MORPHOLOGICAL CHARACTERIZATION OF JAVA CITRONELLA VARIANTS AND ENCAPSULATION OF OIL FOR SLOW RELEASE.
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Date
2017-10-10
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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola.
Abstract
The present investigation entitled “Morphological characterization of java citronella variants and encapsulation of oil for slow release” was carried out at Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola in academic year 2016-2017.
Morphological characterization of five selected variants of in vitro mutagenized plants along with 4 promising control genotypes of java citronella i.e Bio-13, jalpallavi, medini and mandakini was done. On the basis of various qualitative character such as plant height(cm), number of tillers, leaf width (cm), leaf blade length (cm), yield parameter such as oil yield (100g/ml), biomass (g), morphological characters such as steam colour characterization was done. All the five variants of in vitro mutagenized plants have performed well for characters like plant height, number of tillers, leaf blade length and biomass but the variant 4 and 5 performed best over control genotypes for the character such as for plant height variant 4 (118.40) cm and variant 5 (113.60) cm, number of tillers (59.40),(61.93), leaf blade length (88.86) cm, (90.73) cm, Biomass (4204.47)g, (4526.69)g. While for characters like leaf width the variants were small as compared to control Bio-13 (2.0) cm, jalpallavi (1.78)cm High oil yield was recorded in variant 4, 5 (1.38), (1.40), Bio-13 (1.45), jalpallavi (1.42) 100g/ml. steam colour had shown variation from as green, light green, green brown, dark brown in variants and control genotypes respectively.
Qualitative determination of oil constituents by thin layer chromatography was done by using different solvent system but among that Hexane : Ethyl acetate (80:20 v/v) has given best separation and identification of components .The components identified using this solvent system were geraniol with Rf value (0.77), (-)- citronellal (0.98), β-citronellol (0.80), (-)-β citronellol (0.81) with Greenish blue, Dark violet, Faint blue colour were obtained.
The Oil in water (o/w) type chitosan encapsulated volatile Citronella Oil microcapsules were prepared with various concentration of chitosan ranging from (0.5 percent to 1.5 percent) and NaOH concentration ranging from (0.5 percent to 1.5 percent). The significant formlulation of microcapsules were obtained at 1.5 percent chitosan and 1 percent NaOH.The encapsulation effeciency of 95.61 percent was observed with storage stability upto 25 days.
Description
A field trial was conducted at Centre of Excellence in Plant Biotechnology, Dr.Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra during 2015-17.The experiment was laid out in Randomized Block Design (RBD). The healthy slips of invitro mutagenized varaints and the slips of promising genotypes (Bio-13, Jallpallavi, Mandakini and Medini) were transplanted. Observations were recorded for plant height (cm), no of tillers, leaf width(cm), four month biomass (g), oil yield(g/ml), type of growth, steam colour.
Oil components analysis was done using thin layer chromatography, for the analysis various solvent systems were used. Formation of microcapsules and analysis for the slow release effect with interval of time and the storage stability was checked.
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Citation
GANGURDE, ANUJA. (2017). Morphological characterization of java citronella variants and encapsulation of oil for slow release. Department of Agriculture Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2017, print, x, 72p. (Unpublished).