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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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2021 - 20233
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Subject: Agricultural Biotechnology × End date: 2023 × Start date: 2021 × Type: Thesis × Thesis Type: M.Sc ×
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Now showing 1 - 3 of 3
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    ThesisItemEmbargo
    DEVELOPMENT OF MYMV RESISTANCE IN GREENGRAM [Vigna radiata (L.) Wilczek] USING BULK SEGREGANT ANALYSIS
    (2023-01-20) POOJITHA; K. M. HARINIKUMAR
    Greengram (Vigna radiata (L). Wilczek) is an important pulse crop owing to its short growth duration, low water requirement, and its suitability for crop rotation and nutritional security being a rich source of protein. Greengram’s yield is greatly affected by numerous abiotic and biotic factors. Biotic stress produced by viruses especially Mungbean Yellow Mosaic Virus (MYMV) is the main constraint in mungbean production causing substantial yield reduction up to 85 per cent. In present study, 96 F2 genotypes from the cross between GG-ABL-449 x GG-ABL-265 were subjected to natural screening to assess their resistance or susceptibility against MYMV. F2 mapping population segregated in 70 susceptible: 26 resistant genotypes i.e., 3:1 ratio showing that resistance to MYMV is governed by monogenic recessive gene. Among 96 genotypes, seven were resistant, nineteen were moderately resistant, forty-one were moderately susceptible, twenty-two were susceptible and seven were highly susceptible. All the 96 genotypes were phenotypically evaluated for seven quantitative traits under field conditions. The GCV and PCV were found higher for important yield contributing traits and seed yield is positively correlated with major yield contributing traits. The parental polymorphism study showed two markers, CYR1 and VMYR1 polymorphic out of four Resistance Gene Analogue (RGA) primers linked to MYMV resistance which showed segregation in Bulk Segregant Analysis (BSA). The markers CYR1 and VMYR1 showed differences between bulks and parents with a fragment size of 445 bp and 1236 bp respectively in resistant bulks indicating that these markers are tightly linked to the MYMV resistance gene.
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    ThesisItemOpen Access
    MOLECULAR AND INSECTICIDAL CHARACTERIZATION OF NOVEL CRY TOXINS FROM Bacillus thuringiensis (BERLINER) AGAINST THE FALL ARMY WORM (Spodoptera frugiperda) AND BRINJAL ASH WEEVIL (Myllocerus subfasciatus)
    (University of Agricultural Sciences, Bangalore, 2021-12-31) ANUSHA, D; NAGESHA, S N
    Twelve Bt strains were isolated from root nodules using six approaches, viz.,analysis of crystal protein production by microscopy; detection of cry gene content by PCR, Plasmid profiling, SDS-PAGE profiling; cloning and sequencing, phylogenetic analysis; and toxicity testing. Under a light microscope, all 12 Bt strains examined were gram-positive, endospore-forming, and had normal Bt crystal structures. A bipyramidal inclusion was predominant in 42.2 %. Five distinct plasmids were observed in the present study. Universal primers and gene specific primers were used to detect Cry-type genes by the PCR. The nucleotide sequences of amplified new cry genes were BLASTed against NCBI database sequences, and they were labeled as native Bt strains with high homology (60–100%) to existing Bt strains. The nucleotide sequences of new Bt cry genes were deposited in the NCBI GenBank database. Three new cry1, cry3A and cry26 complete crygenes were isolated. Cry genes were cloned and expressed in the pRSET vector. The inferred 3-D structural model of the novel cry gene, which was predicted using Phyre2 homology modeling, demonstrates that the gene has three domains that participate in the development of a pore and define the receptor's binding selectivity. The new Bt strains were highly pathogenic, with pathogenicity ranging from 93 to 100 percent against Spodoptera frugiperda larvae and Myllocerus subfasciatus adults. In conclusion, native Bt strains from root nodules were shown to have bio-insecticidal activity on larvae of S. frugiperda and adults of M. subfasciatus
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    ThesisItemOpen Access
    MOLECULAR ANALYSIS OF INTERACTION BETWEEN MICROBIAL INOCULANTS ON EARLY BLIGHT OF TOMATO AND DOWNY MILDEW OF CUCUMBER
    (University of Agricultural Sciences GKVK, Bangalore, 2021-06-30) POOJA, R; RAVISHANKAR, K. V.
    Biocontrol agents in management of plant diseases are gaining huge importance due to their advantage over chemical control methods. This study was conducted to evaluate the potential antagonistic agents against Alternaria solani (which causes the early blight of tomato) and to understand the interaction between host, pathogen and biocontrol agent. Among the different isolates studied in dual culture assay JL5 (Trichoderma longibrachiatum), GJ16B (Trichoderma harzianum), UGF (Bacillus subtilis), LA (Trichoderma pleuroticum) showed significant inhibition of A. solani. GCMS analysis revealed the presence of different classes of VOCs (Volatile organic compounds) i.e. terpenes, alcohols, esters, aldehydes, hydrocarbons and also the induction of some VOCs in the mixed culture which are absent in pure culture of pathogen and Trichoderma sp. In in-vivo assay JL5, GJ16B and LA treated plants showed reduced symptoms of early blight compare to the control. The activity of defense related enzyme i.e peroxidase (POX), superoxide dismutase (SOD) and polyphenol oxidase (PPO) found to be higher in JL5, GJ16B and UGF treated plants after inoculation of pathogen. The total phenol and flavonoid content of the plant showed increasing trend after challenging with pathogen being highest in all treated plants. Expression of defense related genes i.e. POX, PR2, PR1 increased in some treatments like JL5, UGF followed by pathogen inoculation indicating the role of the biocontrol agents in inducing the host defense response upon pathogen infection. Bioagents JL5, GJ16B and AMC were evaluated here did not show any effect on controlling downy mildew of cucumber under field condition