MORPHOLOGICAL AND MOLECULAR EVALUATION OF POLE AND BUSH TYPE FRENCH BEAN (Phaseolus vulgaris L.) ACCESSIONS FOR IRON AND ZINC CONTENT
Loading...
Files
Date
2015-09-23
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
French bean or common bean is one of the nutritionally important leguminous
crops grown for the tender pods and seeds. It is a major source of dietary proteins,
carbohydrates, fibre and micronutrients. French bean seeds exhibit variability for seed
mineral accumulation, particularly seed Fe and Zn, thus the present study was carried out.
Fourty four accessions were grown in field condition and various morphological traits
were studied and the Fe and Zn content were estimated through ICP-OE Spectroscopy.
The studies revealed that the pole type accessions exhibited slightly higher mean Fe and
Zn content over bush types. The pole type accessions IC318852 (203.66 mg Kg-1) and
IC319827 (195.20 mg Kg-1) showed higher Fe content and L line (69.40 mg Kg-1) and
EC500745 (67.80 mg Kg-1) showed higher Zn content. The above accessions were
screened using SSR markers linked to Fe and Zn content, nine SSR primers amplified,
five markers were linked to seed Fe and Zn content (BMd16, BMd33, BMd47, BM139
and BM181) and were monomorphic. However, the other three markers specific to Fe
content (BM211, BM170 and BMd160) and one marker was specific to Zn content
(BM154) were amplified only in selected accessions, indicating larger polymorphism
among the French bean accessions. The above SSR markers can be used in future crop
improvement programmes involving biofortification of French bean for Fe and Zn
content.
Description
Keywords
null