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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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2009 - 200912010 - 20141
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Subject: Plant Pathology × Author: BASAVARAJ, S. × Start date: 2000 × Type: Thesis ×
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    ThesisItemOpen Access
    SURVEY, MOLECULAR DETECTION AND PARTIAL CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS (CMV) AND BANANA BRACT MOSAIC VIRUS (BBrMV) ISOLATES INFECTING BANANA IN KARNATAKA
    (University of Agricultural Sciences, Bangalore, 2014-09-12) BASAVARAJ, S.; Rangaswamy, K. T.
    The use of tissue culture planting material has helped to a greater extent to overcome the problems of viral diseases. However, the genetic variant associated with tissue culture plants has limited their use for raising the crop. Eighty five tissue cultured and sucker propagated banana gardens were surveyed for banana viruses and tissue culture variants. The mean incidence of virus diseases was high (3.78%) in gardens established using suckers compared to tissue culture established gardens (1.47%). The average incidence of somaclonal variants was 2.95 % in tissue cultured gardens. Out of 5532 samples diagnosed only 0.7% of the samples were found positive for viruses. Out of 3572, samples tested for genetic purity using ISSR markers, only 0.1% samples were found to not true to type. Novel, rapid and cost effective Loop mediated isothermal amplification (LAMP) based techniques were revalidated for detection of BBTV, BSV, CMV and BBrMV. Colorimetric indicator dyes like HNB, Calcein, SYBR Green I and thiazole orange successfully differentiated healthy and virus infected samples after amplification, thus colorimetric detection was standardised for detection of banana viruses. Out of 25 ISSR markers validated for their suitability to detect the most common tissue culture variants only three ISSR markers detected the variant with chimeric leaves. However, failed to detect other types of variants observed during survey. RT-PCR amplification of the CMV and BBrMV CP genes using gene specific primers resulted in the amplification of ~650 bp and ~1062 bp products respectively. Phylogenetic analysis of banana CMV and BBrMV isolates under present study based on both nucleotide and amino acid sequences of CP revealed that the CMV isolate belongs to subgroup IB and BBrMV isolate belongs to BBrMV Karnataka group.
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    ThesisItemOpen Access
    MOLECULAR DETECTION OF SUGARCANE YELLOW LEAF VIRUS AND FIELD REACTION OF SUGARCANE GENOTYPES FOR THEIR RESISTANCE
    (University of Agricultural Sciences, Bangalore, 2009-07-15) BASAVARAJ, S.; PRAMEELA, A.
    Survey conducted to assess the incidence of yellow leaf disease of sugarcane in Cauvery command area of Southern Karnataka indicated the occurrence of disease incidence up to 35.3 per cent. Among the three districts, highest disease incidence of 35.3 per cent was in Mandya district and lowest incidence was recorded in Chamarajnagar district (10.5 %). A fluorescent microscopic technique using aniline blue dye was standardized for detection of Sugarcane yellow leaf disease (ScYLD). High intensity of fluorescence in phloem region of infected compared to healthy plants was observed with aniline blue stain. The disease was successfully detected by Reverse transcription polymerase chain reaction (RT-PCR) with specific oligonucleotide primers. A distinct band at 320 bp was obtained from ScYLD infected plant samples but not from RNA extracted from healthy plant samples. Among 45 genotypes screened under natural conditions, 15 genotypes namely Co95005, CoC90063, CoVC93136, Co99006, Co99008, Co86249, Co95012, Co62175, Co03632, CoSnk.03754, Co85004, C094008, CoVC2003-165, Co0416 and CoSnk.3822 showed resistant reaction to the disease. While, 8 genotypes showed moderately resistant; 10 genotypes showed moderately susceptible and 12 genotypes showed susceptible reaction to the disease. The most popular cultivar Co86032 was found comparatively susceptible to the disease than other cultivars grown in the command area. The level of sucrose per cent, commercial cane sugar per cent and purity per cent in ScYLD infected canes was lower compared to the healthy canes. Physical methods viz., hot water treatment, hot air oven treatment and aerated steam therapy were not effective in eradicating the causal agent.