Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

20091
Reset filters
Subject: Plant Biotechnology × Author: AYEESHA, MUNAWERY × End date: 2009 × Start date: 2006 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF YEAST STRAINS FOR EFFICIENT ETHANOL PRODUCTION USING SSR MARKERS
    (University of Agricultural Sciences, Bangalore, 2009-07-15) AYEESHA, MUNAWERY; HARINIKUMAR, K.M.
    The study was carried out to isolate yeast strains from their natural habitat and to screen them for ethanol tolerance. A total of 45 yeast strains were isolated from sugar rich sources. Twelve were identified as Saccharomyces spp. Based on colony type and cell morphological characters such as cell shape and budding characters. Saccharomyces spp. were screened for the ability to tolerate different ethanol concentrations from 5-15% growth in different ethanol concentrations varied from one isolate to another. Yeast isolates showed tolerance level from 5-15%. The best strain had 15% ethanol tolerance strain YDE, which showed high tolerance to ethanol and YMS with low tolerance were mutated by UV radiations with time intervals of 1, 3 and 5 mins and through chemical method using Acridine orange and Ethidium bromide at concentrations of 0.25, 0.50. 0.75 and 1.00 g/ml and were subjected to screening under same ethanol concentrations from 5-15%. Mutated isolates showed decreased growth and tolerance under high ethanol stress compared to their original isolates. SSR profiling was employed to characterize yeast isolates. Eleven Saccharomyces spp. were selected and were subjected for SSR analysis using 10 yeast specific SSR primers selected from public domain and constructed by chromos Biotech Company. Showed polymorphism among the isolates. Dendrogram was constructed according to unweighted pair group arithmetic means using STATISTICA software. Cluster analysis revealed major two groups, two isolates formed one group and other nine isolates formed other group. There was no correlation between SSR profiling and physiological screening of yeast isolates for ethanol tolerance.