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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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2012 - 201744
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Subject: Plant Pathology × End date: 2017 × Start date: 2012 × Thesis Type: M.Sc ×
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Now showing 1 - 9 of 44
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    ThesisItemOpen Access
    STUDIES ON Macrophomina phaseolina (Tassi) Goid CAUSING DRY ROOT ROT DISEASE OF CHICKPEA {Cicer arietinum L.)
    (University of Agricultural Sciences GKVK, Banglore, 2012-07-19) GOWDRA, NAGAMMA; M. SAIFULLA
    No Abstract
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    ThesisItemOpen Access
    STUDIES ON SOYBEAN MOSAIC VIRUS DISEASE
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-12-27) MAHIPALREDDY; PRAMEELA, H. A.
    Soybean mosaic virus (SMV) disease caused by a potyvirus is a major constraint in many soybean producing areas. Soybean cv. JS-335 showed 84.00 to 96.00 per cent virus transmission upon mechanical inoculation. The virus was found to be highly stable at pH 6.0. Addition of 0.5 per cent sodium sulphite to phosphate buffer resulted in maximum transmission of virus. Seed transmission of SMV varied from five to ten per cent. Aphid, Myzus persicae Sulz. transmitted the virus from 45.00 to 60.00 per cent. The optimum preacquisition starvation period was one hour, at which 40.00 per cent virus transmission observed. A single viruliferous aphid was able to transmit the virus. The optimum acquisition access period was one minute that resulted in 40.00 per cent transmission. The optimum inoculation access period was 30 seconds at which 50 per cent transmission occurred. In the host range studies soybean, field bean, french bean, cowpea, cucumber, Datura took SMV infection and exhibited mosaic symptoms, whereas Chenopodium exhibited chlorotic local lesions. The virus was detected by DAC-ELISA using potyvirus specific antisera. The RT-PCR product of 500 bp corresponding to coat protein (CP) genes of the virus was amplified from total RNA obtained from SMV infected leaf sample. Of the 500 soybean genotypes screened against SMV under field conditions, none of the genotypes were found immune to SMV disease however, 45 genotypes were resistant and 341 were moderately resistant to soybean mosaic virus disease.
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    ThesisItemOpen Access
    STUDIES ON MANAGEMENT OF LATE BLIGHT DISEASE OF POTATO CAUSED BY Phytophthora infestans (Mont.) de Bary.
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-12-15) HARISHA, Y.N.; Nagaraj, M. S.
    Potato (Solanum tuberosum L.) popularly known as the King of Vegetables is a starchy, tuberous crop, it belongs to family Solanaceae. It is originated from South America, in the mountains of southern Peru and Bolivia. It is the world's fourth-largest food crop following rice, wheat and maize. United Nations (UN) declared the year 2008 as the International Year of Potato (IYP) in order to increase awareness about the importance of the potato in addressing issues of global concern, including hunger, poverty and threats to the environment. UN declaration of the International Year of Potato reflects the importance of the potato in the diet of the world’s population (Gupta and Modgil, 2008). Potato cultivation, transportation, storage and processing provide much needed employment in rural/sub-urban areas in India (Pandey and Sarkar, 2005).
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    ThesisItemOpen Access
    STUDIES ON Alternaria BLIGHT OF SUNFLOWER
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-23) ABHILASH; Karuna, K.
    Sunflower is an important oilseed crop of India. The crop suffers from many diseases of which Alternaria leaf blight is important. Alternaria helianthi was isolated from the leaf samples collected during kharif, rabi and summer. In all the three seasons mixed infection of A. helianthi and A. alternata was observed. Cultural studies revealed that highest radial growth was observed on Richard’s and potato dextrose agar, but profuse sporulation was in host leaf extract + sucrose and host leaf agar. Maltose and potassium nitrate were the best carbon and nitrogen sources respectively that showed maximum growth. Profuse sporulation was noticed in case of sucrose and glucose as carbon sources whereas potassium nitrate was ideal nitrogen source. Physiological studies revealed 25oC as the optimum temperature for growth whereas slightly acidic pH (6-7) showed maximum mycelial dry weight of A. helianthi. The PCR amplification and sequencing of ITS rDNA region confirmed both A. helianthi and A. alternata as the cause of leaf blight. Among the combi fungicides Propiconazole + Difenoconazole at 0.1 per cent; systemic fungicide Azoxystrobin and Propiconazole at 0.075 and 0.1 per cent; nano molecules silver colloidal particles at 250 ppm were found to be superior in inhibiting growth. Screening of nine AHT and seventeen IHT coordinated trial entries of sunflower genotypes against Alternaria helianthi under natural field conditions revealed that, seven entries were moderately resistant while all other entries were found susceptible to Alternaria leaf blight.
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    ThesisItemOpen Access
    STUDIES ON ETIOLOGY AND MANAGEMENT OF BACTERIAL SOFT ROT OF CARROT
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-16) CHANDRASHEKAR, B. S.; PRASANNA KUMAR, M. K.
    Bacterial soft rot is one of the major constraint in the production of carrot. The present study describes the first report of a new pathogen on carrot causing bacterial soft rot. Ten isolates were collected from Kolar, Chikkaballapura and Bengaluru rural districts and pathogenicity was proved on carrot. All the ten isolates were able to infect fruits of potato, tomato, chili and bell pepper in laboratory condition. In glass house condition the ten isolates caused seedling symptom on cucumber, eggplant and radish indicating wide host range of bacteria. The ten isolates were identified as Klebsiella variicola by 16S rRNA sequencing. This is the first new report of bacterial soft rot carrot caused by K. variicola. Bacillus velezensis strains A6 and P42 the novel biocontrol agent showed maximum inhibition against KV2 (24.44 %) and KV1 (26.94 %) respectively. The strains A6 and P42 were highly effective and reduced the infection of soft rot disease in both glass house (80.00 & 77.77 % respectively) and in field condition (4.33 & 4.85 % disease incidence respectively). The two strains possessed antimicrobial genes like Fengycin (269bp), Bacillomycin (370 bp), Iturin (423 bp), Surfactin (201 bp), Bacilysin (498 bp) and subtilin (375 bp). The whole genome sequencing of A6 strain revealed the genome size of 4.23 Mb and 4303 protein-coding genes were predicted. The metabolite extracts of A6 and P42 showed maximum inhibition against KV8 (18.61 %) and KV8 (17.50 %) respectively. The GCMS analysis of culture filtrate revealed ten bioactive compounds from A6 and six from P42 after derivatization of the culture filtrate, whole nonderivatization culture filtrate of A6 and P42 identified nine and twelve bioactive compounds.
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    ThesisItemOpen Access
    INVESTIGATION ON DOLICHOS MOSAIC VIRUS DISEASE ON FIELD BEAN
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-16) ANANDA BHEEMASHANKAR, AVARAD; Ramappa, H. K.
    Dolichos mosaic virus disease on field bean is caused by a potyvirus is characterised by the symptoms like vein clearing, uneven leaf lamina, twisting of leaves, mosaic mottling and blistering on newly formed trifoliate leaves. The incidence of the disease in the Southern Karnataka ranged from 16.01 per cent to 26.98 per cent. The highest disease incidence was recorded in Ramanagara (26.98 per cent) and lowest disease incidence in Kolar (16.01 per cent) during 2016. Five hundred and forty five genotypes screened under field condition against dolichos mosaic virus disease. Among 545 genotypes, 41 genotypes showed resistant reaction, 377 genotypes (HA-3 and Kadale avare) were showed moderately resistant reaction with the range of 6.98 to 14.49 per cent disease incidence and 100 genotypes involving popular variety HA-4 were showed moderately susceptible reaction with disease incidence range of 16.56 to 24.22 per cent. Sixteen and eleven genotypes were showed susceptible and highly susceptible reaction respectively. Barrier crop maize + three sprays of Imidacloprid 17.8 % SL@ 0.5 ml/L at 20, 30 and 40 DAS or three sprays of Imidacloprid 17.8 % SL @ 0.5 ml/L alone at 20, 30 and 40 DAS were found effective in reducing the incidence of dolichos mosaic virus, its vector and increasing grain yield followed by three sprays of 3 % Mineral oil at 20, 30 and 40 DAS was also found effective in management of dolichos mosaic virus disease, its vector (Aphis craccivora) and increasing grain yield.
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    ThesisItemOpen Access
    BIOMANAGEMENT OF PHYTOPHTHORA COLLAR ROT AND BLIGHT OF CAPSICUM
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-11) NEETA N., PATIL; Narendrappa, T.
    Bell pepper, an important vegetable crop, affected by destructive soil borne pathogen Phytophthora causing collar rot and blight. The pathogen was identified as P. capsici based on microscopic observations of mycelia and sporangia characters like coenocytic, finely granular mycelia with hyphal swellings and pappilate sporangia attached to a long pedicel. The pathogen produced different symptoms like root rot, damping off, collar rot and fruit rot on capsicum. Aswell, pumpkin, bottle gourd and bush squash were also infected by P. capsici. The Carrot agar media supported highest radial growth of the pathogen. Trichoderma viride (Tv-M), T. harzianum (Th-58) and Bacillus velenzensis (P-42) inhibited the pathogen by 46.06, 44.85 and 31.11 per cent respectively under laboratory condition. The culture filtrate of T. harzianum (Th-16) and volatile compounds produced by T. viride (Tv-16) inhibited the pathogen by 46.41 and 36.67 per cent respectively. Neem leaf extract significantly inhibited the growth of P. capsici (78.96 %) followed by ginger (73.78 %), garlic (68.59 %) and turmeric (65.11 %). The essential oil of lemongrass showed maximum inhibition of 90.52 per cent followed by citronella, lavender and patchouli with 84.42, 36.57 and 31.15 per cent with Minimum Inhibitory Concentration (MIC) of 150 ppm, 250 ppm, 300 ppm and 300 ppm respectively. The essential oil showed degenerative changes in the hyphal morphology of the pathogen. Application of the bioagents T. viride-M, B. subtilis (P-21) and B. velenzensis (P-42) recorded least disease severity of 55.00, 55.00 and 51.67 per cent respectively compared to untreated control under glasshouse condition.
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    ThesisItemOpen Access
    DECODING THE ENDOPHYTES ASSOCIATED WITH FINGER MILLET BLAST RESISTANT AND SUSCEPTIBLE GENOTYPES BY WHOLE GENOME METAGENOME SEQUENCING
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-11) RADHIKA, U. DESAI; Prasanna Kumar, M. K.
    Finger millet harbors many beneficial endophytes that contributes to plant growth promotion and protection against pathogens. The present study describes the first reported data regarding whole genome metagenomic analysis on the diversity of endophytes associated with two finger millet varieties viz., GPU-28 (resistant) and Udurumallige (susceptible) using Illumina HiSeq platform. A total of 4,307,574 in GPU- 28 and 4,438,968 reads in Udurumallige were generated and assembled using Ray Meta assembler. MetaGeneAnnotator (MGA) was used to predict open reading frames (ORFs) and searched against the non-redundant (NCBI-nr) database using DIAMOND software to find out protein homology. The filtered ORFs revealed the presence of actinobacteria and proteobacteria as the major phyla in both the varieties. Based on our preliminary analyses, GPU-28 had more diverse species of endophytes as compared to Udurumallige. Functional annotation revealed that the genes related to stress response and metabolism of aromatic compounds were higher in GPU-28. Out of 99 endophytes isolated the highest number of endophytes were isolated from leaf (n=29), stem of GPU-28 (n=18) followed by root of Udurumallige (n=18). The genus Enterobacter (32.96 %) in GPU-28 and Bacillus (26.37 %) in Udurumallige found most dominant endophytes. In-vitro evaluation of endophytes against Magnoporthe grisea found that Bacillus licheniformis strain GPUL23 showed the highest per cent inhibition of 66.67. In In-planta tests against M. grisea, Paenibacillus polymyxa strain GPUS-8 showed effective in decreasing per cent disease severity of 16.67 and 18.89 in GPU-28 and Udurumallige, respectively. These investigations support that the endophytes play a vital role in providing resistance to blast disease in GPU-28.
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    ThesisItemOpen Access
    FORMULATIONS AND DELIVERY METHODS OF Bacillus velezensis STRAIN B13 IN BIOMANAGEMENT OF FINGER MILLET BLAST
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-08-05) HEMAVATHI, M. C.; Nagaraja, A.
    Finger millet (Eleusine coracana (L.) Gaertn.), one of the oldest crops in India is referred as “nrttakondaka” in the ancient Indian Sanskrit literature, which means “Dancing grain,” was also addressed as “rajika” or “markataka” (Achaya, 2009) is the main staple food of millions of people in India and Africa. Finger millet is the main dietary component in southern Karnataka and is valuable as it contains the amino acid methionine, which is lacking in the diets of hundreds of millions of the poor who live on starchy staples such as cassava, plantain, polished rice or maize meal. Finger millet can be ground and cooked into cakes, puddings or porridge. In India, finger millet has been grown over an area of 12.08 million hectares and production of 20.60 million tons with 1706 kg ha-1 productivity (Anon., 2014). Major ragi growing states are Karnataka, Maharashtra, Uttarakhand, Tamil Nadu, Odisha, Andhra Pradesh, Gujarat, Jharkhand, West Bengal