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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Animal Reproduction and Gynecology × Author: Rana, Pankaj × End date: 2019 × Start date: 2019 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    BACTERIOLOGICAL STUDIES ON PREPUTIAL WASHINGS AND AT VARIOUS SUBSEQUENT STAGES OF SEMEN PROCESSING IN COW BULLS
    (CSKHPKV, Palampur, 2019-05-17) Rana, Pankaj; Singh, Madhumeet
    The objective of the present study was to quantify the bacterial load at various stages of its collection and processing viz. preputial washings, neat semen after collection and further extended and frozen semen post-thaw. Samples were collected twice, each from 15 bulls, at weekly interval at all the stages of collection and processing. Four egg yolk and 4 extender samples were also collected. Samples were cultured over Plate Count Agar (PCA) for estimation of bacterial load. Bacterial isolation was done over blood agar, nutrient agar, EMB agar, MLA and BGA agar. Various gross, microbiological and biological tests were used to identify the isolates. Muller Hinton Agar (MHA) was used for antibiogram of isolates. Of all 124 samples processed, per cent of samples found positive for bacterial presence were 100, 100, 73.33, 43.33, 75 and 0 in preputial washings, neat semen, extended semen, frozen semen straws, egg yolk and extender, respectively. The mean bacterial load per ml of different ejaculates (n=30) consisting of preputial washings, neat semen, extended semen and frozen semen post-thaw were 166608.00±28351.80 (15000-868750), 4107.31±433.04 (750-10100), 116±33.39 (0-600) and 29.00±15.51 (0-400) CFU/ml, respectively. The predominant organism isolated were Staphylococcus spp. and Bacillus spp. in preputial washings and Staphylococcus spp. and Streptococcus spp. in neat, extended and frozen semen. Bacterial isolates were found to be sensitive to streptomycin, ciprofloxacin and marbofloxacin (100% each), gentamicin (93.33%), enrofloxacin (93.33%), ofloxacin (80.0%), ampicillin (40.0%) and lincomycin (39.99).