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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Biochemistry × Author: Reetu × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    ANTIOXIDANT PROPERTIES AND MOLECULAR DIVERSITY OF MEETHI NEEM (MURRAYA KOENIGII (L.) SPRENG)
    (CSKHPKV, Palampur, 2018-02-09) Reetu; Singh, Nageswer
    Fifteen wild Meethi Neem leaf samples collected at three stages viz. pre-flowering, flowering and fruiting stages from different locations of Kangra and Mandi district of Himachal Pradesh were analyzed to evaluate phytochemical constituents and to identify the genetic diversity. The range of variation for phytochemical constituents at three stages varied significantly viz. total chlorophyll (0.79 to 1.43, 1.31 to 1.94 and 0.88 to 1.10 mg/g), total carotenoids (30.55 to 49.25, 49.09 to 60.81 and 31.24 to 41.42 μg/g), ascorbic acid (7.01 to 9.82, 3.58 to 6.06 and 2.99 to 4.93 mg/100g), total phenol (170 to 304, 266 to 450 and 213 to 364 mg/g), simple phenol (93 to 163, 150 to 243 and 123 to 220 mg/g), tannins (76 to 143, 116 to 207 and 47 to 156 mg/g), flavonoids (15.17 to 33.40, 25.16 to 58.17 and 17.54 to 37.34 mg/g), polyphenol oxidase (0.030 to 0.058, 0.017 to 0.031 and 0.007 to 0.021 ΔOD/minute), antioxidant activity (4.01 to 7.42, 8.08 to 13.60 and 3.11 to 6.37 μg/ml), essential oil (0.64 to 0.89, 0.85 to 1.00 and 0.54 to 0.70 per cent) and quinones (2.05 to 2.97, 3.07 to 4.95 and 1.02 to 1.96 mM/min/g tissue). All parameters showed highest value at flowering stage except ascorbic acid and polyphenol oxidase activity. Clustering analysis with respect to biochemical constituents revealed that the geographically distinct collections of Meethi Neem were clustered together. For molecular characterization by ISSR, a total of 120 highly reproducible bands were obtained out of which 74 were polymorphic. The polymorphism ranged from 12.5 to 100 per cent. Dendrogram generated using UPGMA separated 10 collections into 2 major clusters with 46 to 100 per cent similarity. The overall analysis revealed that Meethi Neem collections from the same geographical location were genetically and biochemically diverse.