66 percent. For multiple shoot induction after three and six weeks treatment involving MS + BAP 3 mg/l + kin 1 mg/l + ADS 2mg/L highest survival of multiple shoot were recorded, in three weeks (5.06) and after six weeks (2.81). For Seed germination when MS 3 % without GA3 and MS 3%, 4%, and 6% with GA3 1mg/l was tried the highest seed germination was observed (86.66) when MS 3%+ GA3 1mg/l was used. The explants derived from in vitro grown seedlingexplants were (shoot, epicotyl, hypocotyl) inoculated on 8 different media combination.The highest survival percentage was recorded in treatment MS + BAP 2mg/L + NAA 0.4 + IBA 0.4% in case of shoot (91.66%), epicotyls (50%), and hypocotyl (33.33%). shoot explants shoot performed better followed by epicotyls and hypocotyl. The in vitro germinated seedlings are transfer on media combinations of BAP 1mg/l with IBA 0.2, 0.4 and IAA 0.4, 0.4. The highest percent of survival of seedling was observed on ½ MS liquid, MS + BAP 1 mg/l + IBA 0.2 mg/l and MS + BAP 1 mg/l + IBA 0.4 mg/l and MS + BAP 1 mg/l + IBA 0.4 mg/l (100 percent.)ThesisItem Open Access MORPHOLOGICAL CHARACTERIZATION OF JAVA CITRONELLA VARIANTS AND ENCAPSULATION OF OIL FOR SLOW RELEASE.(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola., 2017-10-10) GANGURDE, ANUJA SUKLAL.; Akhare, Prof. A. A.The present investigation entitled “Morphological characterization of java citronella variants and encapsulation of oil for slow release” was carried out at Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola in academic year 2016-2017. Morphological characterization of five selected variants of in vitro mutagenized plants along with 4 promising control genotypes of java citronella i.e Bio-13, jalpallavi, medini and mandakini was done. On the basis of various qualitative character such as plant height(cm), number of tillers, leaf width (cm), leaf blade length (cm), yield parameter such as oil yield (100g/ml), biomass (g), morphological characters such as steam colour characterization was done. All the five variants of in vitro mutagenized plants have performed well for characters like plant height, number of tillers, leaf blade length and biomass but the variant 4 and 5 performed best over control genotypes for the character such as for plant height variant 4 (118.40) cm and variant 5 (113.60) cm, number of tillers (59.40),(61.93), leaf blade length (88.86) cm, (90.73) cm, Biomass (4204.47)g, (4526.69)g. While for characters like leaf width the variants were small as compared to control Bio-13 (2.0) cm, jalpallavi (1.78)cm High oil yield was recorded in variant 4, 5 (1.38), (1.40), Bio-13 (1.45), jalpallavi (1.42) 100g/ml. steam colour had shown variation from as green, light green, green brown, dark brown in variants and control genotypes respectively. Qualitative determination of oil constituents by thin layer chromatography was done by using different solvent system but among that Hexane : Ethyl acetate (80:20 v/v) has given best separation and identification of components .The components identified using this solvent system were geraniol with Rf value (0.77), (-)- citronellal (0.98), β-citronellol (0.80), (-)-β citronellol (0.81) with Greenish blue, Dark violet, Faint blue colour were obtained. The Oil in water (o/w) type chitosan encapsulated volatile Citronella Oil microcapsules were prepared with various concentration of chitosan ranging from (0.5 percent to 1.5 percent) and NaOH concentration ranging from (0.5 percent to 1.5 percent). The significant formlulation of microcapsules were obtained at 1.5 percent chitosan and 1 percent NaOH.The encapsulation effeciency of 95.61 percent was observed with storage stability upto 25 days.ThesisItem Open Access IN VITRO SHOOT TIP GRAFTING IN CITRUS FOR QUALITY SEEDLING PRODUCTION.(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-10-10) MALKOTE, MANIK MADHAVRAO.; Akhare, Dr. A. A.The present investigation entitled “In Vitro Shoot tip grafting in citrus for quality seedling production” was conducted at Centre of Excellence In Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist.Akola (M.S.) during the academic year 2015-2017 with the objectives to study the effect of different parameters influencing shoot tip grafting efficiency and efficacy. The experimental material of present investigation comprised the three varieties of citrus viz., Rangpur lime, Jambhiri as rootstocks and Sweet orange as scion. For micropropagation of Sweet orange field collected nodal explants and epycotyle segments from in vitro grown seedlings was used. The multiples obtained were then used for grafting purpose on Jambhiri and Rangpur lime. When seeds of Jambhiri, Rangpur lime and Sweet orange were inoculated for germination on various media combinations, The highest % of germination was found on MS media suplimented with 6% sucrose along with GA3 @ 1mg/l. The highest percent germination in case of Jambhiri and Sweet orange was 86.66% and in case of Rangpur the precent germination was 80.33%. For the preparation of scion, micropropagation of Sweet orange explants viz. nodal explants from field and epicotyls segment from in vitro germinated seedlings were tried on various concentrations of BAP @ 1, 2, 3 mg/l and NAA @ 0.5 and 1 mg/l alone or in combinations. In case of field collected nodal explants the highest success percentage was 66.66% in 6 days where as vitro derived explants respond best on media combination MS + BAP @ 3mg/l in 6.33 days. In order to optimize shoot tip grafting protocol various parameters such as sucrose concentration, age of rootstocks effect of PGRs, size of scion etc. were standardized. Amongst which sucrose % from 1.5 to 6% were tried, out of which 6% sucrose showed highest number of successful grafts on both the rootstocks Rangpur lime and Jambhiri. The rootstock age was tried from 5 to 25 days after germination and highest % success 71.42% and 62.50% in Jambhiri and Rangpur respectively was recorded when 15 days old rootstocks was used. Different PGRs viz. kinetin (1 mg/l and 5 mg/l) and BAP (1mg/l and 5mg/l) were tried in order to enhance graft union and scion growth, the highest % success of graft was noted when scion were treated with BAP @ 1mg/l prior to graft it on rootstock either Rangpur lime (66.66%) Jambhiri (73.33%). The scion size tried varies from 0.2 to 0.7 cm amongst which the highest % of success was shown by 0.6 to 0.7 mm scions. In case of Rangpur lime the % success of graft was 58.33% where as 8.033% success was recorded in Jambhiri rootstocks.ThesisItem Open Access ANALYSIS OF DNA METHYLATION PATTERN ASSOCIATED WITH FLORAL BUD DISTORTION IN SOYBEAN (Gylcine max L.).(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-08-28) KAD, MISS. SNEHAL KRISHNA; Jadhav, Dr. P. V.The present investigation entitled, “Analysis of DNA methylation pattern associated with floral bud distortion in soybean (Glycine max L.)” was carried out during 2016-17 at Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola to investigate methylation pattern associated with floral bud distortion in soybean. DNA methylation is known to play an important role in regulating gene expression during biological development and tissue differentiation in eukaryotes. In the present attempt, cytosine methylation profile was determined in elite soybean (Glycine max L.) cultivar JS-335. Methylation-sensitive amplified polymorphism was used to generate genome-wide methylation profiles and compared the patterns of cytosine methylation in floral bud distorted and asymptomatic plant. It has been learned that soybean plant is affected by the disorder known as floral bud distortion (FBD), no pod formation take place at R6 stage which results in huge yield loss in symptomatic plant(s). The methylation-sensitive amplified polymorphism technique (MSAP) which is a modification of amplified fragment length polymorphism (AFLP) used to investigate the epigenetic changes associated with FBD in soybean. The samples of genomic DNA were double-digested with either HpaII or MspI and with EcoRI. To analyze epigenetic changes, 25 MSAP primer combinations detected a few DNA methylation patterns in the plant tissue of symptomatic and asymptomatic plant. A total of 378 and 392 clear and reproducible bands were amplified from symptomatic and asymptomatic samples, respectively. In the symptomatic plant, out of 378 MSAP sites, 273 (72.22%) were found un-methylated, 31 (8.20%) were hemi-methylated, 35 (9.25%) were fully methylated; and 39 (10.31%) hyper-methylated sites were amplified. Whereas, in asymptomatic plant, out of 392 MSAP sites, 315 (80.35%) found un-methylated, 21(5.35%) hemi-methylated, 29 (7.39%) fully methylated and 25 (60.37) hyper-methylated sites were amplified. An increased level of methylation was recorded in symptomatic plant (27.28%) than that of asymptomatic (19.13%). In fact, there was evidence of increase in fully methylated ratio to 19.57% in symptomatic plant and that in case of asymptomatic revealed decrease of 12.75%. These results indicated that there were greater differences of the genomic DNA methylation extent and also variation in the pattern of methylation between the symptomatic and asymptomatic plant. It revealed that the methylation status varied substantially during the development stage of flower. The polymorphic DNA methylation fragments that showed polymorphic bands were excised from Polyacrylamide gel and cloned in vector pTZ57R/T. However, total 10 polymorphic bands were excised varying from 150 to 250 bp, out of which only 2 bands were successfully purified from the PAGE. Further these methylated fragments were cloned in vector pTZ57R/T (2886 bp). These findings successfully generated extent and pattern of cytosine methylation in floral bud distorted and asymptomatic plants. In the present investigation, although limited numbers of methylation sensitive polymorphic fragments were obtained this helped to calculate the extent and pattern of methylation associated with floral bud distortion in soybean.