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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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2013 - 20154
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Subject: Biotechnology × End date: 2017 × Start date: 2012 ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    BIOCHEMICAL STUDIES ON RICE {Oryza sativa L.) CULTIVARS FOR NUTRITIONAL EVALUATION
    (University of Agricultural Sciences GKVK, Banglore, 2013-03-05) R. DRUVASHREE; R. CHANDRU
    Rice cultivation is improving in India with the release of number of varieties and hybrids. An investigation was undertaken to evaluate the physico chemical properties, biochemical composition and nutritional qualities of selected eight rice varieties viz., BPT-5204, IR-64, Jyothi, KRH-2, MAS-946, Thanu, Karimundga and KRH-4. Rice cultivars differed significantly in respect of milling characteristics which is reflected by the significant differences of the rice cultivars with respect physical characteristics like length, breadth, test weight, chalkiness etc. Rice varieties Thanu and Jyothi had significantly lesser cooking time (20 minutes) compared to all other varieties/hybrids which took 25 minutes for cooking. The rice cultivars differed significantly with respect to swelling ratio by weight and it ranged from 3.73 to 4.41, hybrids such as KRH-2 and KRH-4 had higher swelling ratio by weight. Physical characteristics of grains, physicochemical and functional properties of flour was estimated. The investigation revealed variations in most of the parameters studied. Variation in size of the grain, weight and density was evident. Oil absorption capacity was highest in KRH-2 (2.1%) and hydration capacity was highest in Sharada (0.9%). The crude fat content varied from 0.27-1.67 per cent. BPT-5204 was found to have high protein about 8.27%. Studies on in vitro protein digestibility revealed highest digestion in BPT-5204 (92.91%) and least digestion in KRH-2 (71.1%). Starch content varied between 42.61 to 55.98% and amylose content varied between 29.23 to 38.1%. Starch digestibility was highest in Karimundga (47.44%) and least in Sharada (31.39%).
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    ThesisItemOpen Access
    MORPHOLOGICAL AND MOLECULAR EVALUATION OF POLE AND BUSH TYPE FRENCH BEAN (Phaseolus vulgaris L.) ACCESSIONS FOR IRON AND ZINC CONTENT
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-09-23) NAYANA, K. N.
    French bean or common bean is one of the nutritionally important leguminous crops grown for the tender pods and seeds. It is a major source of dietary proteins, carbohydrates, fibre and micronutrients. French bean seeds exhibit variability for seed mineral accumulation, particularly seed Fe and Zn, thus the present study was carried out. Fourty four accessions were grown in field condition and various morphological traits were studied and the Fe and Zn content were estimated through ICP-OE Spectroscopy. The studies revealed that the pole type accessions exhibited slightly higher mean Fe and Zn content over bush types. The pole type accessions IC318852 (203.66 mg Kg-1) and IC319827 (195.20 mg Kg-1) showed higher Fe content and L line (69.40 mg Kg-1) and EC500745 (67.80 mg Kg-1) showed higher Zn content. The above accessions were screened using SSR markers linked to Fe and Zn content, nine SSR primers amplified, five markers were linked to seed Fe and Zn content (BMd16, BMd33, BMd47, BM139 and BM181) and were monomorphic. However, the other three markers specific to Fe content (BM211, BM170 and BMd160) and one marker was specific to Zn content (BM154) were amplified only in selected accessions, indicating larger polymorphism among the French bean accessions. The above SSR markers can be used in future crop improvement programmes involving biofortification of French bean for Fe and Zn content.
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    ThesisItemOpen Access
    DOCUMENTATION AND MOLECULAR CHARACTERIZATION OF MUSHROOMS IN GKVK CAMPUS, UNIVERSITY OF AGRICULTURAL SCIENCES, BENGALURU, KARNATAKA
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-07-10) SANDHYA, M. C; Eranna, R L
    Mushrooms are epigeous and hypogeous fleshy fruiting bodies of fungi belonging to Basidiomycetes and certain Ascomycetes. Gandhi Krishi Vigyana Kendra (GKVK) campus of University of Agricultural Sciences (UAS) Bengaluru, provides congenial climate for the growth and development of variety of mushroom species. In the present study, twenty one mushroom species were documented from the campus during rainy season (July-October-2014). While collecting the mushrooms, the field information such as date, abundance, growth habit, substrate and location were recorded. The collected mushrooms were identified by ITS region sequence homology using National Centre for Biotechnology Information (NCBI) data base. The sequences of the collected mushrooms when compared with NCBI data base revealed 87-100 per cent homology. The mushrooms identified based on sequence homology are Macrolepiota globosa (GKVK-1), Ganoderma australe (GKVK-2), Lepista sp. (GKVK-3), Phlebopus portentosus (GKVK-4), Termitomyces sp. (GKVK-5), Agrocybe pediades (GKVK-6), Leucoagaricus crystallifer (GKVK-7), Podoscypha petalodes (GKVK-8), Agaricus sp. (GKVK-9), Tricholoma giganteum, (GKVK-10), Coprinellus disseminates (GKVK-11), Ompholotus olivascens (GKVK-12), Agaricus sp. (GKVK-13), Macrolepiota dolochula (GKVK-14), Panus conchatus (GKVK-15), Marasmius leveilleanus (GKVK-16), Polyporus arcularius (GKVK-17), Lepiota fuscovinacea (GKVK-18), Agrocybe semiorbicularis (GKVK-19) and Marasmius sp. (GKVK-20). The mushroom designated as GKVK-21 was identified based on its phenotypic characters as Ganoderma lucidum which is a medicinal mushroom.
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    ThesisItemOpen Access
    STANDARDIZATION OF TRANSFORMATION PROTOCOL AND EXPRESSION OF HEPATITIS B SURFACE ANTIGEN (HBsAg ) IN Coleus forskholii
    (University of Agricultural Sciences GKVK, Bangalore, 2013-08-27) DESHMUKH SNEHAL, SANJAYRAO; RAMANJINI GOWDA, P H
    Hepatitis B Virus (HBV) infection remains a major worldwide infectious disease with serious long-term morbidity and mortality. It is estimated that two billion people have been infected with the virus, and more than 360 million have chronic HBV infections. The effective way to control the virus is to take HBV vaccine. Hepatitis B surface antigen (HBsAg) is an effective protective antigen suitable for vaccine development for prevention of this disease. Hence, plant-based production of vaccine for hepatitis B may be an economically feasible alternative. The present study lays emphasis on production of a recombinant protein/vaccine (HBsAg, Hepatitis B surface antigen) in Coleus forskohlii against hepatitis-B disease through Agrobacterium mediated gene transfer. MS basal media with 6mg/L BAP and 0.4mg/L IAA was effective to induce a good callus as well as multiple shoot production from leaf explant. The shoots were also rooted on same MS basal media. The control of Agrobacterium overgrowth was effective in OD dilution of 1:40 and 1:50with OD value of 0.6, immersion time of 2 minutes and co-cultivation period of one day. The transformed plants were confirmed by Polymerase Chain Reaction (PCR) analysis which has shown a band size of 681bp. Expression of HBsAg was confirmed by Sodium Dodecyl Sulphate (SDS) and DOT-BLOT. The expected protein band size of 24 KDa on gel confirmed expression of HBsAg S-protein in transgenic plants. Since Coleus forskohlii is a medicinal plant and nature of propagation is vegetative, it is advantageous to produce recombinant HBsAg S-protein in this plant.