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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20211
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Subject: Genetics and Plant Breeding × Author: KRITESH, THAKUR × End date: 2021 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Androgenesis mediated induction of haploid from Oryza sativa L. x Oryza glaberrima Steudt. derivatives
    (Palampur, 2021-09-29) KRITESH, THAKUR; Basandrai, Daisy
    The present investigation entitled “Androgenesis mediated induction of haploid from Oryza sativa L. x Oryza glaberrima Steudt. derivatives” was conducted at Department of Genetics and Plant Breeding. Combining the best traits of O. sativa and O. glaberrima by interspecific hybridization may be harnessed to develop pre-breeding material. Keeping this in view seven diverse genotypes viz. PB-3, Pusa 1121, and Kasturi (Basmati rice), HPR 2612 (Aromatic rice ), HPR 2795 (Red rice), HPR 2373 (Normal rice) and Matali (Japonica rice) were crossed with O. glaberrima to study hybridization potential and callus induction through anther culture. Among crosses of O. sativa x O. glaberrima, the highest number of seed set and crossing percentage was observed, when pollination was done between 9 to 10 A.M. Among the genotypes, the highest crossing percentage was observed in variety HPR 2795 followed by PB-3 indicating that O. glaberrima had better chances of crossing between Indica red rice and Basmati rice as compared to Japonica rice. In anther culture studies, 2.2% callusing was observed in cold pretreated anthers as compared to 0.06% callusing in mannitol pretreated anthers indicating that cold pretreatment of anthers is more effective than mannitol treatment. Among crosses, F1’s of Matali x O. glaberrima showed the highest percent of callusing when anthers were placed in N6 and modified N6 medium. Hence, the Japonica variety showed the highest response to anther culture as compared to other genotypes. Modiefied N6 medium was found to be more suitable for callus induction. However, none of the callus regenerated into plantlets as after 4 weeks the callus turned brown and then black. Based on mean performance, highest grain yield/plant was observed in red rice variety, HPR2795 followed by HPR 2373 and among crosses, Matali x O. glaberrima showed the maximum grain yield/plant followed by HPR 2612 x O. glaberrima. High heritability coupled with high genetic advance and high PCV and GCV was recorded for iron content, magnesium content, L/B ratio and grain yield /plant indicating that there is substantial variability ensuring ample scope for improvement for these traits through selection. Grain yield/plant had positive and significant correlation with plant height, total tillers/plant, effective tillers/plant and grain breadth. Improvement in grain yield/plant can be obtained by improving these characters. On the basis of SSR markers, 12 genotypes were clustered into 2 major groups I and II comprising 5 and 7 genotypes, respectively. O. glaberrima and RB-7 were the most diverse genotypes showing genetic dissimilarity on molecular basis. So, they can be used as potential parents for crossing with other genotypes