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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Veterinary Microbiology × Author: Lali, Kasturi × Start date: 2018 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Microbiological studies on reproductive disorders of canines
    (Palampur, 2022-12-03) Lali, Kasturi; Dhar, Prasenjit
    For centuries, dogs have been considered to be man’s best friend. Diseases related to reproductive tract are quite common in canine practice. The present study was carried out with the objective to find out the association of different microbes in reproductive disorders of canines and determination of the antimicrobial susceptibility profile of the isolated microbes. Additionally, the bacterial enumeration of the obtained samples and minimum inhibitory concentration of the antimicrobials were also determined. Molecular detection of Brucella spp. was done employing PCR. A total number of 102 samples were collected from different places of Himachal Pradesh. These were accrued from dogs [both males (11) and females (91)] suffering from any reproductive disorders (purulent vaginitis, pyometra, dystocia, abortion, orchitis, etc.) as well as from apparently healthy dogs. The samples included vaginal swabs, uterine discharge, aborted foetus, preputial swabs and washings. The most frequently recorded reproductive disorder was of purulent vaginitis (15/102; 14.71 per cent) followed by pyometra (11/102;10.78 per cent). The age group of 2-5 years were mostly affected (46.07 per cent). A total of 124 isolates were recovered from 102 samples of which 112 (90.32 per cent) were bacterial isolates and 12 (9.68 per cent) were fungal isolates. The most prevalent bacteria obtained was E.coli (21.77 per cent) followed by Staphylococcus spp. (15.32 per cent) and Pseudomonas spp. (7.3%) while the most commonly isolated yeast was Candida spp. (4.84 per cent). The antimicrobial susceptibility profile of the recovered bacterial isolates revealed enrofloxacin (94.49 per cent) to be the most effective drug while fluconazole (100 per cent) was recorded as the most effective antimycotic agent. Bacterial enumeration revealed significant difference in the number of bacteria (cfu/ml) in diseased dogs in comparison to apparently healthy dogs which makes the quantitative analysis reliable. The obtained MIC value of different antimicrobials showed correlation with their resistance pattern in the AMST. 13 out of 102 samples (12.7 per cent) were positive for the genus Brucella (bcsp31) through PCR, however no isolates were obtained through standard procedures of isolation.. In conclusion, the study recorded plethora of microbial species associated with various reproductive disorders in canines along with their antimicrobial patterns.