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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the ā€œKrishikarman awardā€ of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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2012 - 20152
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Subject: Biochemistry Ɨ End date: 2015 Ɨ Start date: 2012 Ɨ Thesis Type: M.Sc Ɨ
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    ThesisItemOpen Access
    ISOLATION AND PURIFICATION OF LECTINS FROM VIGNA SPECIES
    (CSKHPKV, Palampur, 2015-07-13) Tripathi, Ankur; Katoch, Rajan
    In the present investigation, seeds of five Vigna species were investigated for hemagglutinating activity against human and rabbit erythrocytes. The seed extracts of Vigna mungo and Vigna radiata showed agglutination activity with human as well as rabbit erythrocytes, whereas Vigna unguiculata, Vigna umbellata and Vigna angularis exhibited agglutination only with trypsinized rabbit erythrocytes. Among the various tested sugars, agglutination activity was best inhibited by D-galactose. Vigna radiata extract exhibited a higher level of agglutination with rabbit erythrocytes and was selected for isolation and purification of lectin. Lectin was purified by employing a protocol that entailed ion exchange, gel filtration and affinity chromatography with 192.47 fold purification and 73.14 (HU/mg) specific activity. Under reducing conditions purified lectin was found to be a monomer with 25.0kDa molecular weight. Maximum agglutination activity of purified lectin was observed at pH 5.0 with (98%) stability and maintained steadily between pH 6-9. Maximum agglutination activity was observed at 370C (68.76%) and minimum at 650C where agglutination activity was completely lost. Agglutination activity was readily inhibited by D-galactose at 3.12mM. Lectins, due to various biological properties have a potential in pest control and plant defense. Their high level expression can confer resistance to a wide range of insects and could be exploited for crop improvement.
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    ThesisItemOpen Access
    IN VITRO STUDIES IN Cymbidium giganteum WALL. AND Rhynchostylis retusa (L.) Bl.: COMMERCIALLY IMPORTANT ORCHIDS
    (Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 2012-07) GHOSH, AMRITA; Sharma, Madhu
    Asymbiotic seed germination was optimized in Cymbidium giganteum Wall. and Rhynchostylis retusa (L.) Bl.. Three different basal media viz., Murashige and Skoog (MS; 1962), Mitra et al. (M; 1974) and Knudson ā€žCā€Ÿ (KC; 1946) were used. The first sign of seed germination was marked by swelling of embryo which increased in size and ruptured the seed coat. At this stage it was termed as spherule, which subsequently developed to form the protocorm and seedling. The percent germination varied in different media. In C. giganteum, it was 95% in M, 78.33% in MS and 75% in KC, whereas it was highest in MS (93.11%) followed by M (80.8%) and KC (72.62%) in R. retusa. The medium showing highest germination percentage was chosen to study the effect of varying concentrations of PGRs. Regeneration potential of different explants was also studied. These explants included protocorms, leaves, roots and seedlings in C. giganteum and protocorms, seedlings and young inflorescence in R. retusa. Protocorm like bodies (PLBs) regenerated in all the explants used except for seedlings in R. retusa, where these turned necrotic after 30 days of culture. Differentiation of PLBs at the base of seedlings in C. giganteum holds tremendous potential as the mother plant is not damaged. Moreover large number of PLBs can be produced by using optimized size of plant and PGRs concentration. In R. retusa, reversal of reproductive buds towards vegetative stage points towards the importance of developmental stage of reproductive buds when these could be diverted towards vegetative route under artificial conditions.