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Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar

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2012 - 20152
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Author: Sandeep × Thesis Type: M.V.Sc. ×
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    To study the dairy characters of lactating Murrah buffaloes on the basis of physical appearance and body parts measurements
    (LUVAS, 2015) Sandeep; Kar, Dipankar
    An experiment was undertaken to study the dairy characters of lactating Murrah buffaloes on the basis of physical appearance and body parts measurements. The objective of the study was to correlate the milk yield of Murrah buffaloes with certain body parts measurements and to find the relationship between phenotypic characters and milk yield. Seventy lactating Murrah buffaloes were selected from Buffalo Farm of LUVAS, Hisar. The buffaloes were selected at random basis in a range from first to fifth parity. Traits studied were 305 days milk yield (MY), body weight (BW),body length (BL), muzzle width (MW), lower jaw width (LJW), height at wither (HW), height at chest from ground (HCG), abdominal girth (AG), chest girth (CG), body depth fore (BDF) and body depth rear (BDR), hip bone distance (HBD), pin bone distance (PBD), skin thickness (STK), tail length (TL), tail switch length (TSL) and white switch length(WSL). The phenotypic characters studied were head type, face type, horn pattern, coat color, tail switch color, brisket distance, udder depth, teat tips, teat shape, rear attachment of udder, fore and rear udder shape and temperament. The result of the study showed that significant positive correlation found between milk yield and body weight (0.26), abdominal girth (0.64) and muzzle width (0.42). Significant negative correlation was observed between milk yield and skin thickness (-0.79). Most of the body parts measurements viz., body length, wither height, chest girth, hip and pin bone distance and rear body depth measurements were positively associated with milk yield but not up to level of significance. It can be inferred from present study that milk yield was found significantly associated with most of phenotypic characters studied in relation to head type, brisket distance, udder viz. udder depth, rear attachment, rear and fore udder shape and temperament. Present study revealed that physical appearance, viz. face type, teat tips, horn pattern and tail switch color did not influenced milk yield significantly in lactating Murrah buffaloes.
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    Development of Recombinant antigen based ELISA for Serodiagnosis of Theileria equi in equines
    (LUVAS, 2012) Sandeep; Rakha, N.K.
    The erythrocytic-stage surface protein, Equi Merozoite Antigen 1 (EMA-1), is a major candidate for the development of a immunodiagnostic antigen forTheileria equiinfection . In order to establish an effective diagnostic method for laboratory use, 547bp gene segment of EMA-1 of Theileria equi from Indian isolate was expressed in Escherichia coli as a Glutathione-S-transferase-tagged protein (GSTEMA-1) and the fusion protein was purified by Glutathione sepharose beads. The expressed fusion protein reacted with specific antibodies in Western blot and had an apparent molecular mass of 43 kDa which was largely consistent with its theoretical value. The recombinant purified GST-EMA-1 protein was used in enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. equi in horses. The ELISA clearly differentiated T. equi-infected from normal horse sera. Field serum samples collected from horses in Haryana and Uttarakhand, were examined for the determination of T. equi antibodies in ELISA. Of 240 samples analyzed, 45.41% (106/240) were positive for T. equi infection. These results suggest that the GST-EMA-1 protein expressed in E. coli could be a reliable immunodiagnostic antigen for ELISA and that T. equi infection is endemic in the areas from which samples were collected.